產品名(ming)稱(cheng):人源cDNA克隆
產(chan)品規格:500μL甘油菌液
產品運輸條件:常溫(wen)
產品儲存條件(jian)和期限(xian):-80℃長期保存
產品特點:
? 人源基因C-端融合 Flag & 6×His 標簽;
? 含有puromycin真核抗性基因(yin),可(ke)篩選穩定轉染細胞株(zhu);
? 含(han)有卡那(nei)霉素(su)原核抗(kang)性基因,可篩選重組克隆(long),推薦使用濃度為30μg/ml。
產品使用(yong):
將甘油菌(jun)在(zai)新(xin)鮮配制的固體(ti)培(pei)養基(ji)(相應抗性)中劃線,37℃培養(yang)箱培養(yang)過夜,挑取3個單克隆,分別(bie)接種于相應抗性的液體(ti)培養(yang)基中,37℃搖床培養(yang)過夜,然后(hou)進(jin)行質粒的提(ti)取和(he)驗證。
溫馨提示:考慮(lv)到實(shi)驗的嚴謹性,建議您拿到產品的(de)第一(yi)時間(jian)進行測(ce)序確認。
CGCCATGGCGCTGCGGCCAGGAGCTGGTTCTGGTGGCGGCGGGGCCGCGGGAGCTGGCGCGGGGTCCGCCGGGGGAGGCGGCTTCATGTTTCCTGTTGCAGGTGGGATAAGACCCcctcaagcaggcctgatgCCGATGCAGCAACAAGGATTTCCTATGGTCTCTGTCATGCAGCCTAATATGCAAGGCATTATGGGAATGAATTACAGCTCTCAGATGTCCCAAGGACCTATTGCTATGCAGGCAGGAATACCAATGGGACCAATGCCAGCAGCGGGAATGCCTTacctaggacaagcACCCTTCCTGGGCATGCGTCCTCCAGGCCCACAGTACACTCCAGACATGCAGAAGCAGTTTGCCGAAGAGCAGCAGAAACGATTTGAACAGCAGCAAAAACTCTTAGAAGAAGAAAGAAAAAGACGCCAGTTTGAAGAGCAGAAGCAAAAGCTCAGACTTTTGAGCAGTGTGAAACCCAAGACAGGAGAGAAGAGTAGAGATGATGCTTTGGAAGCCATAAAAGGAAATTTAGATGGGTTTTCCAGAGATGCAAAAATGCACCCTACTCCAGCATCGCACCCCAAGAAACCAGGTGTGGGAGTATTTCCCTCACAGGATCCTGCTCAGCCCAGAATGCCTCCTTGGATTTACAATGAGAGTTTGGTTCCAGATGCCTATAAGAAAATCTTAGAAACCACAATGACTCCAACTGGAATAGATACTGCCAAACTGTATCCCATTCTGATGTCATCTGGGCTTCCCAGGGAAACTCTTGGACAGATATGGGCCTTAGCTAATCGAACTACACCTGGCAAACTTACAAAAGAAGAACTTTATACCGTTCTAGCCATGATAGCGGTAACACAGAGGGGCGTTCCTGCAATGAGTCCTGATGCTTTAAACCAGTTCCCAGCAGCTCCTATTCCAACTTTAAGTGGCTTTTCTATGACTCTGCCTACACCGGTGAGTCAGCCAACTGTGATACCTTCAGGTCCTGCGGGCTCCATGCCCCTCAGCCTTGGACAGCCAGTCATGGGCATTAACCTTGTTGGACCAGTGGGTGGAGCTGCAGCCCAGGCTTCTAGTGGTTTCATACCAACCTACCCTGCAAATCAGGTAGTAAAGCCAGAAGAAGATGACTTCCAGGATTTTCAAGATGCTTCTAAGTCAGGATCCCTTGATGACTCATTCAGTGATTTCCAAGAGTTGCCTGCTTCTTCAAAAACAAGTAACTCCCAGCATGGAAACAGTGCCCCTTCTTTGTTGATGCCACTTCCTGGAACTAAAGCATTGCCTTCAATGGACAAATATGCTGTGTTTAAAGGAATTGCAGCTGACAAGTCCTCTGAAAATACTGTTCCACCTGGAGATCCTGGTGATAAATATAGTGCTTTCAGAGAACTTGAACAGACAGCAGAGAATAAACCTTTAGGAGAAAGCTTTGCAGAATTCAGATCTGCAGGAACTGATGATGGTTTCACCGATTTTAAAACAGCCGATAGTGTATCACCACTAGAGCCACCAACAAAAGACAAAACTTTTCCACCATCCTTCCCCTCAGGAACTATACAACAGAAACAACAAACACAAGTGAAAAACCCTCTGAACTTAGCAGACCTAGATATGTTTTCCTCAGTTAATTGCAGCAGCGAGAAACCATTGTCTTTTTCAGCTGTGTTTAGCACATCAAAATCAGTTTCTACACCACAGTCAACAGGTTCTGCTGCTACTATGACAGCATTGGCAGCAACAAAAACTTCTAGTTTGGCTGATGATTTTGGAGAATTCAGCCTTTTTGGGGAATATTCTGGTCTAGCACCTGTTGGGGAGCAGGATGACTTTGCAGATTTTATGGCTTTCAGTAATAGCTCTATTTCATCTGAGCAAAAGCCGGATGACAAATATGATGCCCTTAAAGAGGAAGCCAGTCCTGTTCCTCTAACCAGCAACGTGGGCAGCACAGTGAAGGGTGGACAAAACTCGACTGCTGCGTCTACCAAGTACGATGTCTTCAGACAACTTTCTCTGGAAGGGTCTGGACTAGGTGTTGAAGACCTGAAAGATAACACTCCTTCAGGAAAAAGTGATGATGATTTTGCTGACTTCCACTCCAGTAAATTTTCTTCCATAAACTCGGACAAATCCCTGGGAGAGAAAGCAGTGGCTTTCAGACACACCAAAGAAGACTCTGCATCAGTGAAGTCCTTAGATCTCCCTTCCATTGGTGGCAGCAGTGTTGGCAAGGAGGACTCTGAAGATGCACTCTCTGTTCAGTTTGACATGAAATTGGCTGATGTGGGAGGAGATCTTAAGCATGTCATGTCTGATAGCTCTTTGGATTTACCAACAGTTAGTGGCCAGCATCCTCCTGCTGCAGATATAGAGGACTTAAAATATGCTGCTTTTGGAAGCTACAGTAGCAATTTTGCAGTGAGCACACTTACAAGCTATGACTGGTCagacagggatgatgCAACTCAGGGCAGAAAACTCTCTCCATTTGTCCTCTCAGCAGGAAGTGGATCCCCCTCAGCCACCTCAATTCTTCAAAAGAAAGAGACTTCATTTGGCAGTTCTGAAAACATCACCATGACATCTCTCTCCAAAGTAACGACCTTTGTAAGTGAAGATGCTCTTCCAGAGACCACCTTCCCAGCTCTTGCCAGTTTTAAAGACACGATTCCTCAGACCAGTGAGCAAAAGGAATATGAAAACAGAGACTATAAAGATTTCACAAAACAGGACCTGCCTACGGCTGAAcggagccaggaggccacgtGTCCCAGCCCAGCGTCCAGTGGTGCCTCTCAAGAAACCCCCAACGAATGTTCGGATGACTTTGGAGAGTTTCAAAGTGAAAAGCCCAAAATCAGCAAATTTGACTTCTTAGTAGCCACTTCACAAAGCAAAATGAAATCCAGTGAAGAAATGATCAAAAGTGAGCTGGCAACCTTTGACCTTTCTGTTCAAGGATCACACAAGAGGAGTTTGAGCCTTGGTGATAAAGAAATAAGCCGTTCTTCTCCTTCTCCAGCTTTGGAGCAGCCTTTCAGAGACCGTTCCAATACTCTGAATGAGAAGCCCGCCCTGCCCGTCATCCGAGACAAGTACAAAGACCTGACGGGAGAGGTGGAGGAAAATGAGAGATATGCATATGAATGGCAGAGATGCCTGGGGAGTGCCCTGAATGTCATTAAGAAGGCAAATGATACCTTAAATGGAATCAGTAGTAGTTCTGTTTGCACAGAAGTAATTCAGTCAGCTCAAGGCATGGAATATTTATTAGGTGTTGTTGAAGTGTACAGGGTAACCAAGCGTGTGGAGCTGGGGATAAAAGCCACTGCAGTGTGCAGTGAGAAACTCCAGCAGTTGCTGAAGGACATCGATAAAGTATGGAATAACCTAATCGGCTTCATGTCACTCGCCACACTCACatgctgctgggaaaagatgactgtgattacaaagcatctctctccttaccatgagctcttagaagaaaagCCAGATGAAAACTCGCTGGATTTTTCCTCCTGTATGTTACGGCCTGGGATTAAAAATGCTCAGGAGCTTGCCTGTGGAGTGTGCCTCTTGAATGTGGACTCGAGGAGCCGGAAAGAAGAGAAGCCTGCAGAAGAACATCCTAAAAAAGCATTCAACTCAGAAACAGACAGTTTCAAGCTGGCCTatggagggcaccagTATCACGCCAGCTGTGCCAACTTCTGGATCAACTGTGTCGAACCAAAGCCTCCTGGCCTCGTCCTGCCTGacctgctca
