產品名稱(cheng):人源(yuan)cDNA克隆
產品(pin)規格:500μL甘(gan)油菌液
產(chan)品運輸(shu)條(tiao)件:常溫(wen)
產品儲存條件和期限:-80℃長期保存
產品特點(dian):
? 人源基因C-端融(rong)合(he) Flag & 6×His 標簽(qian);
? 含(han)有(you)puromycin真核抗性(xing)基因,可篩選(xuan)穩(wen)定轉染(ran)細胞株(zhu);
? 含有卡那霉素(su)原核(he)抗性基因,可篩選重組克隆,推(tui)薦(jian)使(shi)用濃度(du)為30μg/ml。
產品(pin)使用(yong):
將甘油菌(jun)在新鮮配制的固(gu)體(ti)培養基(相應抗性)中劃(hua)線(xian),37℃培(pei)養箱培(pei)養過夜,挑取(qu)3個單(dan)克隆,分別(bie)接種(zhong)于相應抗性(xing)的(de)液體(ti)培(pei)養基中,37℃搖床培(pei)養過夜,然(ran)后進(jin)行質(zhi)粒的(de)提(ti)取(qu)和驗證。
溫馨提示:考慮到實驗的(de)嚴(yan)謹性(xing),建議您拿到產品的第一(yi)時間進行測序確(que)認(ren)。
CGCCATGATAGCTGTCTCTTTTAAATGCCGTTGTCAAATTCTGAGGCGACTTACTAAAGATGAGAGTCCCTACACTAAATCCGCCAGCCAGACAAAGCCGCCTGATGGAGCGTTGGCTGTGAGGAGACAGAGCATCCCAGAGGAATTCAAGGGCTCCACAGTCGTCGAGCTGATGAAGAAGGAAGGCGCTACCCTGGGTCTGACGGTATCGGGAGGAATTGATAAGGATGGCAAGCCAGGAGTATCTAATCTGCGGCAAGGAGGAATTGCTGCTAGAAGTGACCAGCTGGATGTGGGTGACTACATCAAAGCAGTGAATGGAATCAACCTGGCCAAATTCCGCCATGACGAGATCATCAGCTTGCTGAAGAATGTGGGAGAAAGAGTGGTTCTTGAAGTAGAGTACGAGCTTCCACCGGTCTCTGTGCAAGGATCAAGTGTTATTTTCCGAACAGTGGAGGTCACATTACATAAAGAAGGCAATACCTTTGGTTTTGTAATTCGAGGGGGAGCACATGATGATAGAAATAAATCTCGTCCAGTTGTGATAACATGTGTTCGTCCTGGAGGGCCTGCTGACAGAGAGGGCACGATCAAACCCGGTGACAGGTTGCTCAGTGTGGATGGAATTCGGCTTCTTGGAACTACGCATGCTGAAGCCATGAGTATTCTTAAACAATGTGGACAAGAAGCAGCACTGCTGATAGAATATGATGTCTCAGTAATGGACTCTGTGGCAACAGCATCCGGGCCACTACTAGTCGAAGTTGCCAAAACTCCTGGTGCCAGCCTTGGGGTTGCCCTAACTACCTCGATGTGCTGTAACAAACAAGTCATTGTCATAGACAAAATCAAATCTGCAAGTATTGCAGACAGATGTGGCGCATTGCATGTGGGAGATCACATCCTCTCCATCGATGGAACCAGCATGGAGTACTGTACACTTGCAGAAGCAACCCAGTTCCTGGCCAACACCACTGACCAGGTCAAGCTTGAGATCCTTCCCCATCATCAGACCCGGCTGGCCCTAAAGGGGCCCGACCATGCAGCTTTGGTGTCTTCATCCTTCTCTCCTACCTCCATGAGTGCATACAGCCTGAGTTCCCTGAACATGGGGACTCTACCTCGAAGCCTCTACTCCACCAGCCCACGTGGAACCATGATGAGGAGGAGACTGAAAAAGAAAGACTTCAAAAGCTCATTGTCCTTAGCCTCCAGCACAGTAGGATTGGCTGGGCAGGTTGTTCACACAGAAACCACAGAGGTTGTGCTGACGGCAGATCCTGTCACAGGATTTGGGATCCAACTGCAGGGCAGTGTGTTTGCCACAGAAACTCTCTCTTCTCCACCTCTGATTTCCTATATCGAAGCTGACAGCCCAGCAGAGAGATGTGGGGTGCTACAGATTGGAGACAGAGTGATGGCCATCAATGGAATTCCAACAGAAGACAGCACCTTCGAAGAAGCCAGTCAGCTCCTCCGAGACTCTTCAATCACGAGCAAGGTCACACTGGAAATCGAGTTTGATGTTGCAGAGTCTGTCATCCCAAGTAGTGGAACATTTCATGTAAAGCTGCCTAAGAAGCACAATGTGGAACTTGGAATAACCATAAGTTCACCATCCAGTAGAAAACCAGGAGACCCCCTCGTCATTTCAGATATCAAGAAAGGGAGTGTGGCACACAGAACTGGGACCCTGGAACTTGGGGATAAATTGCTCGCAATAGATAATATCCGGCTGGACAACTGTTCCATGGAAGATGCAGTTCAGATCCTCCAGCAATGTGAAGACCTGGTGAAGCTCAAAATCCGCAAAGATGAAGATAATTCAGATGAGCAAGAAAGTTCCGGAGCAATTATTTACACCGTGGAGCTTAAACGCTACGGGGGGCCCCTTGGCATCACAATTTCAGGAACTGAAGAGCCGTTTGATCCTATAATCATTTCAAGCCTCACTAAAGGGGGATTAGCTGAAAGAACTGGCGCAATCCACATAGGAGACCGAATCCTAGCCATCAATAGCAGCAGCTTGAAAGGGAAGCCTCTGAGTGAAGCCATCCATTTGTTACAGATGGCAGGAGAGACTGTCACCTTGAAAATTAAGAAACAGACAGATGCCCAGTCAGCATCGAGCCCCAAGAAGTTCCCTATTTCTAGCCATTTGAGTGACCTGGGGGATGTGGAGGAGGACTCCTCACCAGCACAGAAGCCAGGCAAGCTCTCCGACATGTACCCCTCCACGGTGCCCAGTGTGGACAGTGCTGTGGATTCATGGGATGGGTCTGCAATAGACACCAGCTATGGAACTGAAGGCACTAGTTTTCAGGCCTCAGGATACAATTTCAACACCTATGACTGGAGGAGTCCAAAACAGAGAGGCAGCTTGTCCCCAGTCACTAAGCCTCGAAGCCAGACTTACCCAGATGTGGGGCTGAGTTATGAAGACTGGGACCGGTCCACAGCCAGTGGTTTTGCAGGGGCTGCCGATAGTGCAGAGACAGAACAAGAGGAGAACTTCTGGTCTCAAGCGCTGGAGGATTTGGAAACCTGCGGACAGTCAGGAATTCTGAGAGAACTGGAGGAGAAAGCTGACAGGCGTGTGTCTTTGAGAAACATGACTCTCTTGGCAACAATCATGTCGGGGAGCACGATGAGTTTGAATCATGAGGCTCCAACACCTCGCAGTCAGCTGGGGCGACAGGCCAGCTTCCAGGAGCGCAGCAGCTCGCGGCCGCACTACAGCCAAACAACTCGGAGCAACACCCTGCCTTCAGATGTGGGTAGGAAGTCAGTAACCCTGAGAAAAATGAAACAAGAAATAAAGGAGATCATGTCTCCAACTCCTGTGGAGCTGCACAAGGTGACCTTGTACAAGGACTCTGACATGGAGGACTTTGGGTTCAGTGTAGCAGATGGCTTACTGGAGAAAGGAGTGTATGTCAAAAATATTCGCCCAGCTGGGCCAGGAGATCTTGGTGGCTTAAAGCCCTATGACAGGCTCTTACAGGTGAATCATGTCCGAACCAGAGACTTTGACTGCTGCCTTGTTGTGCCCCTCATAGCAGAATCCGGGAATAAGCTGGACCTGGTTATTAGTAGAAACCCACTGGCTTCACAGAAGTCTATAGACCAACAGAGTCTACCAGGAGATTGGAGTGAACAGAACAGTGCTTTTTTCCAGCAGCCTAGCCACGGTGGTAATTTGGAGACACGAGAACCCACTAATACATTAA
