產(chan)品名稱:人源(yuan)cDNA克隆
產品規格:500μL甘油菌液
產(chan)品運輸(shu)條(tiao)件:常溫
產品儲存條(tiao)件(jian)和期(qi)限:-80℃長(chang)期保存
產品特點:
? 人源基因C-端融合 Flag & 6×His 標簽;
? 含有puromycin真核抗性基(ji)因,可篩(shai)選(xuan)穩定轉(zhuan)染(ran)細胞株(zhu);
? 含有卡(ka)那霉素原(yuan)核抗(kang)性基因,可(ke)篩選(xuan)重組克隆,推薦(jian)使用濃度為30μg/ml。
產品(pin)使用:
將甘油菌在新(xin)鮮(xian)配制的固(gu)體培養(yang)基(相應抗性(xing))中劃線,37℃培(pei)養箱培(pei)養過(guo)(guo)夜,挑取3個單克隆,分別(bie)接(jie)種于相應抗性的液體培(pei)養基中,37℃搖床培(pei)養過(guo)(guo)夜,然后進行質粒的提(ti)取和驗證(zheng)。
溫馨提(ti)示:考慮到(dao)實(shi)驗的嚴謹性,建議您(nin)拿(na)到產品(pin)的(de)第一時間進行測序確(que)認。
ATGTCTGCCTACTACAGGAATAACTGGTCTGAGGAAGACCCAGATTACCCTGACTATTCAGGGTCTCAGAACCGTACGCAGGGGTATTTGAAAACTCAAGGTTATCCAGATGTTCCAGGTCCTCTGAACAATCCAGACTACCCCGGCACCAGGAGCAATCCATACTCTGTAGCCTCCAGAACACGTCCAGACTATCCTGGGTCTCTGGCAGAACCAAATTATCCTAGATCTCTGAGTAATCCAGACTATTCTGGCACCAGAAGCAATGCATACTCTGCAGCCTCTAGAACAAGCCCAGACCATCCTACCTCTCTACCAGAGCCAGATTATAGTGAATTTCAGAGTCATCCCTACCACCGAGCATCATCCAGACAACCAGACTACCCTGGATCTCAACGAAATCCTGATTTTGCAGGCTCCAGCAGCAGTGGAAACTATGCAGGCTCCAGAACACATCCAGATCATTTTGGCTCCTTAGAACCGGACTACCCTGGAGCTCAGAGCAACTCTGATCATCCTGGACCCAGAGCCAATTTGAACCATCCAGGATCCAGAAAGAATCTGGAACATACAAGTTTTAGAATCAATCCATACGCAGACTCTCTGGGAAAGCCTGATTATCCAGGCGCTGACATTCAACCTAACTCTCCACCCTTTTTTGGGGAGCCAGACTATCCCAGTGCTGAGGACAATCAGAACTTGCCAAGCACTTGGAGAGAACCTGATTATTCAGATGCTGAGAATGGTCATGATTATGGCTCTTCTGAGACCCCAAAGATGACCAGGGGGGTGCTCAGCAGAACATCTTCAATCCAGCCCTCATTTCGTCACAGGAGTGATGACCCCGTGGGCAGTCTTTGGGGAGAGAATGATTACCCTGAAGGCATTGAAATGGCATCCATGGAGATGGCAAACTCATATGGCCACTCTCTGCCAGGTGCTCCTGGAAGTGGCTATGTGAACCCTGCTTATGTAGGTGAAAGTGGTCCTGTCCATGCTTATGGAAACCCACCATTGTCTGAATGTGATTGGCACAAGTCACCCCAAGGACAGAAGTTAATCGCATCCCTTATACCCATGACATCCAGAGACAGAATTAAAGCCATCAGGAACCAGCCAAGGACCATGGAAGAGAAAAGGAACCTTAGGAAAATAGTTGACAAAGAAAAAAGCAAACAGACCCATCGTATCCTTCAGCTCAATTGCTGTATTCAGTGTCTGAACTCCATTTCCCGGGCTTATCGGAGATCCAAGAACAGCCTGTCGGAAATTCTGAATTCCATCAGCCTGTGGCAGAAGACGCTGAAGATCATTGGAGGCAAGTTTGGAACCAGCGTCCTCTCCTATTTCAACTTTCTGAGATGGCTTTTGAAGTTCAACATTTTCTCATTCATCCTGAACTTCAGCTTCATCATAATCCCTCAGTTTACCGTGGCCAAAAAGAACACCCTCCAGTTCACTGGGCTGGAGTTTTTCACTGGGGTGGGTTATTTTAGGGACACAGTGATGTACTATGGCTTTTACACCAATTCCACCATCCAGCACGGGAACAGCGGGGCATCCTACAACATGCAGCTGGCCTACATCTTCACAATCGGAGCATGCTTGACCACCTGCTTCTTCAGTTTGCTGTTCAGCATGGCCAAGTATTTCCGGAACAACTTCATTAATCCCCACATTTACTCCGGAGGGATCACCAAGCTGATCTTTTGCTGGGACTTCACTGTCACTCATGAAAAAGCTGTGAAGCTAAAACAGAAGAATCTTAGCACTGAGATAAGGGAGAACCTGTCAGAGCTCCGTCAGGAGAATTCCAAGTTGACGTTCAATCAGCTGCTGACCCGCTTCTCTGCCTACATGGTAGCCTGGGTTGTCTCTACAGGAGTGGCCATAGCCTGCTGTGCAGCCGTTTATTACCTGGCTGAGTACAACTTAGAGTTCCTGAAGACACACAGTAACCCTGGGGCGGTGCTGTTACTGCCTTTCGTTGTGTCCTGCATTAATCTGGCCGTGCCATGCATCTACTCCATGTTCAGGCTTGTGGAGAGGTACGAGATGCCACGGCACGAAGTCTACGTTCTCCTGATCCGAAACATCTTTTTGAAAATATCAATCATTGGCATTCTTTGTTACTATTGGCTCAACACCGTGGCCCTGTCTGGTGAAGAGTGTTGGGAAACCCTCATTGGCCAGGACATCTACCGGCTCCTTCTGATGGATTTTGTGTTCTCTTTAGTCAATTCCTTCCTGGGGGAGTTTCTGAGGAGAATCATTGGGATGCAACTGATCACAAGTCTTGGCCTTCAGGAGTTTGACATTGCCAGGAACGTTCTAGAACTGATCTATGCACAAACTCTGGTGTGGATTGGCATCTTCTTCTGCCCCCTGCTGCCCTTTATCCAAATGATTATGCTTTTCATCATGTTCTACTCCAAAAATATCAGCCTGATGATGAATTTCCAGCCTCCGAGCAAAGCCTGGCGGGCCTCACAGATGATGACTTTCTTCATCTTCTTGCTCTTTTTCCCATCCTTCACCGGGGTCTTGTGCACCCTGGCCATCACCATCTGGAGAATCATCACCTATCTTTACTGGCAGATCACAGAGGGAAGGAAGATTATGATAAGGCTGCTCCATGAGCAGATCATTAATGAGGGCAAAGATAAAATGTTCCTGATAGAAAAATTGATCAAGCTGCAGGATATGGAGAAGAAAGCAAACCCCAGCTCACTTGTTCTGGAAAGGAGAGAGGTGGAGCAACAAGGCTTTTTGCATTTGGGGGAACATGATGGCAGTCTTGACTTGCGATCTAGAAGATCAGTTCAAGAAGGTAATCCAAGGGCC
