基因編號: NM_003253
產品編號: CH805399
基因名稱: TIAM1; FLJ36302
基因大小: 4776 bp
分子量: 175.12 kDa
酶切位點: SgfI_RsrII
價格: 7164
供貨時間: next day
產品名稱：人源cDNA克隆
產品規格：500μL甘油菌(jun)液
產品運輸條件：常溫
產(chan)品(pin)儲存條(tiao)件(jian)和期限：-80℃長期保存
產品特點：
? 人源基因C-端(duan)融(rong)合 Flag & 6×His 標(biao)簽；
? 含(han)有puromycin真核抗性基因，可(ke)篩選穩定(ding)轉染細(xi)胞株；
? 含有卡那霉素(su)原核(he)抗(kang)性(xing)基因，可(ke)篩選重組克(ke)隆，推(tui)薦(jian)使用濃(nong)度(du)為30μg/ml。
產品使用：
將甘油菌在(zai)新鮮(xian)配制的固體培養(yang)基（相應抗性）中(zhong)劃線，37℃培(pei)(pei)(pei)養(yang)箱培(pei)(pei)(pei)養(yang)過(guo)夜(ye)，挑取3個單克隆，分(fen)別接種于相應抗(kang)性(xing)的液(ye)體培(pei)(pei)(pei)養(yang)基中，37℃搖床培(pei)(pei)(pei)養(yang)過(guo)夜(ye)，然后進行(xing)質粒(li)的提(ti)取和(he)驗證。
溫馨提示(shi)：考慮到(dao)實驗(yan)的嚴謹性，建議您(nin)拿到產(chan)品(pin)的第一時間進行測序確認(ren)。
CGCCATGGGAAACGCAGAAAGTCAACATGTAGAGCACGAGTTTTATGGAGAAAAGCATGCCAGCCTGGGGCGCAAGCACACTTCCCGCTCCCTGCGCCTCTCGCACAAGACGCGGAGGACCAGGCACGCTTCCTCGGGGAAGGTGATCCACAGGAACTCCGAAGTGAGCACCCGATCCAGCAGCACCCCCAGCATCCCCCAGTCCCTGGCTGAAAATGGCCTGGAGCCCTTCTCCCAAGATGGTACCCTAGAAGACTTCGGGAGCCCCATCTGGGTGGACCGAGTGGACATGGGCTTGAGACCTGTGTCTTACACTGACTCTTCTGTCACTCCCAGCGTAGACAGCAGCATCGTCCTCACAGCAGCCTCTGTGCAGAGCATGCCAGACACTGAGGAGAGCAGGCTTTACGGGGATGACGCTACATATTTGGCTGAGGGAGGCAGGAGGCAGCATTCCTATACATCCAATGGGCCCACTTTCATGGAGACGGCGAGCTTTAAGAAGAAACGCTCCAAATCTGCAGACATCTGGCGGGAGGACAGCCTGGAATTCTCACTCTCTGATCTGAGCCAAGAACATTTAACAAGCAACGAAGAAATCTTGGGTTCCGCCGAAGAGAAGGACTGCGAGGAGGCTCGGGGGATGGAAACGCGGGCGAGTCCGCGGCAGCTCAGCACCTGTCAGAGAGCCAATTCCTTGGGTGACTTGTATGCTCAGAAAAACTCTGGAGTGACAGCAAACGGGGGGCCGGGGAGCAAATTTGCAGGCTACTGTCGGAATTTGGTGTCTGATATTCCCAATCTTGCAAACCATAAGATGCCACCAGCTGCTGCTGAAGAGACTCCTCCGTACAGTAATTATAACACACTTCCCTGTAGGAAATCTCACTGTCTCTCTGAAGGTGCCACCAACCCACAAATTAGCCATAGCAACAGCATGCAAGGCAGAAGAGCTAAAACAACTCAGGATGTTAATGCAGGCGAGGGCAGTGAGTTTGCAGACAGTGGGATTGAAGGGGCCACTACCGACACGGACCTCCTGTCCAGGCGATCTAATGCCACCAACTCCAGCTACTCACCCACCACAGGCCGGGCCTTTGTGGGCAGCGACAGCGGCAGCAGCTCCACCGGGGATGCGGCTCGTCAGGGGGTGTACGAGAACTTCCGGCGGGAGCTGGAGATGAGCACCACCAACAGCGAGAGCCTGGAGGAGGCCGGCTCGGCGCACAGCGATGAGCAGAGCAGCGGCACCCTGAGCTCTCCGGGCCAGTCGGACATCCTGCTGACCGCCGCACAGGGCACGGTGCGCAAGGCCGGCGCCCTGGCCGTCAAGAACTTCCTGGTGCACAAGAAGAACAAGAAGGTGGAGTCAGCCACCCGGAGGAAGTGGAAGCACTACTGGGTGTCCCTGAAAGGATGCACGCTATTTTTCTACGAGAGCGACGGCAGGTCTGGGATAGACCACAACAGCATCCCCAAACACGCCGTCTGGGTGGAGAACAGCATTGTGCAGGCGGTGCCTGAGCACCCCAAGAAGGACTTTGTCTTCTGCCTCAGCAATTCCCTGGGTGATGCCTTCCTTTTTCAGACCACTAGCCAGACGGAGCTTGAAAACTGGATCACCGCCATCCACTCTGCCTGCGCCACTGCGGTCGCGAGGCACCACCACAAGGAAGACACGCTCCGACTCCTGAAATCAGAGATCAAAAAACTGGAACAGAAGATTGACATGGATGAAAAGATGAAGAAAATGGGTGAAATGCAGCTGTCTTCAGTCACTGACTCAAAGAAAAAGAAAACAATATTAGATCAGATCTTTGTCTGGGAGCAAAATCTCGAGCAGTTCCAAATGGACCTGTTTCGTTTCCGCTGTTATTTAGCCAGCCTTCAGGGTGGGGAGCTGCCAAACCCCAAAAGGCTTCTCGCTTTTGCAAGTCGACCAACGAAAGTGGCCATGGGCCGCCTTGGAATCTTTTCGGTATCATCGTTTCATGCCCTGGTGGCAGCACGCACTGGTGAAACTGGAGTGAGAAGACGTACTCAGGCCATGTCCAGATCCGCGAGCAAGCGAAGGAGCAGGTTTTCTTCTCTGTGGGGTCTGGATACTACCTCCAAAAAGAAGCAGGGACGGCCAAGCATCAATCAGGTGTTTGGAGAGGGAACCGAAGCTGTAAAGAAATCTTTAGAGGGAATATTTGATGACATTGTTCCAGATGGCAAGAGGGAGAAAGAAGTGGTCTTACCTAACGTTCACCAGCACAACCCTGACTGCGACATTTGGGTCCACGAGTATTTCACTCCATCCTGGTTCTGTCTGCCCAATAATCAGCCTGCCCTGACGGTCGTCCGGCCAGGCGACACTGCACGGGACACCCTGGAGCTGATTTGCAAGACACATCAACTGGATCATTCTGCTCATTACCTGCGCCTGAAATTTCTAATAGAAAACAAAATGCAGCTCTATGTTCCACAGCCCGAGGAAGACATCTATGAGCTGCTGTACAAAGAAATTGAAATCTGTCCAAAAGTCACTCAGAGCATCCACATTGAGAAGTCAGATACAGCTGCTGATACTTACGGGTTTTCACTTTCTTCTGTGGAAGAAGATGGTATTCGAAGGCTGTACGTGAATAGTGTGAAGGAAACCGGTTTAGCTTCCAAGAAAGGCCTGAAAGCAGGAGATGAGATTCTTGAGATCAATAATCGTGCTGCTGACGCCCTGAACTCTTCTATGCTCAAAGATTTCCTCTCACAGCCCTCGCTGGGCCTCCTGGTGAGGACCTACCCCGAGCTGGAGGAAGGAGTGGAGCTGCTGGAAAGCCCGCCCCACCGAGTGGACGGCCCTGCCGACCTTGGCGAGAGCCCCCTCGCCTTTCTCACCAGCAACCCAGGGCACAGCCTTTGCAGCGAGCAGGGCAGCAGTGCTGAGACCGCTCCAGAGGAGACCGAGGGGCCAGACTTGGAATCCTCAGATGAGACTGATCACAGCAGCAAGAGTACAGAACAGGTGGCCGCATTTTGCCGCAGTTTGCATGAGATGAACCCCTCTGACCAGAGCCCATCTCCTCAGGACTCCACGGGGCCTCAGCTGGCGACCATGAGACAACTCTCGGATGCAGATAAGCTGCGCAAGGTGATCTGCGAGCTCCTGGAGACGGAGCGCACCTACGTGAAGGATTTAAACTGTCTTATGGAGAGATACCTAAAGCCTCTTCAAAAAGAAACTTTTCTCACCCAGGATGAGCTTGACGTGCTTTTTGGAAATTTAACGGAAATGGTAGAGTTTCAAGTAGAATTCCTTAAAACTCTAGAAGATGGAGTGAGACTGGTACCTGATTTGGAAAAGCTTGAGAAGGTTGATCAATTTAAGAAAGTGCTGTTCTCTCTGGGGGGATCATTCCTGTATTATGCTGACCGCTTCAAGCTCTACAGTGCCTTCTGCGCCAGCCACACAAAAGTTCCCAAGGTCCTGGTGAAAGCCAAGACAGACACGGCTTTCAAGGCATTCTTGGATGCCCAGAACCCGAAGCAGCAGCACTCATCCACGCTGGAGTCGTACCTCATCAAGCCCATCCAGAGGATCCTCAAGTACCCACTTCTGCTCAGGGAGCTGTTCGCCCTGACCGATGCGGAGAGCGAGGAGCACTACCACCTGGACGTGGCCATCAAGACCATGAACAAGGTTGCCAGTCACATCAATGAGATGCAGAAAATCCATGAAGAGTTTGGGGCTGTGTTTGACCAGCTGATTGCTGAACAGACTGGTGAGAAAAAAGAGGTTGCAGATCTGAGCATGGGAGACCTGCTTTTGCACACTACCGTGATCTGGCTGAACCCGCCGGCCTCGCTGGGCAAGTGGAAAAAGGAACCAGAGTTGGCAGCATTCGTCTTCAAAACTGCTGTGGTCCTTGTGTATAAAGATGGTTCCAAACAGAAGAAGAAACTTGTAGGATCTCACAGGCTTTCCACTTATGAGGACTGGGACCCCTTCAGATTTCGACACATGATCCCCACGGAAGCGCTGCAGGTTCGAGCTTTGGCGAGTGCAGATGCAGAGGCAAATGCCGTGTGTGAAATTGTCCATGTAAAATCCGAGTCTGAAGGGAGGCCGGAGAGGGTCTTTCACTTGTGCTGCAGCTCCCCAGAGAGCCGAAAGGATTTCCTAAAGGCTGTGCATTCAATCCTGCGTGATAAGCACAGAAGACAGCTCCTCAAAACCGAGAGCCTTCCCTCATCCCAGCAATATGTCCCTTTTGGAGGCAAAAGATTGTGTGCACTGAAGGGGGCCAGGCCGGCCATGAGCAGGGCAGTGTCTGCCCCAAGCAAGTCTCTTGGGAGGAGGAGGCGGCGGCTGGCTCGAAACAGGTTTACCATTGATTCTGATGCCGTCTCCGCAAGCAGCCCGGAGAAAGAGTCCCAGCAGCCCCCCGGTGGTGGGGACACTGACCGATGGGTAGAGGAGCAGTTTGATCTTGCTCAGTATGAGGAGCAAGATGACATCAAGGAGACAGACATCCTCAGTGACGATGATGAGTTCTGTGAGTCCGTGAAGGGTGCCTCAGTGGACAGAGACCTGCAGGAGCGGCTTCAGGCCACCTCCATCAGTCAGCGGGAAAGAGGCCGGAAAACCCTGGATAGTCACGCGTCCCGCATGGCACAGCTCAAGAAGCAAGCTGCCCTGTCGGGGATCAATGGAGGCCTGGAGAGCGCAAGCGAGGAAGTCATTTGGGTTAGGCGTGAAGACTTTGCCCCCTCCAGGAAACTGAACActgagatca
MGNAESQHVEHEFYGEKHASLGRKHTSRSLRLSHKTRRTRHASSGKVIHRNSEVSTRSSSTPSIPQSLAENGLEPFSQDGTLEDFGSPIWVDRVDMGLRPVSYTDSSVTPSVDSSIVLTAASVQSMPDTEESRLYGDDATYLAEGGRRQHSYTSNGPTFMETASFKKKRSKSADIWREDSLEFSLSDLSQEHLTSNEEILGSAEEKDCEEARGMETRASPRQLSTCQRANSLGDLYAQKNSGVTANGGPGSKFAGYCRNLVSDIPNLANHKMPPAAAEETPPYSNYNTLPCRKSHCLSEGATNPQISHSNSMQGRRAKTTQDVNAGEGSEFADSGIEGATTDTDLLSRRSNATNSSYSPTTGRAFVGSDSGSSSTGDAARQGVYENFRRELEMSTTNSESLEEAGSAHSDEQSSGTLSSPGQSDILLTAAQGTVRKAGALAVKNFLVHKKNKKVESATRRKWKHYWVSLKGCTLFFYESDGRSGIDHNSIPKHAVWVENSIVQAVPEHPKKDFVFCLSNSLGDAFLFQTTSQTELENWITAIHSACATAVARHHHKEDTLRLLKSEIKKLEQKIDMDEKMKKMGEMQLSSVTDSKKKKTILDQIFVWEQNLEQFQMDLFRFRCYLASLQGGELPNPKRLLAFASRPTKVAMGRLGIFSVSSFHALVAARTGETGVRRRTQAMSRSASKRRSRFSSLWGLDTTSKKKQGRPSINQVFGEGTEAVKKSLEGIFDDIVPDGKREKEVVLPNVHQHNPDCDIWVHEYFTPSWFCLPNNQPALTVVRPGDTARDTLELICKTHQLDHSAHYLRLKFLIENKMQLYVPQPEEDIYELLYKEIEICPKVTQSIHIEKSDTAADTYGFSLSSVEEDGIRRLYVNSVKETGLASKKGLKAGDEILEINNRAADALNSSMLKDFLSQPSLGLLVRTYPELEEGVELLESPPHRVDGPADLGESPLAFLTSNPGHSLCSEQGSSAETAPEETEGPDLESSDETDHSSKSTEQVAAFCRSLHEMNPSDQSPSPQDSTGPQLATMRQLSDADKLRKVICELLETERTYVKDLNCLMERYLKPLQKETFLTQDELDVLFGNLTEMVEFQVEFLKTLEDGVRLVPDLEKLEKVDQFKKVLFSLGGSFLYYADRFKLYSAFCASHTKVPKVLVKAKTDTAFKAFLDAQNPKQQHSSTLESYLIKPIQRILKYPLLLRELFALTDAESEEHYHLDVAIKTMNKVASHINEMQKIHEEFGAVFDQLIAEQTGEKKEVADLSMGDLLLHTTVIWLNPPASLGKWKKEPELAAFVFKTAVVLVYKDGSKQKKKLVGSHRLSTYEDWDPFRFRHMIPTEALQVRALASADAEANAVCEIVHVKSESEGRPERVFHLCCSSPESRKDFLKAVHSILRDKHRRQLLKTESLPSSQQYVPFGGKRLCALKGARPAMSRAVSAPSKSLGRRRRRLARNRFTIDSDAVSASSPEKESQQPPGGGDTDRWVEEQFDLAQYEEQDDIKETDILSDDDEFCESVKGASVDRDLQERLQATSISQRERGRKTLDSHASRMAQLKKQAALSGINGGLESASEEVIWVRREDFAPSRKLNTEI
維真生(sheng)物的(de)人源cDNA克(ke)隆中，雖然我們大約70%的(de)克隆與(yu)RefSeq或(huo)GeneBank所發表的基因列(lie)序(xu)完全一致,但也有些克(ke)隆含有天然的單核苷酸多態性而包含一(yi)至(zhi)多個SNPs, 因此與RefSeq或(huo)GeneBank所發表(biao)的列(lie)序(xu)會(hui)有些不同。我(wo)們強烈建議(yi)您在購買之前仔細將我(wo)們克隆的(de)序(xu)列(lie)與(yu)您所要求的(de)序(xu)列(lie)進行(xing)對比(bi)，以(yi)決定所(suo)購買的(de)克隆是(shi)否(fou)符合(he)您的(de)要(yao)求(qiu)。如果我們(men)預制的(de)克隆不符合(he)您的(de)要(yao)求(qiu)，我們(men)一般在1-2周內可以提供一個與您所要(yao)求(qiu)的序列完全一致的克隆(long)(long)。我們的每(mei)一個克隆(long)(long)與RefSeq（2013）標準序列相比都含有一個完(wan)整的ORF(Opening Reading Frame)。因為RefSeq數據庫會不斷更新，更新后的標準序(xu)列將來有(you)可能與我(wo)們(men)現有(you)的克隆序(xu)列產生(sheng)差異。我(wo)們(men)所(suo)有(you)的克隆產品都經過二代(dai)（NextGen）測(ce)序(xu)驗證，并且(qie)保證網站上(shang)克(ke)隆的序(xu)列與您(nin)收(shou)到的克(ke)隆序(xu)列完全(quan)一致。在(zai)未經(jing)維(wei)真(zhen)生物(wu)書面(mian)給予(yu)授權前，人源ORF cDNA克隆不(bu)可轉售(shou)或贈予買方(fang)以外的(de)第三方(fang)。同時(shi), 在(zai)使用(yong)任何維真產品之前(qian)，買方(fang)有責任(ren)確(que)認該基(ji)因(yin)的使(shi)用不會侵犯到第(di)三方(fang)的知(zhi)識產權。此產品專門為基(ji)礎研(yan)究所(suo)開發(fa)，不適用于任(ren)何治(zhi)療(liao)或診斷(duan)應(ying)用。
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