產品(pin)名(ming)稱:人源cDNA克隆
產品規格(ge):500μL甘(gan)油(you)菌液
產品運輸(shu)條件:常溫
產(chan)品(pin)儲(chu)存條(tiao)件和(he)期限:-80℃長期保存
產(chan)品(pin)特點(dian):
? 人源基因(yin)C-端(duan)融合 Flag & 6×His 標簽;
? 含有(you)puromycin真(zhen)核抗性基因,可篩選穩(wen)定轉染細胞株(zhu);
? 含有卡那霉素原核抗性基因,可(ke)篩選重組克隆,推薦使用濃(nong)度(du)為30μg/ml。
產品使用:
將甘油菌在新鮮配(pei)制的固體培養(yang)基(相應抗性)中劃線,37℃培養箱培養過夜,挑取(qu)3個單克隆,分(fen)別接種(zhong)于相應抗(kang)性(xing)的(de)(de)液體培養基中,37℃搖床培養過夜,然后進行質粒的(de)(de)提(ti)取(qu)和驗證。
溫(wen)馨(xin)提示:考慮到(dao)實驗的嚴(yan)謹性,建議您拿(na)到(dao)產品(pin)的第(di)一時間(jian)進行(xing)測序確認(ren)。
ATGGAAGGGGCGCTGACTGCCCGGGACAAGGTCGGGGTTCAGGATTTTGTGCTATTGGACGCGTACACCAGCGAATCTGCCTTTGTCGACAACCTCCGCAAGCGTTTCAGCGAGAACCTCATATACACATATATTGGCACCCTCCTTGTGTCTGTGAATCCATACCAGGAGCTCGGAATCTACACTGTGAGCCAGATGGAACTTTATCAAGGGGTCAATTTCTTTGAACTGCCACCACATGTCTACGCTATAGCCGACAACGCTTACCGAATGATGTGTGCTGAACTAAATAACCATTTCATCCTCATTTCTGGAGAGAGTGGGGCAGGGAAAACAGAGGCCTCCAAGAAAATTCTCGAGTATTTTGCAGTGACCTGCCCAATGACCCAGTCACTACAAATAGCCCGTGACAGACTGCTGTTCTCCAACCCAGTGCTGGAGGCTTTTGGAAATGCCAGAACGCTCCGGAATGACAACTCCAGCAGATTTGGGAAATACATGGATATACAATTTGATTTTCAGGGCATTCCCGTAGGTGGGCATATCATCAGTTACTTGATAGAGAAGTCCCGAGTTGTCTACCAAAACGAAGGCGAGCGGAATTTCCACATCTTCTACCAGCTGCTGGCAGGTGGCGAAGAGGAGCGCCTGTCTTACCTGGGACTCGAGCGAGACCCCCAGCTGTATAAATACCTCTCACAGGGTCATTGTGCCAAAGAGTCATCCATTAGTGACAAGAATGACTGGAAAACTGTTTCCAACGCCTTTTCTGTCATTGATTTTACTGAAGCTGACCTCGAGAATCTCTTTGGAATTATTGCCAGTGTCTTACACCTGGGGAACATTGGTTTTGAAGAAGACGACCAAGGCTGTGCCACTATCCCAGACACCCATGAGATCAAGTGGATAGCCAAGCTCCTGGGGGTCCACCCATCAGTCCTTCTGGAAGCTCTCACCCACAGAAAAATTGAAGCCAAAACTGAGGAGGTGATCTGCCCGTTGACACTAGAACTCTCTGTCTACGCTAGAGATGCAATGGCAAAGGCTGTTTATGGACGAACGTTTACTTGGCTGGTCAACAAAATCAATTCCTCCTTAGTTAACAAGGATTTCACCAGGAAAACTGTAATTGGATTACTGGACATCTATGGGTTTGAAGTCTTTGACAAGAATGGTTTTGAACAGTTCTGTATAAATTACTGCAATGAGAAACTCCAGCAACTGTTAATTGAGAGGACTCTAAAAGCAGAACAGGCAGAATATGAAATGGAAGGCATAGAGTGGGAGCCAATTAAATATTTCAACAACAAGATCATCTGTGATTTGGTAGAAGAGAGACATAAAGGAATCATATCTATTCTGGATGAAGAATGCATTCGGCCTGGTCCTGCTACAGACTTGAGTTTCCTGGAGAAATTGGAAGAGAAAGTGGGCAAACATGCACACTTCGAAACCCGTAAGCTGGCTGGTCCAAAGGGCCGAAAGAGGATTGGCTGGATGGAGTTCCGACTCCTCCACTATGCAGGAGAGGTCACATACTGCACCAAGGGATTCTTGGAAAAAAACAATGATCTTCTTTACAGACATCTGAAAGAAGTGCTGTGCAAGTCCAAGAACATTATCCTGAGGGAATGCTTCCTGCTGGCCGAGTTAGAAAACCGGAGGAGGCCCCCAACAGTGGGGACTCAGTTTAAAAACAGTCTGAGCAGCCTTCTAGAAACCCTCATCTCTAAGGAGCCCTCCTACATCCGTTGCATCAAGCCCAACGACAGGAAAGAACCCAGCAAATTTGATGACTTCCTCATAAGGCATCAGATCAAATACCTGGGGCTGATGGAGCACCTGCGGGTGAGACGGGCTGGTTTTGCATACCGAAGGAAATACGAGCATTTCTTGCAAAGGTACAAGTCCTTATGCCCAGACACCTGGCCGCACTGGCACGGGCCTCCAGCAGAGGGCGTGGAACGGCTGATCAAGTACATCGGCTACAAACCCGAGGAATACAAGTTAGGCAAAACCAAAATATTCATTCGTTTCCCCAGAACTCTGTTTGCTACCGAAGATGCCTTTGAATTTAGTAAACATCAACTAGTTGCAAGAATCCAAGCCACTTACAAACGCTGCCTAGGAAGGAGAGAATACGTGAAAAAAAGACAAGCAGCCATCAAACTGGAAGCCCACTGGCGTGGGGCCCTGGCTCGGAAGGCAATCCAAAGGAGAAAGTGGGCCGTGCGGATTATCAGAAAGTTCATTAAAGGATTCATCAGTCGCAACAAACCCCTCTGTCCTGACAACGAGGAATTTATCGTGTTTGTGCGGAAGAATTACATCTTGAATCTCCGGTATCACCTTCCAAAGACTGTCCTGGACAAGAGCTGGCTGAGGCCTCCTGGCATCTTGGAAAATGCATCAGATCTGCTCAGGAAAATGTGCGTGAGGAACCTGGTGCAGAAGTACTGCCGCGGGATCACAGCTGAGCGGAAAGCAATGATGCAGCAAAAGGTAGTTACAAGTGAAATCTTCAGGGGAAGGAAAGACGGCTACACAGAAAGTTTAAATCAACCCTTTGTCAACAGTCGGATAGATGAAGGAGACATTAATCCGAAAGTGCTTCAGCTAATTAGCCATGAGAAAATCCAGTATGGTGTCCCGGTCATTAAATATGACAGAAAAGGCTTCAAAGCACGGCAGCGGCAACTCATTCTTACTCAGAAAGCAGCTTACGTGGTGGAACTTGCCAAAATCAAGCAGAAAATAGAGTACTCAGCTCTCAAAGGTGTCTCCACTAGCAATCTGAGTGATGGAATCTTAGTCATTCATGTTTCACCAGAGGACAGCAAGCAAAAGGGGGATGCCGTTTTGCAGTGTGGACACGTGTTTGAAGCAGTTACTAAACTCGTCATGCTGGTTAAGAAGGAGAACATTGTCAATGTTGTTCAAGGAAGTTTACAGTTTTTTATTAGTCCGGGAAAAGAAGGCACAATAGTTTTCGACACTGGACTGGAAGAACAAGTCTATAAAAATAAAAATGGACAATTAACAGTGGTGTCAGTCCGGAGGAAGTCC
