產品名稱:人源cDNA克隆(long)
產(chan)品規格:500μL甘油菌液
產品運輸條件:常(chang)溫
產(chan)品(pin)儲存條件和(he)期限(xian):-80℃長期(qi)保存(cun)
產品特點:
? 人源(yuan)基因C-端融合 Flag & 6×His 標簽(qian);
? 含有(you)puromycin真核抗性基因,可篩選穩(wen)定轉染細(xi)胞株;
? 含有卡那霉素原核抗性(xing)基因(yin),可篩選重(zhong)組克隆,推薦使用濃度為(wei)30μg/ml。
產品使用(yong):
將(jiang)甘油(you)菌在新鮮配制的固體(ti)培養基(相應抗性)中劃線,37℃培養(yang)(yang)箱培養(yang)(yang)過夜(ye),挑取3個單克(ke)隆,分別接種于相應抗性的液體培養(yang)(yang)基中(zhong),37℃搖床培養(yang)(yang)過夜(ye),然后進行質粒(li)的提取和驗證(zheng)。
溫馨提(ti)示:考慮到實驗的嚴謹性,建議您(nin)拿(na)到(dao)產(chan)品的第一(yi)時間(jian)進行(xing)測序確認(ren)。
CGCCATGGCCGCCGCGGAGACCCAGTCGCTACGGGAGCAGCCAGAGATGGAAGATGCTAATTCTGAAAAGAGTATAAATGAAGAAAATGGAGAAGTATCAGAAGACCAGTCTCAAAATAAGCACAgtcgtcacaaaaAAAAGAAGCATAAACACAGAAGTAAACATAAGAAACATAAACATTCCTCAGAAGAAGACAAGGATAAAAAACATAAACATAAGCATAAACATAAGAAACACAAAAGAAAAGAGGTTATTGATGCTTCTGATAAAGAGGGTATGTCTCCAGCAAAAAGAACTAAACTTGATGATTTAGCTTTGCTAGAAGACTTGGAAAAACAGAGAGCCTTGATTAAGGCCGAACTTGATAATGAGTTAATGGAAGGAAAGGTCCAGTCTGGTATGGGGCTCATTTTGCAAGGTTATGAGTCTGGCTCTGAAGAAGAGGGGGAAATTCATGAAAAGGCAAGAAATGGAAATAGGTCTAGTACTAGATCTTCAAGTACAAAGGGGAAACTTGAACTTGTGGACAATAAAATTACTACAAAGAAACGAAGTAAAAGCAGATCCAAAGAACGGACTAGACATAGGTCTGATAAAAAGAAAAGTAAGGGGGGTATTGAAATCGTTAAAGAGAAAACAACTAGGAGCAAGTCAAAGGAGAGGAAAAAATCTAAAAGCCCATCCAAAAGAAGTAAGTCTCAAGATCAAGCAAGGAAATCAAAATCCCCTACCCTTAGAAGGCGATCTCAAGAGAAAATTGGTAAGGCCAGATCTCCTACTGATGATAAGGTTAAAATTGAAGATaaaagtaaatcAAAAGATAGGAAAAAATCCCCAATTATAAATGAAAGTAGAAGTCGCGATCGAGGTAAAAAATCCAGATCCCCAGTTGATTTAAGAGGTAAATCCAAAGACAGAAGGTCACGGTCCAAAGAGAGAAAATCAAAACGGTCTGAAACTGATAAAGAAAAGAAGCCAATTAAATCTCCCTCTAAAGATGCTTCATCTGGGAAAGAAAATAGGTCACCCAGCAGAAGACCTGGTCGTAGTCCTAAAAGAAGAAGTTTGTCTCCAAAACCACGTGATAAATCAAGAAGAAGCAGGTCTCCACTTTTGAATGATAGAAGATCTAAGCAGAGCAAATCCCCCTCGCGGACACTGTCTCCTGGGAGAAGAGCCAAGAGCCGATCCTTAGAAAGAAAACGACGAGAACCAGAGAGGAGACGACTTTCTTCTCCAAGAACACGACCTCGAGATGATATCCTCAGTAGACGTGAAAGATCAAAAGATGCCAGCCCCATCAATAGATGGTCTCCAACCCGAAGAAGAAGTAGATCTCCCATTAGAAGGAGGTCTCGTTCCCCACTCAGACGTAGCAGGTCTCCAAGAAGAAGAAGCAGATCTACTCGGAGAAGGGACAGAGGTCGGAGGAGCAGATCACGCTTGCGAAGGCGGTCTCGATCACGCGGTGGTCgtagacgaaggaGCAGAAGCAAAGTAAAGGAAGATAAATTTAAAGGAAGTCTTTCTGAAGGAATGAAAGTTGAGCAGGAATCTTCGTCTGATGATAACCTTGAAGACTTTGATGTAGAGGAAGAAGATGAAGAAGCCCTAATAGAACAGAGAAGAATCCAAAGGCAGGCAATTGTTCAGAAATATAAATACCTTGCTGAAGATAGCAACATGTCTGTGCCATCTGAACCAagcagcccccagagcaGTACGAGAACACGATCACCATCTCCAGATGACATTCTGGAGCGAGTAGCTGCTGATGTTAAAGAGTATGAACGGGAAAATGTTGATACATTTGAGGCCTCAGTGAAAGCCAAGCATAATCTAATGACAGTTGAACAGAATAATGGTTCATCTCAGAAGAAGTTGTTGGCACCTGATATGTTTACAGAATCTGATGATATGTTTGCTGCGTATTTTGATAGTGCTCGTCTTCGGGCCGCTGGCATTGGAAAAGATTTCAAAGAGAATCCCAACCTCAGAGATAACTGGACCGATGCAGAAGGCTATTATCGTGTGAACATAGGTGAAGTCCTAGATAAACGTTACAATGTGTATGGCTACACTGGGCAAGGTGTATTCAGTAATGTTGTACGAGCCAGAGATAATGCAAGAGCCAACCAAGAAGTGGCTGTAAAGATCATCAGAAACAATGAGCTCATGCAAAAGACTGGTTTAAAAGAATTAGAGTTCTTGAAAAAACTTAATGATGCTGATCCTGATGACAAATTTCATTGTCTGAGACTCTTAAGGCACTTCTATCACAAGCAGCATCTTTGTCTGGTATTCGAGCCTCTCAGCATGAACTTACGAGAGGTGTTAAAAAAATATGGTAAAGATGTTGGTCTTCATATTAAAGCTGTAAGATCCTATAGTCAGCAGTTGTTCCTGGCATTGAAACTCCTTAAAAGATGCAATATCCTACATGCAGATATCAAGCCAGACAATATCCTGGTTAATGAATCCAAAACTATTTTAAAGCTTTGCGATTTTGGGTCGGCTTCACATGTTGCGGATAATGACATAACACCTTATCTTTTCAGTAGATTTTATCGTGCTCCTGAAATCATTATAGGTAAAAGCTATGACTATGGTATAGATATGTGGTCTGTAGGTTGCACCTTATACGAACTCTATACTGGAAAAATTTTATTCCCTGGCAAAACCAATAACCATATGCTGAAGCTTGCAATGGATCTCAAAGGAAAGATGCCAAATAAGATGATTCGAAAAGGTGTGTTCAAAGATCAGCATTTTGATCAAAATCTCAACTTCATGTACATAGAAGTTGATAAAGTAACAGAGAGGGAGAAAGTTACTGTTATGAGCACCATTAATCCAACTAAGGACCTGTTGGCTGACTTGATTGGGTGCCAGAGACTTCCTGAAGACCAACGTAAGAAAGTACACCAGCTAAAGGACTTGTTGGACCAGATTCTGATGTTGGACCCAGCTAAACGAATTAGCATCAACCAGGCCCTACAGCACGCCTTCATCCAGGaaaaaatta
