產品(pin)名稱:人源(yuan)cDNA克隆
產品規格:500μL甘油菌(jun)液
產品運輸條件:常溫
產品儲存條件和期限:-80℃長(chang)期保存
產(chan)品特點(dian):
? 人源基(ji)因(yin)C-端融合 Flag & 6×His 標簽;
? 含有(you)puromycin真(zhen)核抗性基因(yin),可篩選穩(wen)定(ding)轉染細胞株;
? 含有卡那霉素原核抗性基(ji)因,可篩選(xuan)重組克(ke)隆,推薦使用(yong)濃度為30μg/ml。
產品使用:
將甘油菌在(zai)新鮮(xian)配制(zhi)的固體(ti)培養(yang)基(相應(ying)抗性)中劃線,37℃培養箱(xiang)培養過(guo)(guo)夜(ye),挑取(qu)3個單(dan)克隆,分別(bie)接種于相應(ying)抗性的(de)液體培養基中(zhong),37℃搖床培養過(guo)(guo)夜(ye),然后進(jin)行質粒的(de)提取(qu)和驗(yan)證。
溫(wen)馨提示:考慮到實驗的嚴謹性,建議您拿到產品的第一時(shi)間進(jin)行測序確認(ren)。
ATGGCTACAGAAATTGGTTCTCCTCCTCGTTTTTTCCATATGCCAAGGTTCCAGCACCAGGCACCTCGACAGCTGTTTTATAAGCGACCTGATTTTGCACAACAGCAAGCAATGCAACAGCTTACTTTTGATGGAAAACGAATGAGAAAAGCTGTGAACCGAAAAACCATAGACTACAATCCATCTGTAATTAAGTATTTGGAGAACAGAATATGGCAAAGAGACCAGAGAGATATGCGGGCAATTCAGCCTGATGCAGGTTATTACAATGATCTGGTCCCACCTATAGGAATGTTGAATAATCCTATGAATGCAGTAACAACAAAATTTGTTCGGACATCAACAAATAAAGTAAAGTGTCCTGTATTTGTTGTTAGGTGGACTCCAGAAGGAAGACGCTTGGTCACTGGAGCTTCTAGTGGGGAGTTTACCCTGTGGAATGGACTCACTTTCAATTTTGAAACAATATTACAGGCTCACGACAGCCCAGTGAGGGCCATGACGTGGTCACATAATGACATGTGGATGTTGACAGCAGACCACGGAGGATATGTGAAATATTGGCAGTCGAACATGAACAACGTCAAGATGTTCCAGGCACATAAGGAGGCGATTAGAGAGGCCAGTTTCTCACCCACGGATAATAAATTTGCTACATGCTCTGATGACGGCACTGTTAGAATCTGGGACTTTCTTCGTTGCCATGAGGAAAGAATTCTCCGAGGGCATGGTGCTGATGTGAAATGTGTAGACTGGCATCCAACCAAAGGGTTAGTTGTTTCAGGAAGTAAAGATAGTCAACAGCCAATCAAGTTCTGGGATCCCAAGACTGGGCAGAGTCTTGCAACACTTCATGCCCATAAAAACACAGTAATGGAAGTGAAATTAAACCTCAATGGCAATTGGCTACTCACAGCATCACGTGATCATCTCTGTAAACTTTTTGATATCAGAAACCTAAAAGAAGAGCTTCAAGTCTTCCGAGGTCATAAGAAAGAAGCCACAGCTGTGGCCTGGCATCCTGTTCATGAAGGACTTTTTGCCAGTGGAGGGTCTGATGGTTCTTTGTTATTCTGGCATGTTGGGGTAGAGAAGGAAGTGGGTGGGATGGAGATGGCTCACGAAGGGATGATCTGGAGTCTGGCTTGGCATCCTCTTGGGCATATTCTCTGCTCAGGCTCAAATGACCATACTAGCAAATTCTGGACTCGAAACCGACCAGGTGATAAAATGCGAGATCGATATAATCTAAACCTTTTACCTGGAATGTCTGAAGATGGAGTAGAATATGATGACCTCGAACCTAATAGCCTGGCAGTAATTCCAGGAATGGGAATACCAGAACAACTAAAATTAGCTATGGAACAAGAACAGATGGGGAAAGATGAATCAAATGAAATTGAAATGACAATTCCAGGTTTAGATTGGGGAATGGAGGAAGTGATGCAAAAGGATCAGAAAAAAGTACCTCAGAAGAAAGTTCCTTATGCAAAACCCATTCCTGCTCAGTTCCAGCAGGCTTGGATGCAAAATAAAGTTCCAATTCCTGCTCCAAATGAGGTGCTGAATGACAGAAAAGAAGACATTAAATTGGAAGAGAAGAAAAAAACACAAGCAGAAATTGAGCAAGAAATGGCTACATTACAATATACTAACCCACAACTTCTGGAGCAACTTAAAATTGAAAGACTTGCACAGAAACAAGTTGAGCAAATTCAGCCTCCTCCCTCATCTGGCACCCCTCTCCTCGGACCCCAGCCTTTTCCAGGACAAGGTCCAATGTCTCAGATTCCTCAAGGTTTTCAACAGCCCCATCCATCTCAGCAGATGCCAATGAACATGGCTCAAATGGGGCCTCCAGGTCCACAGGGACAGTTTAGGCCTCCTGGACCCCAGGGACAAATGGGACCACAAGGTCCTCCACTGCATCAGGGAGGTGGGGGGCCACAAGGATTCATGGGACCACAGGGGCCCCAGGGCCCGCCCCAGGGGTTGCCACGGCCTCAGGACATGCATGGGCCCCAAGGAATGCAGAGGCATCCTGGACCTCATGGCCCTTTGGGACCTCAAGGGCCACCTGGACCACAAGGTAGTTCTGGTCCTCAAGGTCATATGGGTCCTCAGGGTCCACCTGGCCCACAGGGTCACATAGGCCCCCAAGGCCCGCCTGGCCCTCAGGGTCACTTGGGCCCACAGGGGCCTCCGGGTACTCAAGGTATGCAGGGACCACCTGGTCCCAGAGGAATGCAAGGGCCTCCTCATCCTCATGGGATCCAAGGCGGACCAGGGTCTCAAGGGATCCAAGGTCCTGTGTCTCAGGGACCTCTGATGGGATTGAATCCAAGAGGAATGCAGGGGCCTCCAGGCCCCCGGGAGAACCAGGGTCCTGCTCCCCAAGGGATGATTATGGGCCACCCGCCTCAAGAGATGAGAGGACCTCACCCTCCAGGTGGACTACTGGGACACGGCCCTCAGGAAATGAGAGGTCCTCAGGAGATCCGAGGCATGCAGGGGCCTCCACCCCAAGGATCAATGCTGGGACCTCCCCAGGAATTGCGAGGGCCTCCAGGCTCACAAAGTCAGCAGGGGCCGCCCCAGGGCTCTTTAGGACCTCCACCCCAGGGTGGCATGCAAGGACCCCCCGGACCTCAGGGACAGCAGAACCCAGCAAGAGGGCCACATCCATCTCAAGGGCCAATACCATTCCAGCAACAGAAAACGCCTCTGCTAGGTGATGGGCCCCGGGCCCCCTTCAACCAGGAAGGACAGAGCACAGGCCCCCCACCCCTGATACCAGGCCTAGGGCAGCAGGGAGCACAAGGTCGCATTCCCCCTCTGAACCCCGGACAAGGACCTGGCCCCAACAAAGGTGACTCCCGCGGCCCTCCAAACCATCACATGGGCCCGATGTCAGAGAGGCGGCATGAGCAGAGCGGCGGGCCTGAGCATGGGCCCGAGAGGGGGCCTTTCCGGGGTGGCCAGGACTGCAGGGGTCCCCCTGATAGGCGTGGCCCTCACCCAGACTTCCCCGATGACTTCAGCAGACCAGATGACTTCCACCCTGACAAGCGCTTTGGCCACCGGTTACGTGAATTTGAGGGGCGAGGAGGACCTTTACCGCAAGAAGAGAAGTGGAGGCGAGGGGGCCCTGGGCCTCCGTTTCCCCCTGATCACAGGGAATTCAGCGAGGGGGATGGCCGGGGTGCAGCCCGAGGCCCTCCGGGGGCATGGGAAGGCCGCAGGCCCGGAGATGAACGTTTCCCCCGGGATCCCGAGGACCCACGTTTTCGAGGGCGCAGAGAAGAAAGTTTCAGAAGAGGAGCCCCGCCGAGGCATGAGGGCCGTGCTCCCCCCAGAGGAAGGGATGGTTTTCCTGGTCCTGAAGACTTTGGTCCAGAGGAGAATTTTGATGCTTCTGAGGAAGCGGCCCGAGGACGAGATCTCAGAGGTCGAGGTCGGGGTACCCCACGAGGAGGAAGGAAGGGTTTACTTCCCACTCCTGACGAGTTCCCTCGCTTTGAAGGAGGGCGGAAGCCAGATTCCTGGGATGGAAACAGAGAGCCTGGGCCAGGTCATGAACATTTTCGTGATACTCCCCGCCCTGATCATCCCCCTCACGACGGTCATTCCCCAGCCAGCAGAGAACGCTCCTCTTCTCTCCAAGGCATGGACATGGCATCCCTACCTCCCCGAAAGCGCCCCTGGCATGATGGCCCAGGCACTTCTGAGCACAGAGAGATGGAGGCCCCAGGAGGCCCTTCTGAAGACCGAGGAGGCAAAGGCCGAGGGGGCCCAGGACCTGCTCAGAGAGTGCCCAAATCTGGGCGTTCCAGCTCCTTAGACGGAGAGCACCACGATGGATACCACAGAGATGAACCTTTTGGGGGCCCTCCAGGCAGTGGCACCCCTTCTCGAGGGGGCCGGAGTGGCAGTAACTGGGGTAGAGGGAGTAACATGAACTCTGGCCCGCCGAGGCGAGGAGCTTCACGGGGTGGTGGAAGGGGTCGG
MATEIGSPPRFFHMPRFQHQAPRQLFYKRPDFAQQQAMQQLTFDGKRMRKAVNRKTIDYNPSVIKYLENRIWQRDQRDMRAIQPDAGYYNDLVPPIGMLNNPMNAVTTKFVRTSTNKVKCPVFVVRWTPEGRRLVTGASSGEFTLWNGLTFNFETILQAHDSPVRAMTWSHNDMWMLTADHGGYVKYWQSNMNNVKMFQAHKEAIREASFSPTDNKFATCSDDGTVRIWDFLRCHEERILRGHGADVKCVDWHPTKGLVVSGSKDSQQPIKFWDPKTGQSLATLHAHKNTVMEVKLNLNGNWLLTASRDHLCKLFDIRNLKEELQVFRGHKKEATAVAWHPVHEGLFASGGSDGSLLFWHVGVEKEVGGMEMAHEGMIWSLAWHPLGHILCSGSNDHTSKFWTRNRPGDKMRDRYNLNLLPGMSEDGVEYDDLEPNSLAVIPGMGIPEQLKLAMEQEQMGKDESNEIEMTIPGLDWGMEEVMQKDQKKVPQKKVPYAKPIPAQFQQAWMQNKVPIPAPNEVLNDRKEDIKLEEKKKTQAEIEQEMATLQYTNPQLLEQLKIERLAQKQVEQIQPPPSSGTPLLGPQPFPGQGPMSQIPQGFQQPHPSQQMPMNMAQMGPPGPQGQFRPPGPQGQMGPQGPPLHQGGGGPQGFMGPQGPQGPPQGLPRPQDMHGPQGMQRHPGPHGPLGPQGPPGPQGSSGPQGHMGPQGPPGPQGHIGPQGPPGPQGHLGPQGPPGTQGMQGPPGPRGMQGPPHPHGIQGGPGSQGIQGPVSQGPLMGLNPRGMQGPPGPRENQGPAPQGMIMGHPPQEMRGPHPPGGLLGHGPQEMRGPQEIRGMQGPPPQGSMLGPPQELRGPPGSQSQQGPPQGSLGPPPQGGMQGPPGPQGQQNPARGPHPSQGPIPFQQQKTPLLGDGPRAPFNQEGQSTGPPPLIPGLGQQGAQGRIPPLNPGQGPGPNKGDSRGPPNHHMGPMSERRHEQSGGPEHGPERGPFRGGQDCRGPPDRRGPHPDFPDDFSRPDDFHPDKRFGHRLREFEGRGGPLPQEEKWRRGGPGPPFPPDHREFSEGDGRGAARGPPGAWEGRRPGDERFPRDPEDPRFRGRREESFRRGAPPRHEGRAPPRGRDGFPGPEDFGPEENFDASEEAARGRDLRGRGRGTPRGGRKGLLPTPDEFPRFEGGRKPDSWDGNREPGPGHEHFRDTPRPDHPPHDGHSPASRERSSSLQGMDMASLPPRKRPWHDGPGTSEHREMEAPGGPSEDRGGKGRGGPGPAQRVPKSGRSSSLDGEHHDGYHRDEPFGGPPGSGTPSRGGRSGSNWGRGSNMNSGPPRRGASRGGGRGR
維真生物的人源cDNA克隆中,雖(sui)然我們大(da)約70%的克隆與(yu)RefSeq或GeneBank所發表(biao)的基因列序完全一致,但也有些克(ke)隆含(han)有天(tian)然的單核苷酸多(duo)態性而包含(han)一至多(duo)個SNPs, 因此與RefSeq或(huo)GeneBank所發(fa)表的列序會有些不同。我們強烈(lie)建議您在購買之前仔細將我們克(ke)隆的序列(lie)與您(nin)所要求的序列(lie)進行對比,以決定(ding)所購買的克(ke)隆是(shi)否符合您的要(yao)求。如果我(wo)們預(yu)制的克(ke)隆不符合您的要(yao)求,我(wo)們一般在(zai)1-2周內(nei)可以提供一個(ge)與(yu)您(nin)所(suo)要求的(de)序列完全(quan)一致的(de)克(ke)隆(long)。我們的(de)每一個(ge)克(ke)隆(long)與(yu)RefSeq(2013)標準(zhun)序列相比(bi)都含有一個完整的ORF(Opening Reading Frame)。因為RefSeq數據庫會不斷更(geng)新,更(geng)新后(hou)的(de)標準序列(lie)將(jiang)來有(you)(you)可能與我(wo)們現有(you)(you)的(de)克隆(long)序列(lie)產生(sheng)差異。我(wo)們所有(you)(you)的(de)克隆(long)產品都經(jing)過二(er)代(NextGen)測序(xu)驗證,并且保證網站上克(ke)隆(long)的序(xu)列(lie)與您收到的克(ke)隆(long)序(xu)列(lie)完全(quan)一(yi)致。在未經(jing)維真生物書面(mian)給予授(shou)權前,人源ORF cDNA克隆不可轉售或贈予買(mai)方以外的(de)第三方。同時, 在(zai)使用(yong)任何維真產品之(zhi)前(qian),買方(fang)有責任(ren)確(que)認(ren)該基(ji)因的使用(yong)(yong)不會(hui)侵犯到第三方(fang)的知識(shi)產(chan)權。此產(chan)品專門(men)為基(ji)礎研究所(suo)開發,不適用(yong)(yong)于任(ren)何治療或診斷應(ying)用(yong)(yong)。