MEGALTARDKVGVQDFVLLDAYTSESAFVDNLRKRFSENLIYTYIGTLLVSVNPYQELGIYTVSQMELYQGVNFFELPPHVYAIADNAYRMMCAELNNHFILISGESGAGKTEASKKILEYFAVTCPMTQSLQIARDRLLFSNPVLEAFGNARTLRNDNSSRFGKYMDIQFDFQGIPVGGHIISYLIEKSRVVYQNEGERNFHIFYQLLAGGEEERLSYLGLERDPQLYKYLSQGHCAKESSISDKNDWKTVSNAFSVIDFTEADLENLFGIIASVLHLGNIGFEEDDQGCATIPDTHEIKWIAKLLGVHPSVLLEALTHRKIEAKTEEVICPLTLELSVYARDAMAKAVYGRTFTWLVNKINSSLVNKDFTRKTVIGLLDIYGFEVFDKNGFEQFCINYCNEKLQQLLIERTLKAEQAEYEMEGIEWEPIKYFNNKIICDLVEERHKGIISILDEECIRPGPATDLSFLEKLEEKVGKHAHFETRKLAGPKGRKRIGWMEFRLLHYAGEVTYCTKGFLEKNNDLLYRHLKEVLCKSKNIILRECFLLAELENRRRPPTVGTQFKNSLSSLLETLISKEPSYIRCIKPNDRKEPSKFDDFLIRHQIKYLGLMEHLRVRRAGFAYRRKYEHFLQRYKSLCPDTWPHWHGPPAEGVERLIKYIGYKPEEYKLGKTKIFIRFPRTLFATEDAFEFSKHQLVARIQATYKRCLGRREYVKKRQAAIKLEAHWRGALARKAIQRRKWAVRIIRKFIKGFISRNKPLCPDNEEFIVFVRKNYILNLRYHLPKTVLDKSWLRPPGILENASDLLRKMCVRNLVQKYCRGITAERKAMMQQKVVTSEIFRGRKDGYTESLNQPFVNSRIDEGDINPKVLQLISHEKIQYGVPVIKYDRKGFKARQRQLILTQKAAYVVELAKIKQKIEYSALKGVSTSNLSDGILVIHVSPEDSKQKGDAVLQCGHVFEAVTKLVMLVKKENIVNVVQGSLQFFISPGKEGTIVFDTGLEEQVYKNKNGQLTVVSVRRKS
維真生(sheng)物的(de)人源cDNA克隆中(zhong),雖然我們大約70%的克隆與(yu)RefSeq或GeneBank所(suo)發表的基因列序完全一致,但(dan)也有(you)些克隆含(han)有(you)天然的單核苷酸多態性而包含(han)一(yi)至(zhi)多個SNPs, 因此與(yu)RefSeq或GeneBank所(suo)發表(biao)的列序會有些不同。我們強烈建(jian)議(yi)您在購(gou)買之前仔細將我們克隆的序列與您所要求的序列進行對比,以決定所(suo)購買的克隆是(shi)否符合您(nin)的要(yao)求。如果(guo)我(wo)們預制(zhi)的克隆不符合您(nin)的要(yao)求,我(wo)們一(yi)般在1-2周內可以提供一(yi)個(ge)(ge)與(yu)您所要求(qiu)的(de)序列完全一(yi)致的(de)克隆。我們的(de)每一(yi)個(ge)(ge)克隆與(yu)RefSeq(2013)標準序列相(xiang)比(bi)都含有一個完整的ORF(Opening Reading Frame)。因為RefSeq數(shu)據庫會不斷更新(xin),更新(xin)后(hou)的(de)標準(zhun)序列將來有(you)可能與(yu)我(wo)們現有(you)的(de)克隆(long)序列產(chan)(chan)生(sheng)差異。我(wo)們所有(you)的(de)克隆(long)產(chan)(chan)品都經過二(er)代(NextGen)測(ce)序驗證,并(bing)且(qie)保證網站(zhan)上克(ke)(ke)隆(long)的序列與您收到的克(ke)(ke)隆(long)序列完全一致。在未經維真生物書面給予(yu)授權前,人源ORF cDNA克隆不可轉售或(huo)贈予買方以外的第三方。同時, 在使用(yong)任何維真產品之前,買方有(you)責(ze)任(ren)確認該基(ji)因的(de)使用不(bu)會(hui)侵犯到第(di)三方的(de)知識產(chan)(chan)權。此產(chan)(chan)品專門為基(ji)礎研究所開發(fa),不(bu)適用于任(ren)何治療或診斷(duan)應用。