基因編號: NM_001199399
產品編號: CH804389
基因名稱: NEK1
基因大小: 3570 bp
分子量: 130.9 kDa
酶切位點: SgfI_MluI
價格: 詢價
供貨時間: 電詢 400-077-2566
產品名稱：人源cDNA克隆
產品規格：500μL甘油菌液
產品運輸條(tiao)件：常溫
產品(pin)儲存條件(jian)和期限(xian)：-80℃長期(qi)保存
產品特點(dian)：
? 人源基因C-端(duan)融合(he) Flag & 6×His 標簽；
? 含有(you)puromycin真核抗性(xing)基因，可(ke)篩(shai)選穩定轉染細胞株；
? 含有卡(ka)那霉素原核抗性基(ji)因，可篩(shai)選重組(zu)克(ke)隆(long)，推薦(jian)使用濃度(du)為30μg/ml。
產品使用：
將甘油菌在新鮮配制的(de)固(gu)體培養(yang)基（相應(ying)抗性）中(zhong)劃線，37℃培養(yang)箱培養(yang)過夜，挑取(qu)3個單(dan)克隆，分別接種于相(xiang)應(ying)抗性(xing)的液體培養(yang)基中，37℃搖床培養(yang)過夜，然后進行質粒(li)的提取(qu)和驗證。
溫馨(xin)提示：考慮(lv)到實(shi)驗的(de)嚴謹性，建議您(nin)拿到產品的第一(yi)時間(jian)進行測序確(que)認。
ATGGAGAAGTATGTTAGACTACAGAAGATTGGAGAAGGTTCATTTGGAAAAGCCATTCTTGTTAAATCTACAGAAGATGGCAGACAGTATGTTATCAAGGAAATTAACATCTCAAGAATGTCCAGTAAAGAAAGAGAAGAATCAAGGAGAGAAGTTGCAGTATTGGCAAACATGAAGCATCCAAATATTGTCCAGTATAGAGAATCATTTGAAGAAAATGGCTCTCTCTACATAGTAATGGATTACTGTGAGGGAGGGGATCTGTTTAAGCGAATAAATGCTCAGAAAGGCGTTTTGTTTCAAGAGGATCAGATTTTGGACTGGTTTGTACAGATATGTTTGGCCCTGAAACATGTACATGATAGAAAAATTCTTCATCGAGACATTAAATCTCAGAACATATTTTTAACTAAAGATGGAACAGTACAACTTGGAGATTTTGGAATTGCTAGAGTTCTTAATAGTACTGTAGAGCTGGCTCGAACTTGCATAGGGACCCCATACTACTTGTCACCTGAAATCTGTGAAAACAAACCTTACAATAATAAAAGTGACATTTGGGCTCTGGGGTGTGTCCTTTATGAGCTGTGTACACTTAAACATGCTTTTGAAGCTGGCAGTATGAAAAACCTGGTACTGAAGATAATATCTGGATCTTTTCCACCTGTGTCTTTGCATTATTCCTATGATCTCCGCAGTTTGGTGTCTCAGTTATTTAAAAGAAATCCTAGGGATAGACCATCAGTCAACTCCATATTGGAGAAAGGTTTTATAGCCAAACGCATTGAAAAGTTTCTCTCTCCTCAGCTTATTGCAGAAGAATTTTGTCTAAAAACATTTTCGAAGTTTGGATCACAGCCTATACCAGCTAAAAGACCAGCTTCAGGACAAAACTCGATTTCTGTTATGCCTGCTCAGAAAATTACAAAGCCTGCCGCTAAATATGGAATACCTTTAGCATATAAGAAATATGGAGATAAAAAATTACACGAAAAGAAACCACTGCAAAAACATAAACAGGCCCATCAAACTCCAGAGAAGAGAGTGAATACTGGAGAAGAAAGGAGGAAAATATCTGAGGAAGCAGCAAGAAAGAGAAGGCTGGAATTTATTGAAAAAGAAAAGAAACAAAAGGATCAGATTATTAGTTTAATGAAGGCTGAACAAATGAAAAGGCAAGAAAAGGAAAGGGCTCCTTTTCTGGGCAGTGGAGGGACTATAGCTCCATCATCTTTTTCTTCTCGAGGACAGTATGAACATTACCATGCCATTTTTGACCAAATGCAGCAACAAAGAGCAGAAGATAATGAAGCTAAATGGAAAAGAGAAATATATGGTCGAGGTCTTCCAGAAAGGCAAAAAGGGCAGCTAGCTGTAGAAAGAGCTAAACAAGTAGAAGAGTTCCTGCAGCGAAAACGGGAAGCTATGCAGAATAAAGCTCGAGCCGAAGGACATATGGTTTATCTGGCAAGACTGAGGCAAATAAGACTACAGAATTTCAATGAGCGCCAACAGATTAAAGCCAAACTTCGTGGTGAAAAGAAAGAAGCTAATCATTCTGAAGGACAAGAAGGAAGTGAAGAGGCTGACATGAGGCGCAAAAAAATCGAATCACTGAAGGCCCATGCAAATGCACGTGCTGCTGTACTAAAAGAACAACTAGAACGAAAGAGAAAGGAGGCTTATGAGAGAGAAAAAAAAGTGTGGGAAGAGCATTTGGTGGCTAAAGGAGTTAAGAGTTCTGATGTTTCTCCACCTTTGGGACAGCATGAAACAGGTGGCTCTCCATCAAAGCAACAGATGAGATCTGTTATTTCTGTAACTTCAGCTTTGAAAGAAGTTGGCGTGGACAGTAGTTTAACTGATACCCGGGAAACTTCAGAAGAGATGCAAAAGACCAACAATGCTATTTCAAGTAAGCGAGAAATACTTCGTAGATTAAATGAAAATCTTAAAGCTCAAGAAGATGAAAAAGGAAAGCAGAATCTCTCTGATACTTTTGAGATAAATGTTCATGAAGATGCCAAAGAGCATGAAAAAGAAAAATCAGTTTCATCTGATCGCAAGAAGTGGGAGGCAGGAGGTCAACTTGTGATTCCTCTGGATGAGTTAACACTAGATACATCCTTCTCTACAACTGAAAGACATACAGTGGGAGAAGTTATTAAATTAGGTCCTAATGGATCTCCAAGAAGAGCCTGGGGGAAAAGTCCGACAGATTCTGTTCTAAAGATACTTGGAGAAGCTGAACTACAACTTCAGACAGAACTATTAGAAAATACAACTATTAGAAGTGAGATTTCTCCCGAAGGGGAAAAGTACAAACCCTTAATTACTGGAGAAAAAAAAGTACAATGTATTTCACATGAAATAAACCCATCAGCTATTGTTGATTCTCCTGTTGAGACAAAAAGTCCCGAGTTCAGTGAGGCATCTCCACAGATGTCATTGAAACTGGAAGGAAATTTAGAAGAACCTGATGATTTGGAAACAGAAATTCTACAAGAGCCAAGTGGAACAAACAAAGATGAGAGCTTGCCATGCACTATTACTGATGTGTGGATTAGTGAGGAAAAAGAAACAAAGGAAACTCAGTCGGCAGATAGGATCACCATTCAGGAAAATGAAGTTTCTGAAGATGGAGTCTCGAGTACTGTGGACCAACTTAGTGACATTCATATAGAGCCTGGAACCAATGATTCTCAGCACTCTAAATGTGATGTAGATAAGTCTGTGCAACCGGAACCATTTTTCCATAAGGTGGTTCATTCTGAACACTTGAACTTAGTCCCTCAAGTTCAATCAGTTCAGTGTTCACCAGAAGAATCCTTTGCATTTCGATCTCACTCGCATTTACCACCAAAAAATAAAAACAAGAATTCCTTGCTGATTGGACTTTCAACTGGTCTGTTTGATGCAAACAACCCAAAGATGTTAAGGACATGTTCACTTCCAGATCTCTCAAAGCTGTTCAGAACCCTTATGGATGTTCCCACCGTAGGAGATGTTCGTCAAGACAATCTTGAAATAGATGAAATTGAAGATGAAAACATTAAAGAAGGACCTTCTGATTCTGAAGACATTGTGTTTGAAGAAACTGACACAGATTTACAAGAGCTGCAGGCCTCGATGGAACAGTTACTTAGGGAACAACCTGGTGAAGAATACAGTGAAGAAGAAGAGTCAGTCTTGAAGAACAGTGATGTGGAGCCAACTGCAAATGGGACAGATGTGGCAGATGAAGATGACAATCCCAGCAGTGAAAGTGCCCTGAACGAAGAATGGCACTCAGATAACAGTGATGGTGAAATTGCTAGTGAATGTGAATGCGATAGTGTCTTTAACCATTTAGAGGAACTGAGACTTCATCTGGAGCAGGAAATGGGCTTTGAAAAATTCTTTGAGGTTTATGAGAAAATAAAGGCTATTCATGAAGATGAAGATGAAAATATTGAAATTTGTTCAAAAATAGTTCAAAATATTTTGGGAAATGAACATCAGCATCTTTATGCCAAGATTCTTCATTTAGTCATGGCAGATGGAGCCTACCAAGAAGATAATGATGAA
MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMVYLARLRQIRLQNFNERQQIKAKLRGEKKEANHSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTFEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSEISPEGEKYKPLITGEKKVQCISHEINPSAIVDSPVETKSPEFSEASPQMSLKLEGNLEEPDDLETEILQEPSGTNKDESLPCTITDVWISEEKETKETQSADRITIQENEVSEDGVSSTVDQLSDIHIEPGTNDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGEIASECECDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE
維真生(sheng)物的人(ren)源cDNA克隆中，雖然我們大約70%的克(ke)隆(long)與RefSeq或(huo)GeneBank所發表(biao)的(de)基因列(lie)序完全一致,但(dan)也有些克隆含有天(tian)然的單核苷(gan)酸多(duo)態性而(er)包含一(yi)至多(duo)個SNPs, 因(yin)此與(yu)RefSeq或GeneBank所發表(biao)的列序會有些不同。我們強烈建議您在購買之前仔細將我們克(ke)隆的序列(lie)與您所要求(qiu)的序列(lie)進(jin)行對比，以決定所購買的(de)克隆是否(fou)符(fu)(fu)合(he)您的(de)要(yao)(yao)求。如果(guo)我們預制(zhi)的(de)克隆不符(fu)(fu)合(he)您的(de)要(yao)(yao)求，我們一般在1-2周(zhou)內可(ke)以提供一(yi)個與您所要求的序列完全一(yi)致(zhi)的克(ke)隆(long)。我們(men)的每(mei)一(yi)個克(ke)隆(long)與RefSeq（2013）標準序列相比都含有一(yi)個完整的ORF(Opening Reading Frame)。因為(wei)RefSeq數據(ju)庫會不斷(duan)更新，更新后(hou)的(de)標(biao)準(zhun)序列將來有可能與我(wo)們現(xian)有的(de)克隆序列產(chan)(chan)生差(cha)異。我(wo)們所(suo)有的(de)克隆產(chan)(chan)品都經過二代（NextGen）測序驗(yan)證，并且保證網站上克(ke)隆的(de)序列(lie)與(yu)您收到(dao)的(de)克(ke)隆序列(lie)完(wan)全一致(zhi)。在未(wei)經維真(zhen)生物(wu)書面給予(yu)授權前，人源ORF cDNA克隆(long)不可轉售或贈予買方以外的第三方。同時, 在使(shi)用任何維真產(chan)品之前(qian)，買(mai)方(fang)有責(ze)任確認該基因的(de)使用(yong)不(bu)會侵犯到第(di)三方(fang)的(de)知識產(chan)權。此產(chan)品專門為基礎研(yan)究所開(kai)發，不(bu)適用(yong)于任何治療或(huo)診斷應(ying)用(yong)。
400-077-2566
service@wzbio.cn
