產(chan)品名稱:人源cDNA克隆(long)
產品規(gui)格:500μL甘(gan)油(you)菌液
產品運輸條(tiao)件(jian):常溫
產品儲(chu)存條件(jian)和期(qi)限(xian):-80℃長期保存(cun)
產(chan)品特(te)點:
? 人源基(ji)因C-端融合(he) Flag & 6×His 標簽;
? 含(han)有puromycin真核抗(kang)性基因,可篩(shai)選穩定(ding)轉染細胞株;
? 含有卡那霉(mei)素原核抗性(xing)基因,可篩選重組(zu)克(ke)隆,推薦(jian)使用濃(nong)度為(wei)30μg/ml。
產品使用:
將甘油菌在新鮮配制的(de)固體培養(yang)基(相應抗性)中劃線,37℃培養(yang)箱培養(yang)過(guo)夜,挑取3個單克隆,分別接種于相應抗性的(de)液(ye)體培養(yang)基(ji)中,37℃搖床(chuang)培養(yang)過(guo)夜,然后進行質粒的(de)提取和驗證。
溫(wen)馨(xin)提示:考慮到實驗的嚴謹性,建議您拿到(dao)產品的第一時間進行測序確認(ren)。
ATGTCCAATGTTACCTTGAGAAAAATGTCTCCCACAGGAAATGAGATGAAGAGCACCACTCAGGGAACCACACGGAAGCAGCAGGATTTTCACGAGGTGAACAAAAGAAGAACTTTCTTACAGGATAACAGTTGGATAAAGAAACGCCCTGAAGAAGAAAAAGATGAAAATTACGGTAGGGTGGTGCTCAACCGACATAATTCCCATGATGCATTGGACAGGAAAGTAAATGAGAGAGATGTGCCAAAAGCTACAATTAGTCGGTACAGTTCTGATGACACTTTGGACAGGATCTCAGACAGAAATGATGCTGCTAAAACATATAAGGCCAATACCTTGGATAACCAACTAACCAATAGGAGCATGTCCATGTTTAGATCACTGGAAGTAACAAAGTTGCAACCTGGCGGTTCATTGAATGCCAACACCTCCAACACCATAGCATCCACTTCTGCTACTACTCCTGTAAAGAAGAAGAGGCAGTCCTGGTTTCCACCGCCCCCTCCAGGTTACAATGCCTCCTCGAGCACAGGAACCAGGAGACGGGAACCAGGTGTTCACCCTCCAATACCTCCAAAGCCCAGTTCTCCTGTTTCTTCTCCTAACCAGCTGAGACAGGATAATAGGCAGATACATCCACCTAAACCAGGTGTATATACAGAAACCAACAGATCTGCTGAAAGAAATATAAGTGAAGAATTGGATAATCTCATCAAAATGAACAAAAGCTTGAATAGGAATCAAGGTCTTGATAGTCTCTTCAGAGCAAATCCAAAGGTAGAAGAAAGAGAGAAAAGAGCCAAAAGCCTTGAAAGTCTCATCTATATGAGTACCCGGACAGATAAAGATGGCAAAGGAATCCAAAGCCTTGGAAGTCCGATTAAAGTTAATCAAAGGACTGACAAAAATGAGAAAGGAAGACAAAATCTCGAATCTGTTGCTAAAGTGAATGCCAGGATGAATAAAACGAGCAGAAGAAGTGAAGACCTTGATAATGCTACTGAAGTAAATCCCAAAGGACATGAAAATACCACTGGAAAAAAAGACCTTGATGGGCTTATTAAAGTGGATCCTGAAACAAATAAAAATATTACGAGGGGCCAGAGCCTTGATAATCTCATCAAAGTGACCCCTGAAGTAAAGAGAAGTAACCAAGGTTCCAAAGACCTTAATAACTTCATCAAAGTGTATCCAGGAACAGAAAAAAGTACTGAAGGGGGCCAAAGTCTCGACAGCCTCATTAAAGTGACTCCTGAAAGAAACAGAACTAACCAAGGGAACCAAGACTTGGAAAATCTTATCAAAGTGATCCCTTCAGCAAACAAAAGCAGTGAACAAGGTCTTGATGAACATATTAATGTCAGCCCCAAAGCTGTCAAAAACACTGATGGAAAACAAGATCTTGATAAACTCATCAAGGTGAATCCTGAAATTTTCACAAACAACCAAAGAAACCAAGATCTTGCTAACCTCATCAAAGTAAATCCTGCAGTAATCAGAAACAATCAGAGCCAAGACTTGGACAATCTTATTAAAGTGAAACCTTCAGCTCTTAGAAACACTAATCGAGACCAGAACCTGGAAAATTTAATTGAAGTAAATTCTCATGTGTCTGAAAACAAGAATGGAAGCTCTAACACTGGAGCCAAGCAGGCAGGACCACAGGATACTGTTGTGTACACAAGGACATATGTGGAGAATAGTAAATCACCCAAGGATGGATATCAGGAGAATATCTCTGGAAAATACATACAAACTGTTTATTCAACTTCTGATAGGTCTGTCATTGAAAGAGATATGTGCACTTACTGCCGAAAACCCTTGGGTGTAGAAACTAAAATGATTTTAGATGAATTACAAATTTGCTGCCATTCTACTTGCTTTAAGTGTGAAATATGCAAGCAGCCTTTGGAAAATCTACAAGCGGGTGATAGTATTTGGATTTATAGACAGACAATACACTGTGAACCTTGCTACTCTAAAATTATGGCAAAGTGGATTCCA
MSNVTLRKMSPTGNEMKSTTQGTTRKQQDFHEVNKRRTFLQDNSWIKKRPEEEKDENYGRVVLNRHNSHDALDRKVNERDVPKATISRYSSDDTLDRISDRNDAAKTYKANTLDNQLTNRSMSMFRSLEVTKLQPGGSLNANTSNTIASTSATTPVKKKRQSWFPPPPPGYNASSSTGTRRREPGVHPPIPPKPSSPVSSPNQLRQDNRQIHPPKPGVYTETNRSAERNISEELDNLIKMNKSLNRNQGLDSLFRANPKVEEREKRAKSLESLIYMSTRTDKDGKGIQSLGSPIKVNQRTDKNEKGRQNLESVAKVNARMNKTSRRSEDLDNATEVNPKGHENTTGKKDLDGLIKVDPETNKNITRGQSLDNLIKVTPEVKRSNQGSKDLNNFIKVYPGTEKSTEGGQSLDSLIKVTPERNRTNQGNQDLENLIKVIPSANKSSEQGLDEHINVSPKAVKNTDGKQDLDKLIKVNPEIFTNNQRNQDLANLIKVNPAVIRNNQSQDLDNLIKVKPSALRNTNRDQNLENLIEVNSHVSENKNGSSNTGAKQAGPQDTVVYTRTYVENSKSPKDGYQENISGKYIQTVYSTSDRSVIERDMCTYCRKPLGVETKMILDELQICCHSTCFKCEICKQPLENLQAGDSIWIYRQTIHCEPCYSKIMAKWIP
維真生物的(de)人(ren)源cDNA克(ke)隆中,雖然我們大(da)約70%的克隆與RefSeq或(huo)GeneBank所(suo)發表的基(ji)因列序完(wan)全(quan)一致,但也有些克隆含有天然的單核苷酸多態性而包含一至(zhi)多個(ge)SNPs, 因(yin)此與RefSeq或GeneBank所發表的列序會(hui)有些不同(tong)。我們(men)強烈建議您在購買之前仔細將我們克隆的序列與(yu)您所要(yao)求(qiu)的序列進行對比,以決(jue)定所購買的克隆是否符(fu)合(he)您的要求。如果我(wo)們預(yu)制的克隆不(bu)符(fu)合(he)您的要求,我(wo)們一般(ban)在1-2周內可以提(ti)供(gong)一個(ge)與您所(suo)要求的(de)序列完全一致(zhi)的(de)克(ke)隆。我們的(de)每一個(ge)克(ke)隆與RefSeq(2013)標準序列相比都含有一(yi)個完整的ORF(Opening Reading Frame)。因為RefSeq數據庫會不斷更新(xin),更新(xin)后(hou)的標準序列將(jiang)來(lai)有(you)可能(neng)與我們現有(you)的克(ke)隆序列產(chan)生差異。我們所有(you)的克(ke)隆產(chan)品都經(jing)過二代(NextGen)測序(xu)驗(yan)證,并且保證網站上克(ke)隆(long)的序(xu)列(lie)與您收到的克(ke)隆(long)序(xu)列(lie)完全(quan)一致。在(zai)未經維(wei)真生物書面給(gei)予授(shou)權前,人源ORF cDNA克隆不(bu)可(ke)轉(zhuan)售(shou)或贈予(yu)買方以外的第三方。同(tong)時, 在使用任何維真產品之前,買(mai)方有責任(ren)(ren)確認(ren)該基因(yin)的使用(yong)(yong)不會侵犯(fan)到(dao)第三方的知識(shi)產(chan)(chan)權。此產(chan)(chan)品專門(men)為基礎研(yan)究(jiu)所開發,不適(shi)用(yong)(yong)于任(ren)(ren)何治(zhi)療或診斷(duan)應用(yong)(yong)。