產品名稱:人源cDNA克隆
產品(pin)規格(ge):500μL甘油菌液(ye)
產品運輸條件:常溫
產品(pin)儲存條件和期限:-80℃長(chang)期保存
產品特點:
? 人源基(ji)因(yin)C-端融合 Flag & 6×His 標(biao)簽;
? 含有puromycin真核抗性基因,可篩選穩定轉染細胞株;
? 含(han)有(you)卡那(nei)霉素(su)原(yuan)核抗(kang)性基(ji)因,可篩選重組克(ke)隆,推薦使用濃(nong)度為30μg/ml。
產品使用:
將甘油菌在新鮮配(pei)制的(de)固(gu)體(ti)培(pei)養基(相應抗性)中(zhong)劃線,37℃培(pei)(pei)養(yang)箱培(pei)(pei)養(yang)過(guo)夜(ye),挑取3個單克隆,分別接(jie)種于相應(ying)抗性(xing)的(de)液體培(pei)(pei)養(yang)基中,37℃搖床培(pei)(pei)養(yang)過(guo)夜(ye),然后(hou)進行質(zhi)粒(li)的(de)提(ti)取和驗證。
溫馨提示(shi):考慮到實驗的嚴謹(jin)性,建議您拿到產品的第一時(shi)間進行測序確認。
ATGGAGAATCTTCCCTTTCCTTTAAAATTACTTAGTGCCTCATCGCTAAACGCCCCCAGCTCCACACCATGGGTGTTGGATATCTTCCTCACCTTGGTGTTTGCCCTGGGGTTCTTCTTCCTATTACTCCCCTACTTATCTTACTTCCATTGTGATGACCCACCCTCACCATCGCCTGGGAAGAGAAAGTGTCCAGTAGGGCGGAGGCGGAGGCCCAGAGGCAGGATGAAAAACCACAGTCTGAGAGCTGGTAGAGAGTGCCCGAGAGGCCTGGAGGAGACTTCGGACCTTCTTTCACAACTGCAGAGCCTCCTGGGGCCACACCTTGACAAAGGTGACTTTGGTCAGCTCTCCGGTCCAGACCCCCCAGGTGAGGTGGGCGAAAGAGCACCTGATGGAGCCTCCCAGTCCTCTCATGAGCCTATGGAAGATGCTGCTCCCATTCTCTCCCCGTTAGCTTCCCCGGATCCTCAAGCCAAGCATCCTCAGGATCTGGCCTCCACCCCATCACCAGGCCCAATGACCACCTCAGTCTCCTCCCTAAGTGCCTCCCAGCCACCAGAACCTTCCCTTCCCCTAGAACACCCCTCACCCGAGCCACCTGCACTTTTCCCTCACCCACCACACACCCCTGATCCTCTGGCCTGCTCTCCGCCTCCTCCAAAAGGCTTCACTGCTCCTCCCCTGCGGGACTCCACACTGATAACTCCATCTCACTGTGACTCAGTGGCACTTCCACTGGGCACCGTCCCTCAAAGCTTGTCTCCACATGAGGATTTGGTGGCTTCTGTCCCAGCCATCTCAGGCCTTGGTGGCTCAAACAGTCATGTTTCTGCCTCCTCCCGGTGGCAGGAGACTGCCAGAACCTCGTGCGCCTTTAACTCATCAGTCCAGCAAGATCCTCTTTCCCGCCACCCACCAGAGACCTGTCAGATGGAAGCTGGTAGCCTGTTTTTGCTCAGCTCTGATGGCCAGAATGTCGTGGGGATACAAGTCACAGAAACAGCCAAGGTCAACATTTGGGAAGAAAAAGAAAATGTTGGATCATTTACAAATCAAATGACCCCAGAAAAGCACTTAAATTCTTTGGGGAATTTGGCTAAATCATTGGATGCTGAGCAGGACACCACAAACCCAAAACCCTTCTGGAACATGGGAGAGAACTCGAAACAGCTGCCCGGACCTCAGAAGTGCTCAGATCCTAGGCTCTTGCAGGAAAGTTTTTGGAAGAATTATAGCCAGCTTTTCTGGGGCCTCCCCTCTCTGCACAGCGAGTCCCTGGTGGCTAACGCCTGGGTAACTGACAGGTCTTATACTTTACAGTCTCCTCCTTTCTTGTTCAATGAAATGTCCAATGTCTGCCCAATTCAAAGGGAGACTACAATGTCCCCACTGCTTTTCCAGGCCCAGCCCCTGTCCCACCGCCAACCCTTTATTTCATCCACACCCCAATTCCTGCCCACACCTATGGCTCAGGCCGAGGCTCAGGCCCATCTTCAGTCTTCTTTCCCAGTCCTATCTCCTGCTTTTCCATCCCTGATTAAGAACACTGGAGTAGCTTGCCCTGCATCGCAGAATAAAGTGCAAGCTCTCTCCCTACCTGAAACTCAGCACCCTGAATGGCCTTTGTTGAGGAAACAACTAGAAGGTAGGTTGGCTTTACCCTCTAGGGTCCAAAAATCTCAGGACGTCTTTAGTGTCTCCACTCCTAACCTTCCCCAGGAAAGTTTGACATCCATTCTGCCTGAGAACTTTCCAGTCAGTCCTGAACTCCGGAGACAACTGGAGCAACACATAAAAAAGTGGATCATCCAACACTGGGGCAACCTGGGAAGGATCCAAGAGTCTCTGGATCTGATGCAGCTTCGGGACGAATCACCAGGGACAAGTCAGGCCAAGGGCAAACCCAGTCCCTGGCAGTCCTCCACGTCCACAGGTGAAAGCAGCAAGGAGGCACAGAAGGTGAAGTTCCAGCTAGAGAGGGACCTGTGCCCACATCTGGGGCAAATTCTGGGTGAGACCCCACAAAATCTATCCAGGGACATGAAAAGCTTCCCACGGAAGGTTCTGGGGGTGACTTCTGAGGAGTCGGAAAGGAACTTGAGGAAGCCCTTGAGGAGTGACTCGGGAAGTGATTTATTAAGATGCACAGAGAGGACTCATATAGAAAACATCCTGAAAGCCCACATGGGCAGGAACTTGGGCCAGACCAACGAGGGCTTGATCCCCGTGCGTGTGCGTCGATCCTGGCTTGCTGTCAACCAGGCTCTTCCCGTGTCCAACACCCATGTGAAAACCAGCAATCTAGCAGCCCCGAAAAGTGGGAAAGCCTGTGTGAACACAGCCCAGGTGCTTTCCTTCCTCGAGCCGTGTACTCAGCAGGGGTTGGGAGCCCATATTGTGAGGTTTTGGGCCAAACACAGGTGGGGTCTACCCCTCAGGGTCCTCAAGCCCATTCAGTGCTTTAAACTGGAAAAGGTTTCATCCTTGTCCCTTACGCAGCTTGCTGGTCCCTCCTCAGCCACCTGTGAATCTGGGGCTGGCTCAGAAGTTGAGGTGGACATGTTCCTTAGAAAGCCACCAATGGCAAGTCTGAGAAAGCAGGTGCTGACCAAAGCATCTGATCACATGCCAGAGAGTCTTCTGGCCTCCTCACCTGCATGGAAGCAGTTCCAGAGGGCACCGCGAGGAATCCCATCTTGGAATGATCATGGGCCCTTGAAGCCTCCTCCAGCTGGACAGGAGGGCAGGTGGCCATCTAAGCCCCTCACGTACAGCCTCACAGGCAGCACCCAGCAGAGCAGGAGCTTAGGAGCCCAATCTTCAAAGGCTGGAGAGACAAGGGAGGCAGTGCCACAATGCAGAGTCCCCTTGGAAACCTGTATGCTGGCAAACCTCCAAGCCACAAGTGAGGATGTGCATGGTTTCGAGGCTCCAGGGACCAGCAAAAGCTCTCTACACCCTAGAGTGTCTGTCTCCCAAGATCCAAGAAAGCTGTGTCTTATGGAGGAGGTTGTTAGTGAATTTGAGCCTGGAATGGCCACAAAGTCAGAGACCCAGCCTCAAGTTTGTGCCGCTGTTGTGCTCCTTCCAGATGGGCAAGCATCTGTTGTGCCCCACGCTTCAGAGAATTTGGTTTCTCAAGTGCCCCAGGGCCATCTCCAGAGCATGCCTACTGGGAACATGCGGGCTTCCCAGGAGCTACATGACCTCATGGCAGCCAGAAGGAGCAAACTGGTGCAAGAGGAGCCCAGAAACCCAAACTGTCAAGGCTCATGCAAGAGCCAAAGGCCAATGTTTCCCCCTATTCACAAGAGTGAGAAGTCTAGGAAGCCCAACTTAGAAAAACATGAAGAAAGGCTTGAAGGATTGAGGACTCCTCAACTTACCCCAGTCAGGAAAACAGAAGACACCCATCAGGATGAAGGCGTCCAGCTACTGCCATCAAAGAAACAGCCTCCTTCAGTAAGCCACTTTGGAGAAAACATCAAGCAATTTTTTCAGTGGATTTTTTCAAAGAAAAAAAGCAAGCCAGCACCAGTCACTGCTGAGAGCCAAAAAACAGTAAAAAACAGATCATGTGTGTACAGCAGCAGTGCTGAAGCTCAGGGTCTCATGACGGCAGTTGGACAAATGCTGGACAAGAAAATGTCACTTTGCCATGCGCACCATGCCTCGAAGGTAAATCAGCACAAACAGAAGTTTCAAGCCCCAGTCTGTGGGTTTCCCTGCAACCACAGGCACCTCTTCTACTCAGAACATGGCAGAATACTGAGCTATGCAGCCAGCAGTCAACAAGCCACTCTCAAGAGCCAGGGTTGTCCCAACAGAGACAGGCAAATCAGAAATCAACAGCCCTTGAAAAGTGTGCGGTGCAACAATGAGCAATGGGGCCTGCGACATCCCCAAATCTTGCACCCCAAGAAAGCTGTATCCCCAGTCAGTCCCCCTCAGCACTGGCCGAAGACATCCGGTGCCTCTAGCCACCATCACCACTGTCCAAGGCACTGTCTTCTTTGGGAAGGTATC
MENLPFPLKLLSASSLNAPSSTPWVLDIFLTLVFALGFFFLLLPYLSYFHCDDPPSPSPGKRKCPVGRRRRPRGRMKNHSLRAGRECPRGLEETSDLLSQLQSLLGPHLDKGDFGQLSGPDPPGEVGERAPDGASQSSHEPMEDAAPILSPLASPDPQAKHPQDLASTPSPGPMTTSVSSLSASQPPEPSLPLEHPSPEPPALFPHPPHTPDPLACSPPPPKGFTAPPLRDSTLITPSHCDSVALPLGTVPQSLSPHEDLVASVPAISGLGGSNSHVSASSRWQETARTSCAFNSSVQQDPLSRHPPETCQMEAGSLFLLSSDGQNVVGIQVTETAKVNIWEEKENVGSFTNQMTPEKHLNSLGNLAKSLDAEQDTTNPKPFWNMGENSKQLPGPQKCSDPRLLQESFWKNYSQLFWGLPSLHSESLVANAWVTDRSYTLQSPPFLFNEMSNVCPIQRETTMSPLLFQAQPLSHRQPFISSTPQFLPTPMAQAEAQAHLQSSFPVLSPAFPSLIKNTGVACPASQNKVQALSLPETQHPEWPLLRKQLEGRLALPSRVQKSQDVFSVSTPNLPQESLTSILPENFPVSPELRRQLEQHIKKWIIQHWGNLGRIQESLDLMQLRDESPGTSQAKGKPSPWQSSTSTGESSKEAQKVKFQLERDLCPHLGQILGETPQNLSRDMKSFPRKVLGVTSEESERNLRKPLRSDSGSDLLRCTERTHIENILKAHMGRNLGQTNEGLIPVRVRRSWLAVNQALPVSNTHVKTSNLAAPKSGKACVNTAQVLSFLEPCTQQGLGAHIVRFWAKHRWGLPLRVLKPIQCFKLEKVSSLSLTQLAGPSSATCESGAGSEVEVDMFLRKPPMASLRKQVLTKASDHMPESLLASSPAWKQFQRAPRGIPSWNDHGPLKPPPAGQEGRWPSKPLTYSLTGSTQQSRSLGAQSSKAGETREAVPQCRVPLETCMLANLQATSEDVHGFEAPGTSKSSLHPRVSVSQDPRKLCLMEEVVSEFEPGMATKSETQPQVCAAVVLLPDGQASVVPHASENLVSQVPQGHLQSMPTGNMRASQELHDLMAARRSKLVQEEPRNPNCQGSCKSQRPMFPPIHKSEKSRKPNLEKHEERLEGLRTPQLTPVRKTEDTHQDEGVQLLPSKKQPPSVSHFGENIKQFFQWIFSKKKSKPAPVTAESQKTVKNRSCVYSSSAEAQGLMTAVGQMLDKKMSLCHAHHASKVNQHKQKFQAPVCGFPCNHRHLFYSEHGRILSYAASSQQATLKSQGCPNRDRQIRNQQPLKSVRCNNEQWGLRHPQILHPKKAVSPVSPPQHWPKTSGASSHHHHCPRHCLLWEGI
維真生物的人源(yuan)cDNA克隆中,雖(sui)然我們(men)大約70%的(de)克隆與RefSeq或GeneBank所發表的基因列序完全一(yi)致,但也有些(xie)克隆含有天然的單核(he)苷酸(suan)多(duo)態(tai)性(xing)而(er)包含一至多(duo)個(ge)SNPs, 因此與(yu)RefSeq或GeneBank所發表的列序會有些不同。我(wo)們強烈建議您在購買(mai)之前仔細將我們克(ke)隆的序(xu)列與您所要求的序(xu)列進(jin)行(xing)對比,以(yi)決定所購買(mai)的克隆是否符合您的要求。如果我們(men)預制的克隆不符合您的要求,我們(men)一般在1-2周(zhou)內可(ke)以提供(gong)一個與(yu)您所要(yao)求的序(xu)列完全一致的克隆。我們的每一個克隆與(yu)RefSeq(2013)標準序列相比(bi)都含(han)有(you)一個完整的ORF(Opening Reading Frame)。因為RefSeq數據庫會不斷更(geng)新,更(geng)新后的標準序列(lie)將來(lai)有(you)可能與我們現有(you)的克隆序列(lie)產(chan)生差異。我們所有(you)的克隆產(chan)品都(dou)經(jing)過二代(NextGen)測序驗證,并且保(bao)證網(wang)站上克隆的序列(lie)與您收到的克隆序列(lie)完全一致。在(zai)未(wei)經維真生物書面給予授權前,人源ORF cDNA克隆(long)不可轉售或贈(zeng)予買方以(yi)外的第三方。同時, 在(zai)使用任何維(wei)真產品之前,買(mai)方有責任確認該基因的使用(yong)不會侵犯到(dao)第三方的知識產權(quan)。此產品專門為基礎研(yan)究所開發,不適用(yong)于任何治(zhi)療或(huo)診斷應用(yong)。