MAAAETQSLREQPEMEDANSEKSINEENGEVSEDQSQNKHSRHKKKKHKHRSKHKKHKHSSEEDKDKKHKHKHKHKKHKRKEVIDASDKEGMSPAKRTKLDDLALLEDLEKQRALIKAELDNELMEGKVQSGMGLILQGYESGSEEEGEIHEKARNGNRSSTRSSSTKGKLELVDNKITTKKRSKSRSKERTRHRSDKKKSKGGIEIVKEKTTRSKSKERKKSKSPSKRSKSQDQARKSKSPTLRRRSQEKIGKARSPTDDKVKIEDKSKSKDRKKSPIINESRSRDRGKKSRSPVDLRGKSKDRRSRSKERKSKRSETDKEKKPIKSPSKDASSGKENRSPSRRPGRSPKRRSLSPKPRDKSRRSRSPLLNDRRSKQSKSPSRTLSPGRRAKSRSLERKRREPERRRLSSPRTRPRDDILSRRERSKDASPINRWSPTRRRSRSPIRRRSRSPLRRSRSPRRRSRSTRRRDRGRRSRSRLRRRSRSRGGRRRRSRSKVKEDKFKGSLSEGMKVEQESSSDDNLEDFDVEEEDEEALIEQRRIQRQAIVQKYKYLAEDSNMSVPSEPSSPQSSTRTRSPSPDDILERVAADVKEYERENVDTFEASVKAKHNLMTVEQNNGSSQKKLLAPDMFTESDDMFAAYFDSARLRAAGIGKDFKENPNLRDNWTDAEGYYRVNIGEVLDKRYNVYGYTGQGVFSNVVRARDNARANQEVAVKIIRNNELMQKTGLKELEFLKKLNDADPDDKFHCLRLLRHFYHKQHLCLVFEPLSMNLREVLKKYGKDVGLHIKAVRSYSQQLFLALKLLKRCNILHADIKPDNILVNESKTILKLCDFGSASHVADNDITPYLFSRFYRAPEIIIGKSYDYGIDMWSVGCTLYELYTGKILFPGKTNNHMLKLAMDLKGKMPNKMIRKGVFKDQHFDQNLNFMYIEVDKVTEREKVTVMSTINPTKDLLADLIGCQRLPEDQRKKVHQLKDLLDQILMLDPAKRISINQALQHAFIQEKI
維真生物的人源(yuan)cDNA克隆中,雖然我們大約(yue)70%的克隆與RefSeq或GeneBank所發(fa)表(biao)的基因列序完全一致,但也有些克(ke)隆含(han)有天然的單核苷酸多態(tai)性而包含(han)一至多個SNPs, 因此與RefSeq或(huo)GeneBank所發(fa)表(biao)的列(lie)序會有些不同。我們強烈建議您在購(gou)買之前仔細將我們克(ke)隆的(de)序(xu)列(lie)與您所要求的(de)序(xu)列(lie)進(jin)行對比,以決定(ding)所購(gou)買的(de)(de)克(ke)隆(long)是(shi)否符(fu)合(he)您的(de)(de)要求。如果(guo)我(wo)們預制(zhi)的(de)(de)克(ke)隆(long)不(bu)符(fu)合(he)您的(de)(de)要求,我(wo)們一般在1-2周內可以提供一(yi)(yi)個與您所要求(qiu)的(de)(de)序(xu)列完全一(yi)(yi)致的(de)(de)克隆。我們的(de)(de)每一(yi)(yi)個克隆與RefSeq(2013)標準序列相比都含有一個(ge)完整(zheng)的ORF(Opening Reading Frame)。因(yin)為(wei)RefSeq數據庫(ku)會不斷(duan)更新(xin),更新(xin)后的(de)(de)標準序列(lie)將來有可能與(yu)我(wo)們(men)現有的(de)(de)克隆(long)序列(lie)產(chan)生差(cha)異。我(wo)們(men)所有的(de)(de)克隆(long)產(chan)品都(dou)經過二代(NextGen)測序驗證(zheng),并且(qie)保證(zheng)網站上克隆的(de)序列(lie)(lie)與(yu)您收到(dao)的(de)克隆序列(lie)(lie)完全一(yi)致(zhi)。在未經維真生物書面(mian)給予授(shou)權前,人源ORF cDNA克隆(long)不可轉售或(huo)贈予(yu)買方以外的第三(san)方。同時, 在(zai)使用任何維真產品之前,買方有責任確(que)認該基因的使用不會侵犯到第(di)三方的知識產(chan)權(quan)。此產(chan)品專門為基礎研究(jiu)所開發,不適用于任何治療(liao)或(huo)診斷(duan)應用。