MIAVSFKCRCQILRRLTKDESPYTKSASQTKPPDGALAVRRQSIPEEFKGSTVVELMKKEGATLGLTVSGGIDKDGKPGVSNLRQGGIAARSDQLDVGDYIKAVNGINLAKFRHDEIISLLKNVGERVVLEVEYELPPVSVQGSSVIFRTVEVTLHKEGNTFGFVIRGGAHDDRNKSRPVVITCVRPGGPADREGTIKPGDRLLSVDGIRLLGTTHAEAMSILKQCGQEAALLIEYDVSVMDSVATASGPLLVEVAKTPGASLGVALTTSMCCNKQVIVIDKIKSASIADRCGALHVGDHILSIDGTSMEYCTLAEATQFLANTTDQVKLEILPHHQTRLALKGPDHAALVSSSFSPTSMSAYSLSSLNMGTLPRSLYSTSPRGTMMRRRLKKKDFKSSLSLASSTVGLAGQVVHTETTEVVLTADPVTGFGIQLQGSVFATETLSSPPLISYIEADSPAERCGVLQIGDRVMAINGIPTEDSTFEEASQLLRDSSITSKVTLEIEFDVAESVIPSSGTFHVKLPKKHNVELGITISSPSSRKPGDPLVISDIKKGSVAHRTGTLELGDKLLAIDNIRLDNCSMEDAVQILQQCEDLVKLKIRKDEDNSDEQESSGAIIYTVELKRYGGPLGITISGTEEPFDPIIISSLTKGGLAERTGAIHIGDRILAINSSSLKGKPLSEAIHLLQMAGETVTLKIKKQTDAQSASSPKKFPISSHLSDLGDVEEDSSPAQKPGKLSDMYPSTVPSVDSAVDSWDGSAIDTSYGTEGTSFQASGYNFNTYDWRSPKQRGSLSPVTKPRSQTYPDVGLSYEDWDRSTASGFAGAADSAETEQEENFWSQALEDLETCGQSGILRELEEKADRRVSLRNMTLLATIMSGSTMSLNHEAPTPRSQLGRQASFQERSSSRPHYSQTTRSNTLPSDVGRKSVTLRKMKQEIKEIMSPTPVELHKVTLYKDSDMEDFGFSVADGLLEKGVYVKNIRPAGPGDLGGLKPYDRLLQVNHVRTRDFDCCLVVPLIAESGNKLDLVISRNPLASQKSIDQQSLPGDWSEQNSAFFQQPSHGGNLETREPTNTL
維(wei)真(zhen)生物的人(ren)源(yuan)cDNA克隆中,雖然我們大約70%的(de)克隆與RefSeq或GeneBank所發表的(de)基因列序完全一致,但(dan)也有(you)些克隆含(han)有(you)天然的(de)單核苷酸多(duo)態(tai)性而包含(han)一至多(duo)個SNPs, 因此與RefSeq或GeneBank所發表的列(lie)序會有(you)些不同(tong)。我們強烈建(jian)議您在購買之前仔(zi)細將我們克隆的(de)序列與您所(suo)要求的(de)序列進行對比,以決定(ding)所購買(mai)的(de)克(ke)隆是否符合(he)您的(de)要求(qiu)。如(ru)果我們(men)預制(zhi)的(de)克(ke)隆不符合(he)您的(de)要求(qiu),我們(men)一(yi)般在1-2周內可以提供一(yi)個與(yu)(yu)您所要求的序(xu)列完全一(yi)致(zhi)的克(ke)隆(long)。我們的每一(yi)個克(ke)隆(long)與(yu)(yu)RefSeq(2013)標(biao)準序列相比都含有一個完整(zheng)的(de)ORF(Opening Reading Frame)。因為RefSeq數據(ju)庫會不斷(duan)更新(xin),更新(xin)后的標準序(xu)列將來有(you)可能與(yu)我們現(xian)有(you)的克(ke)隆(long)序(xu)列產生差異(yi)。我們所有(you)的克(ke)隆(long)產品都經過(guo)二代(NextGen)測(ce)序驗證,并且保證網站(zhan)上(shang)克(ke)隆的序列與您收到的克(ke)隆序列完全一(yi)致。在未(wei)經維真生物書面(mian)給(gei)予授權前,人源ORF cDNA克隆(long)不可轉售或贈(zeng)予買方以(yi)外的第三方。同時(shi), 在使(shi)用任何維真產(chan)品之前,買方(fang)有責任確認該基因(yin)的使(shi)用(yong)不(bu)會(hui)侵犯到第三(san)方(fang)的知識(shi)產(chan)權。此產(chan)品(pin)專門為基礎研究(jiu)所(suo)開發,不(bu)適用(yong)于任何(he)治療或(huo)診斷(duan)應用(yong)。