產品名稱:人源cDNA克隆
產品(pin)規格:500μL甘油菌液
產品運輸條件(jian):常溫
產品儲(chu)存(cun)條(tiao)件和期限:-80℃長期保存
產品特點:
? 人源(yuan)基因C-端融合 Flag & 6×His 標簽;
? 含有puromycin真核抗(kang)性基因(yin),可(ke)篩(shai)選穩定轉染(ran)細(xi)胞株(zhu);
? 含有卡那霉(mei)素原(yuan)核抗性基因,可篩選(xuan)重組克隆,推薦使(shi)用濃度為30μg/ml。
產品使用:
將(jiang)甘油菌在新鮮配(pei)制的固體培養基(ji)(相應抗(kang)性)中劃線(xian),37℃培(pei)養(yang)箱(xiang)培(pei)養(yang)過夜,挑取3個單(dan)克隆,分別(bie)接種于相應抗性的液體培(pei)養(yang)基(ji)中,37℃搖床培(pei)養(yang)過夜,然后(hou)進(jin)行質粒的提取和(he)驗證。
溫馨提示:考慮(lv)到實驗的嚴謹性,建議您拿(na)到產品的第一時間進行測序確認。
ATGTGGATACTGGCTCTCTCCTTGTTCCAGAGCTTCGCGAATGTTTTCAGTGAAGACCTACACTCCAGCCTCTACTTTGTCAATGCATCTCTGCAAGAGGTAGTGTTTGCCAGCACCACGGGGACTCTGGTGCCCTGCCCCGCAGCAGGCATCCCTCCTGTGACTCTCAGATGGTACCTAGCCACGGGCGAGGAGATCTACGATGTCCCCGGGATCCGCCACGTCCACCCCAACGGCACTCTCCAAATTTTCCCCTTCCCTCCTTCAAGCTTCAGTACCTTAATCCATGATAATACTTATTATTGCACAGCTGAAAATCCTTCAGGGAAAATTAGAAGTCAGGATGTCCACATCAAGGCTGTTTTACGGGAGCCCTATACAGTCCGTGTGGAGGACCAGAAAACCATGAGAGGCAATGTTGCGGTCTTCAAGTGCATTATCCCCTCCTCGGTGGAGGCGTACATCACTGTCGTCTCATGGGAGAAAGACACTGTTTCACTTGTCTCAGGATCTAGATTTCTCATCACATCCACGGGAGCCTTGTATATTAAAGATGTACAGAATGAAGATGGATTGTATAACTACCGCTGCATCACGCGGCATCGATACACCGGAGAGACGAGGCAGAGCAACAGCGCCAGACTTTTTGTATCAGACCCAGCGAACTCAGCCCCATCCATACTGGATGGGTTTGACCATCGCAAAGCCATGGCTGGGCAGCGTGTGGAGCTGCCTTGCAAAGCGCTCGGGCACCCTGAGCCAGATTACCGCTGGCTGAAGGACAACATGCCCCTGGAACTTTCAGGGAGGTTCCAGAAGACCGTGACGGGGCTGCTCATTGAGAACATTCGCCCCTCGGACTCAGGCAGCTATGTTTGTGAAGTGTCCAACAGATACGGAACTGCTAAGGTGATAGGCCGCCTGTACGTGAAACAGCCACTGAAAGCCACCATCAGTCCCAGGAAGGTTAAAAGCAGCGTGGGTAGCCAAGTTTCCTTGTCCTGCAGCGTGACAGGAACTGAGGACCAGGAACTCTCCTGGTACCGCAATGGTGAAATCCTCAACCCTGGAAAAAATGTGAGGATCACAGGGATCAACCACGAAAACCTTATAATGGATCACATGGTCAAAAGTGACGGGGGCGCATACCAGTGCTTTGTGCGCAAGGACAAGCTGTCCGCTCAAGACTATGTGCAGGTGGTCCTTGAAGATGGAACTCCCAAAATTATTTCTGCCTTTAGTGAAAAGGTGGTGAGTCCAGCAGAGCCGGTTTCCCTTATGTGCAACGTGAAGGGAACACCTTTGCCCACGATCACGTGGACCCTGGACGATGACCCGATTCTCAAGGGTGGCAGTCACCGCATCAGCCAGATGATCACGTCGGAGGGGAACGTGGTCAGCTACCTGAACATCTCCAGCTCCCAGGTCCGGGACGGGGGAGTCTACCGCTGCACTGCCAACAACTCGGCGGGAGTCGTCCTGTACCAGGCTCGAATAAACGTAAGAGGGCCTGCAAGCATTCGACCAATGAAAAACATCACAGCAATAGCAGGACGGGACACATACATTCACTGTCGTGTGATTGGCTATCCGTATTACTCCATTAAATGGTACAAGAACTCTAACCTGCTTCCTTTCAACCACCGCCAAGTGGCATTTGAGAACAATGGAACTCTTAAACTTTCAGATGTGCAAAAGGAAGTGGACGAGGGGGAGTACACGTGCAACGTGTTGGTTCAACCACAACTCTCCACCAGCCAGAGCGTCCACGTGACCGTGAAAGTTCCGCCTTTCATACAACCCTTTGAGTTTCCAAGATTCTCCATTGGGCAGCGGGTCTTCATCCCCTGTGTTGTGGTCTCAGGGGACTTACCCATCACGATCACCTGGCAGAAGGATGGCCGGCCAATCCCTGGGAGCCTTGGGGTGACCATTGACAATATTGACTTCACGAGCTCCTTGAGGATTTCCAATCTCTCGCTCATGCACAATGGGAATTACACCTGCATAGCCCGGAATGAGGCCGCCGCTGTGGAGCACCAAAGCCAGTTGATTGTCAGAGTTCCTCCCAAGTTTGTGGTTCAGCCACGGGACCAGGACGGGATTTATGGCAAAGCAGTCATCCTCAATTGTTCTGCTGAGGGTTACCCTGTACCTACCATCGTGTGGAAATTCTCTAAAGGTGCTGGGGTTCCCCAGTTCCAGCCAATTGCCCTAAATGGCCGAATCCAAGTTCTCAGCAATGGGTCGTTGCTGATCAAGCATGTCGTGGAGGAAGACAGTGGCTACTACCTCTGCAAGGTCAGCAACGATGTGGGCGCAGACGTCAGCAAGTCCATGTACCTCACGGTTAAAATTCCTGCGATGATAACATCCTATCCAAATACTACCCTGGCCACGCAGGGGCAGAAAAAGGAGATGAGCTGCACGGCGCATGGTGAGAAGCCCATTATAGTCCGCTGGGAGAAGGAGGACCGAATCATTAACCCTGAGATGGCCCGTTATCTTGTGTCCACCAAGGAGGTGGGAGAAGAGGTGATTTCTACTCTGCAGATTTTGCCAACTGTGAGAGAAGATTCTGGTTTCTTTTCCTGCCATGCTATTAATTCTTATGGGGAGGACCGTGGAATAATTCAGCTCACAGTGCAAGAGCCCCCAGACCCTCCCGAAATTGAGATCAAAGATGTCAAAGCACGCACAATTACGCTCAGGTGGACCATGGGGTTTGATGGAAACAGTCCCATCACAGGCTACGATATTGAATGCAAAAATAAATCAGACTCCTGGGATTCTGCTCAGAGAACCAAAGATGTTTCCCCTCAGCTGAACTCGGCCACCATCATTGATATCCACCCTTCCTCCACCTACAGCATCCGCATGTACGCCAAGAACCGGATTGGCAAGAGCGAGCCCAGCAACGAGCTCACCATCACGGCGGACGAGGCAGCTCCTGATGGTCCACCTCAGGAAGTTCACCTGGAGCCTATATCATCTCAGAGCATCAGGGTCACATGGAAGGCTCCCAAGAAACATTTGCAAAATGGGATTATCCGTGGCTACCAAATAGGTTACCGAGAGTACAGCACTGGGGGTAACTTCCAATTCAACATTATCAGTGTCGACACCAGCGGGGACAGTGAGGTTTACACCCTGGACAACCTGAATAAGTTCACTCAGTACGGCCTGGTGGTGCAGGCCTGTAACCGGGCCGGCACGGGGCCTTCTTCTCAGGAAATCATCACCACCACTCTCGAGGATGTGCCCAGTTACCCCCCCGAAAATGTCCAAGCCATAGCAACATCACCAGAAAGCATATCAATATCCTGGTCCACACTTTCCAAGGAAGCCTTGAATGGAATTCTCCAGGGGTTCAGAGTCATTTACTGGGCCAACCTCATGGACGGAGAGCTGGGTGAGATTAAAAACATCACCACCACACAGCCTTCACTGGAGCTGGACGGGCTGGAAAAGTACACCAACTACAGCATCCAGGTGCTGGCCTTCACCCGCGCAGGAGACGGGGTCAGGAGTGAGCAGATCTTCACCCGGACCAAAGAGGATGTTCCAGGTCCTCCCGCGGGTGTGAAGGCAGCGGCGGCCTCAGCCTCCATGGTCTTTGTGTCCTGGCTTCCCCCTCTCAAGCTGAACGGCATCATCCGAAAGTACACTGTATTCTGCTCCCACCCCTATCCCACAGTGATCAGCGAGTTTGAGGCCTCTCCCGACTCGTTTTCCTACAGAATTCCCAACCTGAGTAGGAATCGTCAGTACAGCGTCTGGGTGGTGGCTGTTACTTCAGCCGGAAGAGGCAACAGCAGTGAAATCATCACAGTCGAGCCACTAGCAAAAGCTCCTGCACGAATCCTGACCTTCAGTGGGACAGTGACTACTCCATGGATGAAAGACATTGTCTTGCCTTGTAAGGCTGTTGGGGACCCTTCTCCTGCAGTCAAATGGATGAAAGACAGTAACGGGACACCCAGTCTAGTAACGATTGATGGGCGGAGGAGCATCTTTAGCAACGGAAGCTTCATTATTCGCACGGTGAAAGCAGAAGACTCCGGCTATTACAGCTGCATTGCCAATAACAACTGGGGATCTGATGAAATTATTTTAAACTTACAAGTACAAGTTCCACCAGATCAGCCTCGGCTTACAGTCTCCAAGACCACGTCTTCCTCCATCACCCTTTCTTGGCTCCCTGGAGACAACGGGGGCAGCTCTATCAGAGGATACATACTGCAGTACTCCGAGGACAATAGTGAGCAGTGGGGGAGTTTTCCAATCAGCCCCAGCGAACGTTCCTATCGCTTGGAAAATCTCAAATGTGGGACTTGGTATAAGTTCACACTGACAGCCCAAAATGGAGTGGGCCCAGGGCGCATAAGTGAAATCATAGAAGCAAAGACCTTAGGAAAAGAGCCCCAGTTCTCAAAGGAGCAGGAGCTGTTTGCCAGCATCAACACCACACGCGTGAGGCTGAACCTCATTGGCTGGAATGATGGCGGCTGCCCCATCACCTCCTTCACACTAGAGTACAGGCCCTTTGGGACCACAGTTTGGACCACAGCTCAGAGGACCTCTCTCTCCAAGTCCTACATCCTGTATGACCTGCAGGAAGCCACCTGGTATGAGCTGCAGATGCGGGTGTGCAACAGTGCGGGCTGCGCGGAGAAGCAGGCCAACTTCGCTACGCTGAACTACGATGGCAGTACAATTCCTCCACTCATTAAGTCAGTTGTCCAAAACGAAGAAGGGCTGACGACCAACGAGGGGCTCAAGATGCTGGTGACCATCTCCTGTATCCTGGTGGGGGTCTTGCTGCTGTTTGTGCTCCTGCTGGTTGTGCGGAGGAGGCGGCGGGAGCAGAGGCTAAAGAGGCTGCGAGATGCAAAGAGTTTAGCTGAAATGCTCATGAGTAAGAATACCCGGACTTCAGATACGTTAAGCAAGCAACAGCAGACCCTGCGAATGCACATCGACATACCCAGGGCTCAGCTTTTGATTGAAGAGAGAGACACGATGGAGACCATTGATGATCGCTCCACGGTTCTGTTGACGGATGCTGACTTTGGAGAGGCAGCTAAGCAGAAGTCCCTGACGGTCACTCACACGGTCCATTACCAATCGGTGTCTCAGGCCACTGGGCCCTTAGTGGATGTTTCAGACGCTCGGCCGGGAACGAATCCCACCACCAGGAGGAATGCCAAGGCTGGGCCCACAGCGAGAAACCGCTATGCCAGCCAGTGGACCCTCAACCGACCCCACCCCACCATCTCAGCACACACCCTCACCACAGACTGGAGGCTGCCAACACCCAGGGCTGCAGGATCAGTAGACAAAGAGAGCGACAGTTACAGCGTCAGCCCCTCGCAAGACACAGATCGAGCAAGAAGCAGCATGGTCTCCACAGAAAGTGCCTCCTCCACTTACGAAGAACTGGCCAGGGCCTACGAACACGCCAAGATGGAAGAGCAACTGAGGCACGCCAAGTTCACCATCACGGAGTGCTTCATATCAGACACGTCATCGGAGCAGTTGACGGCAGGGACAAATGAGTACACGGACAGTCTGACCTCCAGCACCCCTTCCGAATCGGGAATCTGCAGGTTCACTGCATCTCCCCCCAAACCTCAGGATGGAGGAAGAGTAATGAATATGGCAGTTCCAAAGGCACATCGGCCAGGTGACCTCATACATTTGCCTCCATACCTTAGAATGGACTTTTTGTTAAACCGAGGTGGTCCAGGCACCAGCAGGGACCTGAGCTTAGGACAAGCATGCTTGGAACCTCAGAAAAGCCGGACCCTGAAGCGCCCCACGGTCCTGGAGCCCATCCCGATGGAAGCCGCCTCCTCCGCCTCCTCCACGAGAGAAGGACAGTCGTGGCAGCCGGGGGCCGTGGCCACATTACCTCAGCGGGAGGGAGCAGAGCTGGGACAGGCAGCTAAAATGAGCAGCTCCCAAGAATCACTGCTCGACTCCCGGGGCCATTTGAAAGGAAACAATCCTTACGCAAAATCTTACACCCTGGTA
MWILALSLFQSFANVFSEDLHSSLYFVNASLQEVVFASTTGTLVPCPAAGIPPVTLRWYLATGEEIYDVPGIRHVHPNGTLQIFPFPPSSFSTLIHDNTYYCTAENPSGKIRSQDVHIKAVLREPYTVRVEDQKTMRGNVAVFKCIIPSSVEAYITVVSWEKDTVSLVSGSRFLITSTGALYIKDVQNEDGLYNYRCITRHRYTGETRQSNSARLFVSDPANSAPSILDGFDHRKAMAGQRVELPCKALGHPEPDYRWLKDNMPLELSGRFQKTVTGLLIENIRPSDSGSYVCEVSNRYGTAKVIGRLYVKQPLKATISPRKVKSSVGSQVSLSCSVTGTEDQELSWYRNGEILNPGKNVRITGINHENLIMDHMVKSDGGAYQCFVRKDKLSAQDYVQVVLEDGTPKIISAFSEKVVSPAEPVSLMCNVKGTPLPTITWTLDDDPILKGGSHRISQMITSEGNVVSYLNISSSQVRDGGVYRCTANNSAGVVLYQARINVRGPASIRPMKNITAIAGRDTYIHCRVIGYPYYSIKWYKNSNLLPFNHRQVAFENNGTLKLSDVQKEVDEGEYTCNVLVQPQLSTSQSVHVTVKVPPFIQPFEFPRFSIGQRVFIPCVVVSGDLPITITWQKDGRPIPGSLGVTIDNIDFTSSLRISNLSLMHNGNYTCIARNEAAAVEHQSQLIVRVPPKFVVQPRDQDGIYGKAVILNCSAEGYPVPTIVWKFSKGAGVPQFQPIALNGRIQVLSNGSLLIKHVVEEDSGYYLCKVSNDVGADVSKSMYLTVKIPAMITSYPNTTLATQGQKKEMSCTAHGEKPIIVRWEKEDRIINPEMARYLVSTKEVGEEVISTLQILPTVREDSGFFSCHAINSYGEDRGIIQLTVQEPPDPPEIEIKDVKARTITLRWTMGFDGNSPITGYDIECKNKSDSWDSAQRTKDVSPQLNSATIIDIHPSSTYSIRMYAKNRIGKSEPSNELTITADEAAPDGPPQEVHLEPISSQSIRVTWKAPKKHLQNGIIRGYQIGYREYSTGGNFQFNIISVDTSGDSEVYTLDNLNKFTQYGLVVQACNRAGTGPSSQEIITTTLEDVPSYPPENVQAIATSPESISISWSTLSKEALNGILQGFRVIYWANLMDGELGEIKNITTTQPSLELDGLEKYTNYSIQVLAFTRAGDGVRSEQIFTRTKEDVPGPPAGVKAAAASASMVFVSWLPPLKLNGIIRKYTVFCSHPYPTVISEFEASPDSFSYRIPNLSRNRQYSVWVVAVTSAGRGNSSEIITVEPLAKAPARILTFSGTVTTPWMKDIVLPCKAVGDPSPAVKWMKDSNGTPSLVTIDGRRSIFSNGSFIIRTVKAEDSGYYSCIANNNWGSDEIILNLQVQVPPDQPRLTVSKTTSSSITLSWLPGDNGGSSIRGYILQYSEDNSEQWGSFPISPSERSYRLENLKCGTWYKFTLTAQNGVGPGRISEIIEAKTLGKEPQFSKEQELFASINTTRVRLNLIGWNDGGCPITSFTLEYRPFGTTVWTTAQRTSLSKSYILYDLQEATWYELQMRVCNSAGCAEKQANFATLNYDGSTIPPLIKSVVQNEEGLTTNEGLKMLVTISCILVGVLLLFVLLLVVRRRRREQRLKRLRDAKSLAEMLMSKNTRTSDTLSKQQQTLRMHIDIPRAQLLIEERDTMETIDDRSTVLLTDADFGEAAKQKSLTVTHTVHYQSVSQATGPLVDVSDARPGTNPTTRRNAKAGPTARNRYASQWTLNRPHPTISAHTLTTDWRLPTPRAAGSVDKESDSYSVSPSQDTDRARSSMVSTESASSTYEELARAYEHAKMEEQLRHAKFTITECFISDTSSEQLTAGTNEYTDSLTSSTPSESGICRFTASPPKPQDGGRVMNMAVPKAHRPGDLIHLPPYLRMDFLLNRGGPGTSRDLSLGQACLEPQKSRTLKRPTVLEPIPMEAASSASSTREGQSWQPGAVATLPQREGAELGQAAKMSSSQESLLDSRGHLKGNNPYAKSYTLV
維真(zhen)生物(wu)的人(ren)源cDNA克隆(long)中,雖然我們大約70%的克隆(long)與RefSeq或GeneBank所發表的基(ji)因列序完全一致,但(dan)也有(you)些克隆(long)含(han)有(you)天(tian)然的(de)單核(he)苷酸多態性而包(bao)含(han)一至多個(ge)SNPs, 因此(ci)與RefSeq或GeneBank所發表的列(lie)序會(hui)有些不同。我們強(qiang)烈(lie)建議您在購買(mai)之(zhi)前仔細將我(wo)們克隆的序(xu)列(lie)(lie)與(yu)您所要求的序(xu)列(lie)(lie)進行(xing)對比,以(yi)決(jue)定所購買(mai)的克隆是否符(fu)合(he)您的要求。如果(guo)我們預制(zhi)的克隆不符(fu)合(he)您的要求,我們一般在1-2周內可以提供一個與您所要(yao)求的(de)序列完全一致(zhi)的(de)克隆。我(wo)們的(de)每一個克隆與RefSeq(2013)標準序列相比都(dou)含(han)有一(yi)個完整(zheng)的ORF(Opening Reading Frame)。因為(wei)RefSeq數據庫會不(bu)斷更(geng)新,更(geng)新后的標準序列將來有(you)可能與(yu)我們現有(you)的克隆(long)序列產生差異。我們所(suo)有(you)的克隆(long)產品都(dou)經過二代(NextGen)測序驗證,并且保證網站上克隆的序列與您收到(dao)的克隆序列完全(quan)一致。在(zai)未經(jing)維真生(sheng)物(wu)書面(mian)給予授權前,人源ORF cDNA克隆不(bu)可轉(zhuan)售(shou)或贈予買(mai)方(fang)以(yi)外的第三方(fang)。同時, 在使用(yong)任何維真產品之前,買(mai)方有(you)責任(ren)確(que)認該(gai)基(ji)因的使用(yong)(yong)不會(hui)侵犯到第三方的知識產權。此產品專門為基(ji)礎研究(jiu)所開發(fa),不適(shi)用(yong)(yong)于任(ren)何治療或(huo)診斷應用(yong)(yong)。