產品名稱:人源cDNA克隆
產品(pin)規格:500μL甘油菌液
產品運輸條件:常溫(wen)
產品儲存條件和期限:-80℃長期保存(cun)
產(chan)品特點:
? 人源基因(yin)C-端融(rong)合 Flag & 6×His 標簽;
? 含(han)有(you)puromycin真核抗性(xing)基因,可篩選穩(wen)定轉染細胞株;
? 含(han)有卡(ka)那(nei)霉素原核抗性基因,可篩(shai)選重組克(ke)隆,推薦使(shi)用濃(nong)度為(wei)30μg/ml。
產品使(shi)用(yong):
將甘(gan)油菌在新鮮配制的固(gu)體(ti)培養基(相(xiang)應抗(kang)性)中劃線,37℃培養箱培養過(guo)夜(ye)(ye),挑取3個(ge)單克隆,分(fen)別接種于相應抗性的液體培養基中(zhong),37℃搖(yao)床(chuang)培養過(guo)夜(ye)(ye),然后進行(xing)質粒的提取和驗證(zheng)。
溫(wen)馨提示:考慮到實驗的嚴謹性,建議您拿到產品的第(di)一時間(jian)進(jin)行測序確認(ren)。
CGCCATGGCATCCTGGTTATATGAATGTCTTTGTGAAGCTGAACTTGCACAGTATTATTCTCATTTCACTGCCCTTGGCCTTCAGAAAATAGATGAATTAGCCAAGATTACAATGAAGGACTACTCCAAATTAGGAGTCCATGACATGAACGACCGCAAACGTCTCTTCCAACTTATCAAAATTATTAAGATTATGCAAGAAGAAGATAAAGCAGTCAGTATCCCAGAGCGTCATCTTCAGACAAGCAGCCTGCGCATCAAATCTCAGGAATTAAGATCTGGCCCTCGCAGACAGCTGAATTTTGATTCTCCTGCTGACAATAAAGACAGAAATGCCAGCAATGATGGGTTTGAAATGTGCAGTTTATCAGATTTCTCTGCAAATGAACAGAAGTCCACTTACCTAAAAGTGCTAGAACACGTGCTACCAGATGATTCCCAGTACCATACAAAAACAGGAATTCTGAATGCCACAGCTGGTGATTCCTATGTGCAAACAGAAATCAGCACTTCACTCTTTTCACCAAATTACCTTTCTGCAATACTGGGGGATTGTGATATTCCCATTATTCAAAGAATCTCTCATGTTTCAGGGTATAACTATGGAATCCCTCATTCTTGTATCAGACAGAACACTTCAGAGAAACAGAATCCTTGGACTGAGATGGAGAAAATCAGAGTTTGTGTTCGAAAACGCCCCCTGGGCATGAGGGAGGTACGTCGTGGAGAAATTAATATTATTACTGTAGAAGACAAAGAAACTCTACTTGTGCATGAGAAGAAAGAAGCAGTTGACCTCACTCAATATATTCTGCAGCATGTTTTTTATTTTGATGAAGTCTTTGGTGAGGCGTGCACCAATCAGGATGTATACATGAAGACTACTCACCCACTTATTCAGCATATTTTCAATGGAGGCAATGCCACTTGCTTTGCTTATGGACAGACAGGTGCTGGAAAGACCTACACCATGATAGGAACTCATGAGAACCCAGGATTGTATGCTCTAGCTGCCAAAGATATCTTCAGGCAACTAGAAGTGTCCCAGCCAAGAAAGCACCTCTTTGTGTGGATCAGCTTCTATGAAATTTACTGTGGACAGCTTTATGACCTCCTAAATAGAAGAAAAAGGCTCTTTGCAAGAGAAGATAGCAAGCACATGGTGCAGATAGTGGGACTGCAAGAGCTTCAGGTGGACAGTGTGGAGCTCCTCTTAGAGGTGATCTTAAAGGGCAGCAAGGAGCGCAGCACTGGGGCCACTGGAGTTAATGCAGACTCCTCCCGCTCCCATGCCGTCATCCAAATTCAGATCAAAGATTCAGCCAAGAGGACATTTGGCAGGATCTCTTTTATTGACTTGGCTGGCAGTGAAAGAGCAGCAGATGCAAGGGACTCAGATAGACAGACAAAGATGGAAGGTGCAGAAATAAATCAGAGTCTACTGGCTCTGAAGGAATGTATCCGAGCACTGGATCAGGAACACACCCATACTCCCTTCAGGCAAAGCAAACTAACTCAGGTCCTGAAGGACTCTTTCATCGGCAATGCCAAAACCTGCATGATCGCCAACATCTCACCAAGCCACGTGGCCACTGAACACACTCTCAACACCTTGCGCTATGCTGACCGGGTCAAAGAACTAAAGAAAGGCATTAAGTGTTGCACTTCAGTTACCAGTCGAAATCGGACATCTGGAAACTCCTCTCCAAAACGAATTCAGAGCTCCCCTGGGGCTTTGTCAGAGGACAAATGTTCTCCCAAAAAAGTCAAGCTGGGATTTCAGCAGTCACTCACAGTGGCAGCCCCTGGTTCCACGAGAGGGAAGGTCCATCCTCTGACCAGCCACCCACCCAACATTCCTTTTACTTCTGCACCTAAGGTCTCTGGTAAAAGGGGTGGCTCCAGAGGGAGTCCTTCACAAGAGTGGGTCATTCATGCTAGCCCTGTGAAAGGAACTGTGCGCTCTGGACATGTGGCCAAAAAAAAGCCAGAAGAGTCAGCACCATTGTGCTCTGAGAAAAATCGAATGGGCAACAAAACTGTCCTTGGGTGGGAAAGCAGGGCCTCAGGCCCAGGAGAAGGCCTAGTGCGTGGTAAGCTGTCCACCAAGTGCAAGAAAGTGCAGACAGTGCAGCCAGTACAGAAGCAGCTTGTGTCTCGAGTTGAGCTCTCCTTTGGCAACGCCCACCACAGGGCTGAGTACAGTCAAGACAGCCAGAGGGGCACGCCTGCTAGGCCTGCCTCTGAAGCTTGGACAAACATCCCGCCACATCAGAAGGAGAGGGAGGAACATCTGCGTTTCTATCACCAGCAGTTCCAACAGCCACCTCTCCTCCAACAGAAGTTAAAATACCAACCACTGAAAAGGTCTTTACGCCAGTACAGGCCCCCAGAGGGTCAGCTCACGAATGAGACTCCGCCTCTGTTCCACTCTTACTCTGAAAACCATGATGGAGCCCAAGTAGAGGAACTTGATGACAGTGATTTCAGTGAAGATTCTTTTTCACACATCTTTAGTCAGAGGGCCACAAAGCAAAGGAACACCCTGGAGAATAGCGAAGACTCATTCTTCCTGCACCAGACGTGGGGACAGGGTCCTGAGAAGCAGGTGGCAGAAAGACAGCAGAGTCTGTTTTCTAGCCCCAGGACAGGTGACAAGAAAGATCTAACTAAAAGCTGGGTGGACTCCAGGGACCCCATAAACCACAGAAGAGCAGCACTCGATCACAGCTGCAGCCCAAGTAAGGGGCCCGTGGACTGGAGCAGAGAGAACTCTACTTCCTCAGGGCCTTCTCCCAGAGACAGCCTGGCAGAGAAGCCATACTGTTCACAGGTAGATTTCATATATAGACAGGAAAGAGGTGGAGGCTCTTCCTTTGATCTCAGAAAGGATGCCTCCCAAAGTGAGGTTTCTGGGGAGAATGAGGGCAACTTGCCATCCCCAGAGGAAGATGGTTTCACTATCTCATTGTCCCACGTTGCAGTTCCTGGATCCCCAGACCAAAGAGACACAGTCACCACACCTCTGAGAGAAGTCAGTGCAGACGGCCCAATCCAGGTGACCAGCACTGTGAAAAACGGTCATGCTGTCCCAGGAGAGGATCCTAGGGGGCAGTTAGGCACGCATGCTGAATATGCTTCTGGACTCATGTCTCCCCTCACCATGTCCCTCCTGGAGAACCCAGACAACGAAGGGTCTCCTCCCTCGGAGCAGCTGGTCCAGGATGGGGCTACGCACAGTCTAGTGGCAGAGAGCACAGGAGGCCCAGTTGTGAGCCACACAGTGCCATCTGGTGATCAAGAGGCAGCCTTGCCAGTGTCTTCAGCAACTAGGCACCTGTGGCTGTCCTCATCTCCCCCTGATAATAAGCCTGGTGGTGATCTTCCAGCTCTGTCCCCATCACCCATCCGTCAGCACCCAGCTGACAAGCTGCCCAGCAGGGAGGCAGACCTAGGAGAGGCCTGCCAGAGCAGAGAGACTGTACTTTTCTCCCACGAACACATGGGTAGTGAGCAGTATGATGCTGATGCAGAGGAGACGGGGCTGGATGGCTCCTGGGGTTTCCCAGGAAAGCCCTTCACCACCATACATATGGGGGTACCCCATTCTGGACCTACACTCACCCCACGAACAGGAAGTAGTGATGTGGCTGACCAGCTCTGGGCCCAGGAGAGAAAACATCCTACAAGGCTTGGTTGGCAGGAGTTTGGTTTGTCCACAGACCCCATCAAGTTGCCCTGCAACAGTGAAAATGTCACATGGCTCAAACCCAGGCCGATCTCAAGGTGCTTAGCAAGGCCAAGTTCTCCCTTGGTTCCCAGCTGCTCTCCCAAGACTGCAGGGACACTCCGTCAGCCCACCCTGGAGCAAGCGCAGCAGGTGGTCATCCGAGCACACCAGGAACAGCTGGATGAAATGGCTGAGCTCGGCTTCAAGGAGGAGACGCTGATGAGCCAGCTGGCTTCTAATGATTTTGAAGATTTTGTGACCCAGCTGGATGAAATCATGGTTCTGAAATCCAAGTGTATCCAGAGTCTGAGGAGCCAGCTGCAGCTCTATCTCACCTGCCACGGGCCCACCGCAGCCCCTGAGGGAACagtgccgtcta
MASWLYECLCEAELAQYYSHFTALGLQKIDELAKITMKDYSKLGVHDMNDRKRLFQLIKIIKIMQEEDKAVSIPERHLQTSSLRIKSQELRSGPRRQLNFDSPADNKDRNASNDGFEMCSLSDFSANEQKSTYLKVLEHVLPDDSQYHTKTGILNATAGDSYVQTEISTSLFSPNYLSAILGDCDIPIIQRISHVSGYNYGIPHSCIRQNTSEKQNPWTEMEKIRVCVRKRPLGMREVRRGEINIITVEDKETLLVHEKKEAVDLTQYILQHVFYFDEVFGEACTNQDVYMKTTHPLIQHIFNGGNATCFAYGQTGAGKTYTMIGTHENPGLYALAAKDIFRQLEVSQPRKHLFVWISFYEIYCGQLYDLLNRRKRLFAREDSKHMVQIVGLQELQVDSVELLLEVILKGSKERSTGATGVNADSSRSHAVIQIQIKDSAKRTFGRISFIDLAGSERAADARDSDRQTKMEGAEINQSLLALKECIRALDQEHTHTPFRQSKLTQVLKDSFIGNAKTCMIANISPSHVATEHTLNTLRYADRVKELKKGIKCCTSVTSRNRTSGNSSPKRIQSSPGALSEDKCSPKKVKLGFQQSLTVAAPGSTRGKVHPLTSHPPNIPFTSAPKVSGKRGGSRGSPSQEWVIHASPVKGTVRSGHVAKKKPEESAPLCSEKNRMGNKTVLGWESRASGPGEGLVRGKLSTKCKKVQTVQPVQKQLVSRVELSFGNAHHRAEYSQDSQRGTPARPASEAWTNIPPHQKEREEHLRFYHQQFQQPPLLQQKLKYQPLKRSLRQYRPPEGQLTNETPPLFHSYSENHDGAQVEELDDSDFSEDSFSHIFSQRATKQRNTLENSEDSFFLHQTWGQGPEKQVAERQQSLFSSPRTGDKKDLTKSWVDSRDPINHRRAALDHSCSPSKGPVDWSRENSTSSGPSPRDSLAEKPYCSQVDFIYRQERGGGSSFDLRKDASQSEVSGENEGNLPSPEEDGFTISLSHVAVPGSPDQRDTVTTPLREVSADGPIQVTSTVKNGHAVPGEDPRGQLGTHAEYASGLMSPLTMSLLENPDNEGSPPSEQLVQDGATHSLVAESTGGPVVSHTVPSGDQEAALPVSSATRHLWLSSSPPDNKPGGDLPALSPSPIRQHPADKLPSREADLGEACQSRETVLFSHEHMGSEQYDADAEETGLDGSWGFPGKPFTTIHMGVPHSGPTLTPRTGSSDVADQLWAQERKHPTRLGWQEFGLSTDPIKLPCNSENVTWLKPRPISRCLARPSSPLVPSCSPKTAGTLRQPTLEQAQQVVIRAHQEQLDEMAELGFKEETLMSQLASNDFEDFVTQLDEIMVLKSKCIQSLRSQLQLYLTCHGPTAAPEGTVPS
維真生物的人源cDNA克隆中,雖(sui)然(ran)我們大約70%的克隆(long)與RefSeq或GeneBank所發(fa)表(biao)的基(ji)因列序完全一致,但也(ye)有些克(ke)隆(long)含有天然的單核苷酸多態性而包含一(yi)至多個SNPs, 因此與(yu)RefSeq或GeneBank所發表的(de)列(lie)序會有些不同。我們強(qiang)烈建議您在購買(mai)之前(qian)仔細將我們(men)克(ke)隆(long)的序列與您所要(yao)求的序列進行對比,以決定所(suo)購(gou)買(mai)的克(ke)隆(long)是否符合您的要(yao)求。如(ru)果(guo)我(wo)們預制的克(ke)隆(long)不符合您的要(yao)求,我(wo)們一般在1-2周內可以提供(gong)一(yi)個(ge)與您所要求(qiu)的序(xu)列完全一(yi)致的克(ke)隆(long)。我們的每一(yi)個(ge)克(ke)隆(long)與RefSeq(2013)標準序(xu)列相(xiang)比都(dou)含有一(yi)個完整(zheng)的ORF(Opening Reading Frame)。因為(wei)RefSeq數據庫會不斷(duan)更(geng)(geng)新,更(geng)(geng)新后(hou)的標(biao)準序列將(jiang)來(lai)有(you)可(ke)能與我們現有(you)的克(ke)隆(long)序列產生差(cha)異。我們所(suo)有(you)的克(ke)隆(long)產品都經過二代(NextGen)測序驗證,并且(qie)保證網站上克隆的序列(lie)與您收到(dao)的克隆序列(lie)完全一致。在未經維真生物書面給予(yu)授權前,人源(yuan)ORF cDNA克(ke)隆不可轉售(shou)或(huo)贈予買方以外的(de)第三方。同時, 在使(shi)用任何維真產品之前(qian),買方(fang)(fang)有責任(ren)確(que)認(ren)該基因(yin)的使用不會侵犯到第(di)三(san)方(fang)(fang)的知識產(chan)權(quan)。此產(chan)品(pin)專門為基礎研究所開發(fa),不適用于任(ren)何治療或診斷應用。