MSAYYRNNWSEEDPDYPDYSGSQNRTQGYLKTQGYPDVPGPLNNPDYPGTRSNPYSVASRTRPDYPGSLAEPNYPRSLSNPDYSGTRSNAYSAASRTSPDHPTSLPEPDYSEFQSHPYHRASSRQPDYPGSQRNPDFAGSSSSGNYAGSRTHPDHFGSLEPDYPGAQSNSDHPGPRANLNHPGSRKNLEHTSFRINPYADSLGKPDYPGADIQPNSPPFFGEPDYPSAEDNQNLPSTWREPDYSDAENGHDYGSSETPKMTRGVLSRTSSIQPSFRHRSDDPVGSLWGENDYPEGIEMASMEMANSYGHSLPGAPGSGYVNPAYVGESGPVHAYGNPPLSECDWHKSPQGQKLIASLIPMTSRDRIKAIRNQPRTMEEKRNLRKIVDKEKSKQTHRILQLNCCIQCLNSISRAYRRSKNSLSEILNSISLWQKTLKIIGGKFGTSVLSYFNFLRWLLKFNIFSFILNFSFIIIPQFTVAKKNTLQFTGLEFFTGVGYFRDTVMYYGFYTNSTIQHGNSGASYNMQLAYIFTIGACLTTCFFSLLFSMAKYFRNNFINPHIYSGGITKLIFCWDFTVTHEKAVKLKQKNLSTEIRENLSELRQENSKLTFNQLLTRFSAYMVAWVVSTGVAIACCAAVYYLAEYNLEFLKTHSNPGAVLLLPFVVSCINLAVPCIYSMFRLVERYEMPRHEVYVLLIRNIFLKISIIGILCYYWLNTVALSGEECWETLIGQDIYRLLLMDFVFSLVNSFLGEFLRRIIGMQLITSLGLQEFDIARNVLELIYAQTLVWIGIFFCPLLPFIQMIMLFIMFYSKNISLMMNFQPPSKAWRASQMMTFFIFLLFFPSFTGVLCTLAITIWRIITYLYWQITEGRKIMIRLLHEQIINEGKDKMFLIEKLIKLQDMEKKANPSSLVLERREVEQQGFLHLGEHDGSLDLRSRRSVQEGNPRA
維真生物(wu)的人源cDNA克隆中(zhong),雖然我們大約70%的克隆與RefSeq或GeneBank所發表的基因(yin)列序完(wan)全一致,但也有些(xie)克隆含有天然的單核苷酸多態(tai)性(xing)而包含一至多個SNPs, 因此與RefSeq或GeneBank所發表的列序會(hui)有些不同。我們強烈建(jian)議您在購買之前(qian)仔細將我(wo)們(men)克隆的序(xu)列與您所要求(qiu)的序(xu)列進(jin)行對比,以(yi)決定所購買的(de)克隆是否符合您(nin)的(de)要(yao)(yao)求。如果我們(men)預制的(de)克隆不符合您(nin)的(de)要(yao)(yao)求,我們(men)一般在1-2周內可以提供一(yi)(yi)(yi)個(ge)與(yu)您所要(yao)求的序列(lie)完(wan)全(quan)一(yi)(yi)(yi)致(zhi)的克隆。我們(men)的每一(yi)(yi)(yi)個(ge)克隆與(yu)RefSeq(2013)標準序(xu)列相比都(dou)含有(you)一個完整的ORF(Opening Reading Frame)。因為(wei)RefSeq數據庫會不(bu)斷更(geng)新,更(geng)新后的標準序(xu)列(lie)將來有可能與(yu)我們(men)現有的克(ke)隆序(xu)列(lie)產生差異。我們(men)所有的克(ke)隆產品(pin)都經(jing)過二(er)代(NextGen)測序驗證,并(bing)且保證網站(zhan)上克隆(long)的(de)序列與您收到的(de)克隆(long)序列完(wan)全一致。在未經維真生物書面給予授權前,人源(yuan)ORF cDNA克隆不(bu)可轉售或贈予買方(fang)以外的(de)第三方(fang)。同(tong)時, 在使(shi)用任(ren)何維(wei)真(zhen)產(chan)品(pin)之前,買方(fang)有責任確認該(gai)基因的(de)(de)使用不(bu)會侵犯(fan)到第三方(fang)的(de)(de)知(zhi)識產(chan)權。此產(chan)品專(zhuan)門為基礎研究所開發,不(bu)適(shi)用于(yu)任何治(zhi)療或診斷應用。