MALRPGAGSGGGGAAGAGAGSAGGGGFMFPVAGGIRPPQAGLMPMQQQGFPMVSVMQPNMQGIMGMNYSSQMSQGPIAMQAGIPMGPMPAAGMPYLGQAPFLGMRPPGPQYTPDMQKQFAEEQQKRFEQQQKLLEEERKRRQFEEQKQKLRLLSSVKPKTGEKSRDDALEAIKGNLDGFSRDAKMHPTPASHPKKPGVGVFPSQDPAQPRMPPWIYNESLVPDAYKKILETTMTPTGIDTAKLYPILMSSGLPRETLGQIWALANRTTPGKLTKEELYTVLAMIAVTQRGVPAMSPDALNQFPAAPIPTLSGFSMTLPTPVSQPTVIPSGPAGSMPLSLGQPVMGINLVGPVGGAAAQASSGFIPTYPANQVVKPEEDDFQDFQDASKSGSLDDSFSDFQELPASSKTSNSQHGNSAPSLLMPLPGTKALPSMDKYAVFKGIAADKSSENTVPPGDPGDKYSAFRELEQTAENKPLGESFAEFRSAGTDDGFTDFKTADSVSPLEPPTKDKTFPPSFPSGTIQQKQQTQVKNPLNLADLDMFSSVNCSSEKPLSFSAVFSTSKSVSTPQSTGSAATMTALAATKTSSLADDFGEFSLFGEYSGLAPVGEQDDFADFMAFSNSSISSEQKPDDKYDALKEEASPVPLTSNVGSTVKGGQNSTAASTKYDVFRQLSLEGSGLGVEDLKDNTPSGKSDDDFADFHSSKFSSINSDKSLGEKAVAFRHTKEDSASVKSLDLPSIGGSSVGKEDSEDALSVQFDMKLADVGGDLKHVMSDSSLDLPTVSGQHPPAADIEDLKYAAFGSYSSNFAVSTLTSYDWSDRDDATQGRKLSPFVLSAGSGSPSATSILQKKETSFGSSENITMTSLSKVTTFVSEDALPETTFPALASFKDTIPQTSEQKEYENRDYKDFTKQDLPTAERSQEATCPSPASSGASQETPNECSDDFGEFQSEKPKISKFDFLVATSQSKMKSSEEMIKSELATFDLSVQGSHKRSLSLGDKEISRSSPSPALEQPFRDRSNTLNEKPALPVIRDKYKDLTGEVEENERYAYEWQRCLGSALNVIKKANDTLNGISSSSVCTEVIQSAQGMEYLLGVVEVYRVTKRVELGIKATAVCSEKLQQLLKDIDKVWNNLIGFMSLATLTCCWEKMTVITKHLSPYHELLEEKPDENSLDFSSCMLRPGIKNAQELACGVCLLNVDSRSRKEEKPAEEHPKKAFNSETDSFKLAYGGHQYHASCANFWINCVEPKPPGLVLPDLL
維真生(sheng)物的人源cDNA克隆中,雖然我們大約70%的克隆與RefSeq或(huo)GeneBank所發表(biao)的基(ji)因列序(xu)完(wan)全一致,但也有些克隆含(han)有天然的單核苷酸多(duo)態性而包含(han)一至(zhi)多(duo)個SNPs, 因此與RefSeq或GeneBank所發表(biao)的列序會有些不同(tong)。我們強烈(lie)建議您在(zai)購買之前仔細將我們克隆的序列(lie)與您所要求的序列(lie)進行(xing)對比,以決定所購(gou)買的克隆是否(fou)符合您的要求(qiu)。如果我們(men)預制的克隆不符合您的要求(qiu),我們(men)一(yi)般在(zai)1-2周內可以提供一(yi)個(ge)與您(nin)所要求的(de)序列(lie)完全一(yi)致(zhi)的(de)克隆。我們(men)的(de)每一(yi)個(ge)克隆與RefSeq(2013)標(biao)準序列相(xiang)比都含有一(yi)個完整的(de)ORF(Opening Reading Frame)。因為RefSeq數據庫會不(bu)斷更新,更新后的標準序列(lie)將來有可能與我(wo)們(men)現(xian)有的克隆(long)序列(lie)產生差異(yi)。我(wo)們(men)所有的克隆(long)產品都經過二代(NextGen)測序驗證,并且保證網站上克隆的(de)序列與您(nin)收(shou)到的(de)克隆序列完(wan)全(quan)一致(zhi)。在未經維真生物(wu)書面給予授權前,人源ORF cDNA克隆(long)不可轉售或贈予買方(fang)(fang)以外的第(di)三方(fang)(fang)。同(tong)時, 在使用(yong)任何維(wei)真產品(pin)之前,買(mai)方有責任確認該基(ji)因的使(shi)用(yong)不會侵犯到第三方的知識產權(quan)。此產品專(zhuan)門為基(ji)礎研究所開發,不適用(yong)于任何(he)治療或診斷應(ying)用(yong)。