產(chan)品(pin)名稱:人(ren)源cDNA克(ke)隆(long)
產品規格:500μL甘油菌液
產品(pin)運輸條件:常溫
產品(pin)儲存條件和期限(xian):-80℃長期保存
產品特點:
? 人源基因C-端融合 Flag & 6×His 標簽;
? 含有(you)puromycin真核抗性(xing)基因,可篩選穩(wen)定轉(zhuan)染(ran)細胞(bao)株;
? 含有卡那霉素原核抗性基因,可(ke)篩選重組克(ke)隆,推薦使用濃度(du)為30μg/ml。
產(chan)品使用:
將甘油菌在新鮮(xian)配制的固體培養基(ji)(相應抗性)中劃(hua)線,37℃培養(yang)箱培養(yang)過夜(ye),挑(tiao)取(qu)3個單克隆,分別接種于相應抗性的(de)液(ye)體培養(yang)基(ji)中,37℃搖床培養(yang)過夜(ye),然(ran)后進行質粒(li)的(de)提取(qu)和驗證。
溫馨提(ti)示(shi):考(kao)慮到實驗的嚴謹性,建議您拿到產品(pin)的第一時(shi)間進行測序確(que)認(ren)。
CGCCATGGCGGTCGCCGTAAGAACTTTGCAGGAACAGCTGGAAAAGGCCAAAGAGAGTCTTAAGAACGTGGATGAGAACATTCGCAAGCTCACCGGGCGGGATCCGAATGACGTGAGGCCCATCCAAGCCAGATTGCTGGCCCTTTCTGGTCCTGGTGGAGGTAGAGGACGTGGTAGTTTATTACTGAGGCGTGGATTCTCAGATAGTGGAGGAGGACCCCCAGCCAAACAGAGAGACCTTGAAGGGGCAGTCAGTAGGCTGGGCGGGGAGCGTCGGACCAGAAGAGAATCACGCCAGGAAAGCGACCCGGAGGATGATGATGTTAAAAAGCCAGCATTGCAGTCTTCAGTTGTAGCTACCTCCAAAGAGCGCACACGTAGAGACCTTATCCAGGATCAAAATATGGATGAAAAGGGAAAGCAAAGGAACCGGCGAATATTTGGCTTGTTGATGGGTACCCTTCAAAAATTTAAACAAGAATCCACTGTTGCTACTGAAAGGCAAAAGCGGCGCCAGGAAATTGAACAAAAACTTGAAGTTCAGGCAGAAGAAGAGAGAAAGCAGGTTGAAAATGAAAGGAGAGAACTGTTTGAAGAGAGGCGTGCTAAACAGACAGAACTGCGGCTTTTGGAACAGAAAGTTGAGCTTGCGCAGCTGCAAGAAGAATGGAATGAACATAATGCCAAAATAATTAAATATATAAGAACTAAGACAAAGCCCCATTTGTTTTATATTCCTGGAAGAATGTGTCCAGCTACCCAAAAACTAATAGAAGAGTCACAGAGAAAAATGAACGCTTTATTTGAAGGTAGACGCATCGAATTTGCAGAACAAATAAATAAAATGGAGGCTAGGCCTAGAAGACAATCAATGAAGGAAAAAGAGCATCAGGTGGTGCGTAATGAAGAACAGAAGGCGGAACAAGAAGAGGGTAAGGTGGCTCAGCGAGAGGAAGAGTTGGAGGAGACAGGTAATCAGCACAATGATGTAGAAATAGAGGAAGCAGGAGAGGAAGAGGAAAAGGAAATAGCGATTGTTCATAGTGATGCAGAGAAAGAACAGGAGGAGGAAGAACAAAAACAGGAAATGGAGGTTAAGATGGAGGAGGAAACTGAGGTAAGGGAAAGTGAGAAGCAGCAGGATAGTCAGCCTGAAGAAGTTATGGATGTGCTAGAGATGGTTGAGAATGTCAAACATGTAATTGCTGACCAGGAGGTAATGGAAACTAATCGAGTTGAAAGTGTAGAACCTTCAGAAAATGAAGCTAGCAAAGAATTGGAACCAGAAATGGAATTTGAAATTGAGCCAGATAAAGAATGTAAATCCCTTTCTCCTGGGAAAGAGAATGTCAGTGCTTTAGACATGGAAAAGGAGTCTGAGGAAAAAGAAGAAAAAGAATCTGAGCCCCAACCTGAGCCTGTGGCTCAACCTCAGCCTCAGTCTCAGCCCCAGCTTCAGCTTCAATCCCAGTCCCAACCAGTACTCCAGTCCCAGCCTCCCTCTCAGCCTGAGGATTTGTCATTAGCTGTTTTACAGCCAACACCCCAAGTTACTCAGGAGCAAGGGCATTTACTACCTGAGAGGAAGGATTTTCCTGTAGAGTCTGTAAAACTCACTGAGGTACCAGTAGAGCCAGTCTTGACAGTACATCCAGAGAGCAAGAGCAAAACCAAAACTAGGAGCAGAAGTAGAGGTCGAGCTAGAAATAAAACAAGCAAGAGTAGAAGTCGAAGCAGTAGCAGTAGCAGTTCTAGTAGCAGTTCAACCAGTAGCAGCAGTGGAAGTAGTTCCAGCAGTGGAAGTACTAGCAGTCGCAGTAGTTCCAGTAGCAGCTCCAGTACAAGTGGCAGCAGCAGCAGAGATAGTAGCAGTAGCACTAGTAGTAGTAGTGAGAGTAGAAGTCGGAGTAGGGGCCGGGGACATAATAGAGATAGAAAGCACAGAAGGAGCGTGGATCGGAAGAGAAGGGATACTTCAGGACTAGAAAGAAGTCACAAATCTTCAAAAGGTGGTAGTAGTAGAGATACAAAAGGATCAAAGGATAAGAATTCCCGGTCCGACAGAAAGAGGTCTATATCAGAGAGTAGTCGATCAGGCAAAAGATCTTCAAGAAGTGAAAGAGACCGAAAATCAGACAGGAAAGACAAAAGGCGTA
MAVAVRTLQEQLEKAKESLKNVDENIRKLTGRDPNDVRPIQARLLALSGPGGGRGRGSLLLRRGFSDSGGGPPAKQRDLEGAVSRLGGERRTRRESRQESDPEDDDVKKPALQSSVVATSKERTRRDLIQDQNMDEKGKQRNRRIFGLLMGTLQKFKQESTVATERQKRRQEIEQKLEVQAEEERKQVENERRELFEERRAKQTELRLLEQKVELAQLQEEWNEHNAKIIKYIRTKTKPHLFYIPGRMCPATQKLIEESQRKMNALFEGRRIEFAEQINKMEARPRRQSMKEKEHQVVRNEEQKAEQEEGKVAQREEELEETGNQHNDVEIEEAGEEEEKEIAIVHSDAEKEQEEEEQKQEMEVKMEEETEVRESEKQQDSQPEEVMDVLEMVENVKHVIADQEVMETNRVESVEPSENEASKELEPEMEFEIEPDKECKSLSPGKENVSALDMEKESEEKEEKESEPQPEPVAQPQPQSQPQLQLQSQSQPVLQSQPPSQPEDLSLAVLQPTPQVTQEQGHLLPERKDFPVESVKLTEVPVEPVLTVHPESKSKTKTRSRSRGRARNKTSKSRSRSSSSSSSSSSSTSSSSGSSSSSGSTSSRSSSSSSSSTSGSSSRDSSSSTSSSSESRSRSRGRGHNRDRKHRRSVDRKRRDTSGLERSHKSSKGGSSRDTKGSKDKNSRSDRKRSISESSRSGKRSSRSERDRKSDRKDKRR
維真生(sheng)物的人源cDNA克(ke)隆中(zhong),雖然我(wo)們(men)大(da)約70%的克(ke)隆與RefSeq或GeneBank所發(fa)表的基(ji)因列序完全一致,但也有些(xie)克隆含(han)有天然(ran)的單核苷酸多(duo)態性而包含(han)一(yi)至(zhi)多(duo)個SNPs, 因此(ci)與RefSeq或GeneBank所發表的列序(xu)會有些(xie)不同。我們強(qiang)烈建議您在購買(mai)之前仔細將我(wo)們(men)克隆的序(xu)列與(yu)您所要求的序(xu)列進行對(dui)比,以(yi)決定(ding)所購買的(de)克隆是(shi)否符合您(nin)的(de)要求。如果我們(men)預制的(de)克隆不符合您(nin)的(de)要求,我們(men)一般(ban)在1-2周(zhou)內(nei)可以提供一個(ge)與您所要求的序列完全一致的克隆(long)。我們的每一個(ge)克隆(long)與RefSeq(2013)標準(zhun)序列(lie)相比都含有一(yi)個(ge)完(wan)整的ORF(Opening Reading Frame)。因(yin)為(wei)RefSeq數據庫(ku)會(hui)不斷更新(xin),更新(xin)后的(de)標準序列(lie)將來有可(ke)能與(yu)我(wo)們(men)(men)現有的(de)克(ke)隆序列(lie)產(chan)生(sheng)差(cha)異。我(wo)們(men)(men)所有的(de)克(ke)隆產(chan)品(pin)都(dou)經過二代(NextGen)測序(xu)驗證,并且保證網站上克(ke)隆的序(xu)列與(yu)您(nin)收(shou)到的克(ke)隆序(xu)列完全一致(zhi)。在未經(jing)維真生物書面給予授權前(qian),人源ORF cDNA克隆不可轉售或贈(zeng)予買方(fang)(fang)以外的第(di)三(san)方(fang)(fang)。同時(shi), 在使用任何維(wei)真產品(pin)之(zhi)前(qian),買方有責任確認該基因的使用不會(hui)侵犯到(dao)第三(san)方的知識(shi)產(chan)權。此產(chan)品專門(men)為基礎研究(jiu)所開發,不適用于任何治療或診斷應用。