產(chan)品名稱:人源cDNA克隆
產品規格:500μL甘油(you)菌液(ye)
產品運輸條(tiao)件:常溫
產(chan)品儲存條(tiao)件和期限:-80℃長期保(bao)存(cun)
產品(pin)特點:
? 人源基(ji)因C-端融合 Flag & 6×His 標簽;
? 含有(you)puromycin真核抗(kang)性基因,可篩選穩定轉(zhuan)染細胞株;
? 含有卡那霉素原核(he)抗性(xing)基因,可篩選重組克(ke)隆,推薦使用濃度為30μg/ml。
產品使用:
將甘(gan)油菌在新鮮配制的固體培(pei)養(yang)基(相應抗性)中劃線(xian),37℃培養(yang)(yang)箱培養(yang)(yang)過夜,挑取3個單(dan)克隆,分(fen)別接種于相應抗性的液體培養(yang)(yang)基中,37℃搖床(chuang)培養(yang)(yang)過夜,然后進行質(zhi)粒的提取和驗證(zheng)。
溫馨提示:考(kao)慮到實(shi)驗(yan)的嚴(yan)謹性,建(jian)議您拿到(dao)產品(pin)的第一時間進行測序(xu)確認。
ATGGCGGAGCGTGGCGGGGACGGGGGCGAGAGTGAACGATTCAACCCGGGGGAGCTCAGGATGGCCCAACAGCAGGCCTTGAGGTTCCGAGGTCCGGCTCCCCCACCAAATGCAGTGATGCGAGGCCCACCACCTCTGATGCGACCTCCTCCACCTTTTGGTATGATGCGAGGCCCTCCTCCACCACCACGGCCGCCCTTTGGACGTCCTCCTTTTGATCCTAATATGCCGCCAATGCCTCCTCCAGGAGGGATACCTCCACCTATGGGCCCTCCACACCTCCAGAGACCACCTTTCATGCCTCCTCCCATGAGTTCCATGCCTCCTCCTCCGGGTATGATGTTTCCACCAGGAATGCCTCCTGTGACTGCTCCTGGTACTCCAGCACTACCTCCTACGGAGGAGATATGGGTTGAAAATAAAACTCCAGATGGGAAGGTTTATTATTATAATGCTCGGACACGTGAATCTGCATGGACCAAGCCAGATGGAGTTAAGGTTATTCAGCAATCAGAACTGACACCTATGCTTGCAGCCCAGGCACAGGTTCAGGCTCAGGCCCAGGCGCAGGCTCAGGCCCAGGCGCAGGCTCAGGCCCAGGCACAAGCTCAGGCCCAGGCTCAGGCTCAGGCCCAGGCCCAGGCCCAGGCCCAGGCCCAGGCCCAAGCCCAAGCCCAGGCCCAGGCTCAGGCTCAGGCACAAGCTCAGGCCCAGGCCCAGGCTCAGGTCCAGGCCCAGGTCCAGGCACAAGTGCAAGCACAAGCAGTTGGAGCTTCCACCCCTACGACCAGTAGCCCAGCACCTGCAGTATCCACTTCAACATCATCATCCACCCCTTCCTCTACCACTTCTACCACAACAACTGCTACTTCAGTTGCGCAGACAGTATCAACACCCACAACACAAGATCAGACCCCAAGTTCTGCTGTTTCAGTTGCCACGCCTACAGTTAGTGTTTCAACTCCTGCTCCTACAGCCACACCTGTGCAAACCGTTCCCCAGCCGCACCCTCAGACGTTACCTCCTGCTGTTCCTCATTCAGTACCTCAGCCAACAACAGCAATACCTGCTTTTCCACCAGTAATGGTACCTCCGTTTCGTGTTCCCCTTCCTGGCATGCCAATTCCACTTCCAGGTGTAGCAATGATGCAAATAGTCAGCTGCCCGTATGTAAAGACAGTCGCTACCACCAAGACCGGTGTATTGCCAGGAATGGCCCCTCCTATCGTACCCATGATACATCCCCAGGTTGCTATTGCAGCTTCACCTGCTACCTTAGCTGGAGCAACAGCAGTTTCTGAATGGACTGAATATAAAACAGCAGATGGGAAGACATATTATTATAATAATAGAACATTAGAATCAACCTGGGAAAAACCCCAAGAACTAAAGGAAAAAGAAAAGTTAGAAGAGAAGATTAAAGAGCCAATTAAAGAACCCTCTGAAGAGCCTCTGCCAATGGAGACGGAGGAGGAGGATCCTAAAGAAGAGCCTATAAAGGAGATAAAGGAGGAGCCCAAAGAAGAGGAGATGACTGAAGAAGAAAAGGCTGCCCAGAAGGCAAAGCCAGTTGCTACTGCTCCTATTCCTGGTACTCCATGGTGTGTCGTTTGGACTGGTGATGAGCGGGTCTTCTTTTATAATCCCACCACTCGTCTTTCTATGTGGGACCGACCTGATGATCTGATTGGCAGGGCAGATGTTGACAAAATTATTCAGGAGCCCCCTCATAAAAAAGGAATGGAGGAATTGAAGAAACTAAGGCACCCAACTCCGACAATGCTGTCGATCCAAAAGTGGCAATTCTCTATGAGTGCAATTAAAGAGGAACAAGAATTAATGGAAGAAATTAATGAAGATGAGCCTGTTAAAGCAAAAAAACGGAAGAGAGACGATAATAAAGACATTGACTCAGAGAAAGAAGCTGCCATGGAAGCTGAAATTAAAGCTGCCCGAGAAAGGGCCATTGTCCCTCTGGAGGCTCGAATGAAGCAGTTCAAGGACATGCTGCTAGAGAGAGGGGTGTCTGCTTTTTCAACGTGGGAGAAGGAGTTGCACAAGATAGTTTTTGATCCCCGGTACTTACTTCTCAATCCTAAAGAGAGAAAACAGGTGTTTGATCAGTATGTAAAGACCAGGGCAGAGGAAGAACGCAGGGAAAAGAAAAATAAAATAATGCAAGCCAAGGAAGATTTCAAAAAAATGATGGAAGAAGCAAAATTTAATCCAAGAGCAACTTTTAGTGAATTTGCAGCCAAGCATGCTAAAGATTCAAGATTCAAAGCAATTGAAAAGATGAAAGACCGAGAAGCCTTGTTTAATGAGTTTGTGGCCGCTGCTAGGAAGAAAGAGAAAGAAGATTCGAAGACCAGAGGTGAGAAGATTAAATCGGATTTCTTTGAACTATTATCTAATCATCACTTGGACAGTCAGTCTCGATGGAGCAAAGTAAAAGACAAAGTAGAAAGTGATCCACGTTACAAAGCAGTAGATAGTTCATCAATGAGAGAAGACCTTTTCAAACAGTACATTGAAAAAATAGCCAAGAATTTAGACTCAGAAAAAGAAAAGGAGCTTGAAAGGCAAGCCCGCATTGAGGCAAGCCTTCGAGAACGAGAAAGGGAGGTTCAAAAGGCCCGTTCAGAACAAACAAAAGAAATAGATCGAGAGAGAGAGCAGCACAAACGAGAAGAAGCTATCCAGAATTTCAAAGCTCTTCTGTCTGACATGGTACGTTCTTCAGATGTGTCATGGTCTGATACTCGTAGGACCCTCCGAAAAGATCACCGCTGGGAATCTGGATCCTTATTGGAAAGAGAGGAGAAAGAGAAGCTTTTTAATGAACACATTGAAGCACTTACCAAAAAAAAGAGAGAGCACTTTAGGCAACTTCTGGATGAAACTTCTGCAATTACCTTAACATCCACGTGGAAAGAAGTAAAAAAAATCATTAAGGAAGATCCTCGATGTATTAAGTTCTCCTCCAGTGACAGGAAAAAACAAAGAGAATTTGAAGAATATATCAGAGACAAATATATCACAGCCAAAGCTGACTTCAGGACGCTTTTGAAAGAGACCAAATTTATAACATATAGATCCAAAAAATTAATCCAAGAATCAGATCAGCACCTGAAAGATGTAGAAAAAATTTTACAGAATGACAAACGGTATCTAGTACTGGACTGTGTGCCAGAGGAGAGGCGTAAACTGATTGTGGCATATGTTGATGACCTGGATCGCCGGGGTCCACCCCCACCTCCCACAGCATCGGAGCCCACGAGACGATCAACAAAA
