產品名稱:人源cDNA克隆
產品規格(ge):500μL甘油菌液
產品(pin)運輸條件:常溫(wen)
產品儲存條(tiao)件和期限:-80℃長期保存
產品特點:
? 人源(yuan)基因C-端融合(he) Flag & 6×His 標(biao)簽;
? 含有puromycin真核(he)抗性基因,可篩選穩定轉染細(xi)胞株;
? 含有(you)卡那霉素原(yuan)核抗性基因,可篩選重組克隆(long),推(tui)薦使用濃(nong)度(du)為30μg/ml。
產品使用:
將(jiang)甘油(you)菌在新鮮配制的固體(ti)培養基(ji)(相應抗性)中劃線,37℃培(pei)養(yang)箱(xiang)培(pei)養(yang)過(guo)(guo)夜,挑取3個(ge)單克隆(long),分別接種于相應(ying)抗性的液體培(pei)養(yang)基(ji)中,37℃搖床(chuang)培(pei)養(yang)過(guo)(guo)夜,然(ran)后進(jin)行(xing)質粒(li)的提取和驗證。
溫馨提示:考慮(lv)到實(shi)驗(yan)的嚴謹性,建議您拿到產(chan)品(pin)的第一(yi)時間進行測(ce)序確認。
ATGTTTAGAGCTGGGGAGGCCTCCAAACGCCCATTGCCTGGGCCGTCGCCCCCAAGGGTGCGGAGTGTGGAGGTTGCCCGGGGGAGGGCCGGCTACGGATTCACGCTTTCGGGACAGGCACCCTGTGTGCTCAGCTGCGTCATGAGAGGGAGCCCTGCGGATTTCGTGGGCCTCCGAGCTGGAGACCAGATACTTGCTGTCAATGAAATCAACGTGAAAAAAGCATCTCATGAAGATGTAGTGAAATTAATTGGGAAGTGCTCTGGTGTCCTTCACATGGTGATTGCTGAAGGCGTCGGCCGCTTCGAATCCTGTTCCAGTGATGAAGAAGGGGGACTCTATGAAGGAAAAGGCTGGCTGAAGCCCAAGCTGGATTCTAAAGCACTAGGTATAAACAGAGCAGAGCGAGTCGTGGAGGAAATGCAGTCTGGTGGAATTTTCAATATGATTTTTGAAAACCCGAGCCTTTGTGCGAGCAATTCAGAGCCCTTGAAATTGAAACAAAGATCCCTTTCAGAGTCGGCCGCAACTCGATTTGATGTTGGACATGAAAGTATAAATAATCCAAATCCCAACATGCTTTCTAAGGAGGAAATATCAAAAGTTATTCATGATGATTCGGTTTTCAGCATTGGACTAGAAAGTCATGACGATTTTGCATTGGATGCAAGTATTTTAAACGTGGCGATGATCGTGGGCTACTTAGGCTCCATTGAGCTTCCTTCCACGAGCTCCAACCTGGAGTCCGACAGCTTGCAAGCCATCCGCGGCTGCATGCGGCGCCTGCGGGCAGAGCAGAAAATCCACTCGCTGGTGACCATGAAGATCATGCACGACTGTGTGCAGCTGAGCACTGACAAGGCTGGAGTCGTGGCCGAGTACCCGGCCGAGAAGCTGGCCTTCAGCGCCGTGTGCCCGGACGACCGGCGATTTTTCGGGTTGGTTACCATGCAGACGAATGACGACGGGAGCCTGGCCCAGGAGGAGGAGGGCGCCCTGCGGACTTCCTGCCACGTGTTCATGGTGGACCCAGACTTGTTTAATCACAAGATCCACCAAGGCATTGCTCGGCGGTTTGGGTTTGAGTGCACGGCCGACCCAGACACCAATGGCTGTCTGGAATTCCCGGCGTCCTCCCTCCCCGTCCTGCAGTTCATCTCTGTCCTGTACCGAGACATGGGTGAGCTGATTGAGGGCATGCGGGCCCGCGCCTTTCTGGACGGGGACGCCGATGCCCACCAGAACAACAGCACCAGCAGCAACAGTGACAGCGGCATTGGGAACTTCCACCAGGAGGAGAAGAGCAACCGGGTCCTTGTGGTGGACCTGGGTGGGAGCTCGAGCAGACACGGCCCCGGAGGCAGCGCGTGGGACGGTGTGGGTGGGAGGGGTGCCCAGCCCTGGGGTGCTCCCTGGACTGGGCCCTTCTGTCCGGACCCCGAAGGGAGCCCCCCATTTGAGGCCGCTCATCAGACTGACAGGTTCTGGGACCTAAACAAGCACCTAGGGCCAGCCTCTCCTGTGGAGGTGCCCCCAGCTTCCTTGAGGAGCTCAGTCCCCCCTTCCAAGAGGGGCACCGTGGGTGCTGGCTGTGGTTTCAACCAGCGCTGGCTCCCGGTCCACGTGCTCCGGGAGTGGCAGTGCGGACACACCAGCGACCAGGACTCTTACACAGATTCCACCGATGGCTGGTCCAGCATCAACTGCGGCACACTGCCCCCTCCTATGAGCAAGATCCCCGCAGACCGCTACAGGGTGGAGGGCAGCTTCGCGCAGCCCCCGCTGAATGCCCCGAAGAGGGAGTGGTCCAGGAAGGCCTTTGGAATGCAAAGCATTTTTGGTCCCCATCGAAATGTTCGAAAGACTAAGGAAGATAAAAAGGGCTCAAAATTTGGGCGGGGAACTGGACTCACTCAGCCTTCTCAACGCACGTCTGCTCGGAGATCATTTGGGAGATCCAAGAGATTCAGTATCACTCGCTCCCTTGATGATCTTGAGTCTGCAACTGTGTCTGATGGCGAGTTGACGGGCGCCGACCTGAAGGACTGCGTCAGCAACAACAGCCTGAGCAGCAATGCCAGCCTCCCCAGCGTGCAGAGCTGCCGGCGCCTGCGTGAGAGGAGGGTCGCCAGCTGGGCCGTGTCCTTTGAGCGCCTGCTGCAGGACCCCGTCGGTGTCCGCTACTTCTCTGATTTTCTAAGGAAAGAATTCAGTGAAGAAAACATTTTATTCTGGCAGGCCTGTGAATATTTTAATCATGTTCCTGCACATGACAAAAAGGAGCTTTCCTACAGGGCCCGGGAGATTTTCAGTAAGTTTCTCTGCAGCAAAGCCACCACCCCGGTCAACATCGACAGCCAGGCCCAGCTAGCAGACGACGTCCTCCGCGCACCTCACCCAGACATGTTCAAGGAGCAGCAGCTGCAGATCTTCAATCTCATGAAGTTTGATAGCTACACTCGCTTTCTGAAGTCCCCGCTGTACCAGGAATGCATCCTGGCGGAAGTGGAGGGCCGTGCACTCCCGGACTCGCAGCAGGTCCCCAGCAGCCCGGCTTCCAAGCACAGCCTCGGTTCAGACCACTCCAGTGTGTCCACGCCAAAAAAGTTAAGTGGAAAATCAAAATCCGGCCGATCCCTGAATGAAGAGCTGGGGGATGAGGACAGCGAGAAGAAGCGGAAAGGCGCGTTTTTCTCGTGGTCGCGGACCAGGAGCACCGGGAGGTCCCAGAAAAAGAGGGAGCACGGGGACCACGCAGACGACGCCCTGCATGCCAATGGAGGCCTGTGTCGCCGAGAGTCGCAGGGCTCTGTGTCCTCTGCGGGGAGCCTGGACCTGTCGGAGGCCTGCAGGACTTTGGCACCCGAGAAGGACAAGGCCACCAAGCACTGCTGCATTCATCTCCCGGATGGGACATCCTGCGTGGTGGCTGTCAAGGCGGGCTTCTCCATCAAAGACATCCTGTCCGGACTCTGTGAGCGGCATGGCATCAACGGGGCGGCCGCGGACCTCTTCCTGGTGGGCGGGGACAAGCCTCTGGTGCTGCACCAAGACAGTAGCATCTTGGAGTCAAGGGACCTGCGCCTAGAAAAGCGCACCTTGTTTCGGCTGGATCTTGTTCCGATTAACCGGTCAGTGGGACTCAAGGCCAAGCCCACCAAGCCCGTCACGGAGGTGCTGCGGCCCGTGGTGGCCAGATACGGCCTGGACCTCAGTGGCCTGCTGGTGAGGCTGAGTGGAGAGAAGGAGCCCCTGGACCTTGGCGCCCCTATATCGAGTCTGGACGGACAGCGGGTTGTCTTGGAGGAGAAGGATCCTTCCAGAGGAAAGGCATCCGCAGATAAACAGAAAGGTGTGCCAGTGAAACAGAACACAGCTGTAAATTCCAGCTCCAGAAACCACTCGGCTACGGGAGAGGAAAGAACACTAGGCAAGTCTAATTCTATTAAAATAAAAGGAGAAAATGGAAAAAATGCTAGGGATCCCCGGCTTTCAAAGAGAGAAGAATCTATTGCAAAGATTGGGAAAAAAAAATATCAGAAAATTAATTTGGACGAAGCAGAGGAGTTTTTTGAGCTTATTTCCAAAGCTCAGAGCAACAGAGCAGATGACCAACGTGGGCTGCTAAGGAAGGAAGACCTGGTGTTGCCAGAGTTCCTCCGTTTACCTCCTGGTTCCACAGAACTCACCCTCCCCACTCCAGCTGCTGTGGCCAAGGGCTTTAGCAAGAGAAGCGCCACAGGCAACGGCCGGGAGAGCGCCTCCCAGCCTGGCGAGCAGTGGGAGCCAGTCCAGGAGAGCAGCGACAGCCCGTCCACCAGCCCGGGCTCAGCCTCCAGCCCCCCTGGACCTCCTGGGACGACCCCCCCCGGGCAGAAGTCTCCCAGCGGGCCCTTCTGCACTCCCCAGTCCCCCGTCTCCCTCGCGCAGGAGGGCACCGCCCAGATCTGGAAGAGGCAGTCTCAGGAAGTGGAGGCCGGGGGCATCCAGACGGTGGAGGATGAGCACGTGGCCGAGCTGACCCTGATGGGGGAGGGGGACATCAGCAGCCCCAACAGCACCTTGCTGCCGCCGCCCTCCACCCCCCAGGAAGTGCCAGGACCTTCCAGACCAGGTACCTCCAGGTTC
MFRAGEASKRPLPGPSPPRVRSVEVARGRAGYGFTLSGQAPCVLSCVMRGSPADFVGLRAGDQILAVNEINVKKASHEDVVKLIGKCSGVLHMVIAEGVGRFESCSSDEEGGLYEGKGWLKPKLDSKALGINRAERVVEEMQSGGIFNMIFENPSLCASNSEPLKLKQRSLSESAATRFDVGHESINNPNPNMLSKEEISKVIHDDSVFSIGLESHDDFALDASILNVAMIVGYLGSIELPSTSSNLESDSLQAIRGCMRRLRAEQKIHSLVTMKIMHDCVQLSTDKAGVVAEYPAEKLAFSAVCPDDRRFFGLVTMQTNDDGSLAQEEEGALRTSCHVFMVDPDLFNHKIHQGIARRFGFECTADPDTNGCLEFPASSLPVLQFISVLYRDMGELIEGMRARAFLDGDADAHQNNSTSSNSDSGIGNFHQEEKSNRVLVVDLGGSSSRHGPGGSAWDGVGGRGAQPWGAPWTGPFCPDPEGSPPFEAAHQTDRFWDLNKHLGPASPVEVPPASLRSSVPPSKRGTVGAGCGFNQRWLPVHVLREWQCGHTSDQDSYTDSTDGWSSINCGTLPPPMSKIPADRYRVEGSFAQPPLNAPKREWSRKAFGMQSIFGPHRNVRKTKEDKKGSKFGRGTGLTQPSQRTSARRSFGRSKRFSITRSLDDLESATVSDGELTGADLKDCVSNNSLSSNASLPSVQSCRRLRERRVASWAVSFERLLQDPVGVRYFSDFLRKEFSEENILFWQACEYFNHVPAHDKKELSYRAREIFSKFLCSKATTPVNIDSQAQLADDVLRAPHPDMFKEQQLQIFNLMKFDSYTRFLKSPLYQECILAEVEGRALPDSQQVPSSPASKHSLGSDHSSVSTPKKLSGKSKSGRSLNEELGDEDSEKKRKGAFFSWSRTRSTGRSQKKREHGDHADDALHANGGLCRRESQGSVSSAGSLDLSEACRTLAPEKDKATKHCCIHLPDGTSCVVAVKAGFSIKDILSGLCERHGINGAAADLFLVGGDKPLVLHQDSSILESRDLRLEKRTLFRLDLVPINRSVGLKAKPTKPVTEVLRPVVARYGLDLSGLLVRLSGEKEPLDLGAPISSLDGQRVVLEEKDPSRGKASADKQKGVPVKQNTAVNSSSRNHSATGEERTLGKSNSIKIKGENGKNARDPRLSKREESIAKIGKKKYQKINLDEAEEFFELISKAQSNRADDQRGLLRKEDLVLPEFLRLPPGSTELTLPTPAAVAKGFSKRSATGNGRESASQPGEQWEPVQESSDSPSTSPGSASSPPGPPGTTPPGQKSPSGPFCTPQSPVSLAQEGTAQIWKRQSQEVEAGGIQTVEDEHVAELTLMGEGDISSPNSTLLPPPSTPQEVPGPSRPGTSRF
維真生(sheng)物的人源cDNA克隆中,雖(sui)然我(wo)們(men)大(da)約70%的克隆與RefSeq或GeneBank所發(fa)表的基(ji)因(yin)列序(xu)完全一(yi)致,但(dan)也(ye)有(you)(you)些克隆含有(you)(you)天然的單核苷(gan)酸多態(tai)性而(er)包(bao)含一至多個(ge)SNPs, 因此與RefSeq或GeneBank所發表(biao)的列序會有些不同。我們(men)強(qiang)烈建議您在購買之前仔(zi)細將我們克隆的序列(lie)與您所(suo)要(yao)求的序列(lie)進行對比(bi),以決定(ding)所購買的克隆是否(fou)符(fu)合您的要求(qiu)(qiu)。如果(guo)我們預(yu)制的克隆不符(fu)合您的要求(qiu)(qiu),我們一般在1-2周內可以(yi)提供一(yi)個與(yu)您(nin)所要求的序列完全一(yi)致的克隆(long)。我們的每一(yi)個克隆(long)與(yu)RefSeq(2013)標準序列相比都含有一個(ge)完整的ORF(Opening Reading Frame)。因為RefSeq數據庫會不斷更(geng)新(xin)(xin),更(geng)新(xin)(xin)后(hou)的標準序列將來有(you)可能與我(wo)們(men)現有(you)的克隆序列產(chan)生差(cha)異。我(wo)們(men)所(suo)有(you)的克隆產(chan)品都經過二代(NextGen)測序驗證,并且保證網(wang)站上克(ke)隆(long)的(de)(de)序列與(yu)您收(shou)到的(de)(de)克(ke)隆(long)序列完全一(yi)致。在未經維真(zhen)生物(wu)書(shu)面(mian)給予授權前,人源ORF cDNA克(ke)隆不可轉售(shou)或贈予買方(fang)以外的(de)第三方(fang)。同時, 在使用任何維真產品(pin)之前,買方有責任(ren)確認該基因的(de)使用(yong)不會侵犯到第三方的(de)知識產(chan)權。此產(chan)品專門(men)為基礎研究所開(kai)發(fa),不適用(yong)于任(ren)何(he)治療或(huo)診(zhen)斷應用(yong)。