MAERGGDGGESERFNPGELRMAQQQALRFRGPAPPPNAVMRGPPPLMRPPPPFGMMRGPPPPPRPPFGRPPFDPNMPPMPPPGGIPPPMGPPHLQRPPFMPPPMSSMPPPPGMMFPPGMPPVTAPGTPALPPTEEIWVENKTPDGKVYYYNARTRESAWTKPDGVKVIQQSELTPMLAAQAQVQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQVQAQVQAQVQAQAVGASTPTTSSPAPAVSTSTSSSTPSSTTSTTTTATSVAQTVSTPTTQDQTPSSAVSVATPTVSVSTPAPTATPVQTVPQPHPQTLPPAVPHSVPQPTTAIPAFPPVMVPPFRVPLPGMPIPLPGVAMMQIVSCPYVKTVATTKTGVLPGMAPPIVPMIHPQVAIAASPATLAGATAVSEWTEYKTADGKTYYYNNRTLESTWEKPQELKEKEKLEEKIKEPIKEPSEEPLPMETEEEDPKEEPIKEIKEEPKEEEMTEEEKAAQKAKPVATAPIPGTPWCVVWTGDERVFFYNPTTRLSMWDRPDDLIGRADVDKIIQEPPHKKGMEELKKLRHPTPTMLSIQKWQFSMSAIKEEQELMEEINEDEPVKAKKRKRDDNKDIDSEKEAAMEAEIKAARERAIVPLEARMKQFKDMLLERGVSAFSTWEKELHKIVFDPRYLLLNPKERKQVFDQYVKTRAEEERREKKNKIMQAKEDFKKMMEEAKFNPRATFSEFAAKHAKDSRFKAIEKMKDREALFNEFVAAARKKEKEDSKTRGEKIKSDFFELLSNHHLDSQSRWSKVKDKVESDPRYKAVDSSSMREDLFKQYIEKIAKNLDSEKEKELERQARIEASLREREREVQKARSEQTKEIDREREQHKREEAIQNFKALLSDMVRSSDVSWSDTRRTLRKDHRWESGSLLEREEKEKLFNEHIEALTKKKREHFRQLLDETSAITLTSTWKEVKKIIKEDPRCIKFSSSDRKKQREFEEYIRDKYITAKADFRTLLKETKFITYRSKKLIQESDQHLKDVEKILQNDKRYLVLDCVPEERRKLIVAYVDDLDRRGPPPPPTASEPTRRSTK
維真(zhen)生物的人源cDNA克隆(long)中,雖然我(wo)們大約70%的克隆與RefSeq或GeneBank所發表的基(ji)因列序(xu)完全一致,但也有(you)些(xie)克隆含有(you)天然的單核苷酸(suan)多態(tai)性而包(bao)含一至(zhi)多個SNPs, 因此與RefSeq或(huo)GeneBank所發(fa)表的列(lie)序會有些不同(tong)。我們強烈(lie)建議您在購買(mai)之前(qian)仔細將(jiang)我們克(ke)隆(long)的序列與您所(suo)要求的序列進行對比,以決定所購買的(de)克隆是否符(fu)合(he)您的(de)要求。如(ru)果我(wo)(wo)們(men)預(yu)制(zhi)的(de)克隆不符(fu)合(he)您的(de)要求,我(wo)(wo)們(men)一(yi)般(ban)在1-2周(zhou)內可以提供一(yi)(yi)個(ge)(ge)與您所要(yao)求的(de)序列完全一(yi)(yi)致的(de)克隆。我們的(de)每一(yi)(yi)個(ge)(ge)克隆與RefSeq(2013)標準序(xu)列相(xiang)比都(dou)含有一個(ge)完整的ORF(Opening Reading Frame)。因為RefSeq數據庫會不斷更新(xin),更新(xin)后(hou)的標(biao)準序列(lie)將來有可(ke)能與我們現有的克隆序列(lie)產生(sheng)差異。我們所(suo)有的克隆產品都經(jing)過二代(NextGen)測序驗(yan)證,并且保證網站上克(ke)隆的序列(lie)與您收到的克(ke)隆序列(lie)完全一致。在未經(jing)維(wei)真(zhen)生物書面(mian)給予授權前,人(ren)源ORF cDNA克(ke)隆不可轉(zhuan)售或贈予買方(fang)以外(wai)的第三(san)方(fang)。同時, 在使用任(ren)何維(wei)真產品之前,買方有(you)責任確認該基因的使用不(bu)會侵犯到第(di)三方的知(zhi)識產(chan)權(quan)。此產(chan)品專門為基礎(chu)研究所開(kai)發,不(bu)適用于任何治(zhi)療或(huo)診斷應用。