產(chan)品名(ming)稱:人源(yuan)cDNA克隆
產品規格:500μL甘油菌液
產品運輸條件:常溫
產品(pin)儲存(cun)條件(jian)和(he)期限(xian):-80℃長期保存
產品特點:
? 人源基因C-端融合(he) Flag & 6×His 標(biao)簽;
? 含(han)有(you)puromycin真核抗性基因,可篩(shai)選(xuan)穩定轉染細胞株;
? 含有卡那霉素原核抗(kang)性基因,可篩選重組克(ke)隆(long),推(tui)薦使用濃度為30μg/ml。
產(chan)品使(shi)用:
將(jiang)甘油(you)菌(jun)在新(xin)鮮配制的固體培養基(相應抗性)中(zhong)劃線,37℃培(pei)養箱培(pei)養過夜,挑取3個單(dan)克(ke)隆(long),分別(bie)接種(zhong)于相應抗性的液體培(pei)養基中,37℃搖床培(pei)養過夜,然后進行(xing)質粒(li)的提取和驗(yan)證(zheng)。
溫馨提示(shi):考慮到實驗的嚴謹性,建議您(nin)拿到產品的第(di)一時(shi)間進行(xing)測序確認。
ATGGATCGCAGCTCCAAGAGGAGGCAGGTGAAGCCTTTGGCAGCTTCTCTGCTGGAAGCTCTTGATTATGATAGTTCAGATGACAGTGATTTTAAAGTTGGAGATGCCTCAGATTCTGAAGGGAGTGGTAATGGAAGTGAAGATGCTTCAAAGGACAGTGGAGAAGGTTCCTGTAGTGATTCTGAAGAAAATATTTTAGAAGAAGAACTGAATGAAGATATTAAAGTAAAAGAAGAACAACTTAAAAATTCTGCAGAGGAAGAAGTACTATCATCAGAAAAACAATTAATTAAAATGGAAAAGAAGGAAGAAGAAGAAAATGGAGAAAGACCTAGAAAGAAAAAGGAGAAAGAGAAGGAAAAAGAAAAGGAAAAGGAGAAAGAGAAGGAAAGAGAGAAGGAAAAAGAAAAAGCAACAGTATCTGAGAATGTGGCTGCTTCTGCTGCTGCCACCACACCAGCCACAAGTCCTCCTGCTGTTAACACATCCCCTTCTGTTCCCACTACGACAACCGCTACAGAGGAACAAGTCAGCGAGCCAAAAAAATGGAACCTTCGACGAAACCGACCACTTCTGGATTTTGTGTCCATGGAAGAGCTGAATGACATGGATGACTATGACAGTGAGGATGACAATGATTGGCGACCTACTGTAGTAAAGAGAAAAGGGAGATCTGCGTCTCAGAAAGAGGGAAGTGATGGAGACAATGAGGATGATGAAGATGAGGGAAGCGGGAGTGATGAAGACGAGAATGATGAAGGCAATGATGAAGATCATAGTAGCCCTGCCAGTGAAGGGGGTTGCAAGAAGAAGAAGAGTAAAGTTCTTAGCAGAAACAGTGCTGATGATGAGGAACTGACCAATGATAGCCTGACCCTATCTCAAAGCAAGAGTAATGAGGACTCGCTGATTCTTGAGAAGAGTCAAAACTGGAGCTCTCAAAAAATGGACCATATTCTGATTTGCTGTGTTTGTCTGGGAGATAATAGTGAGGACGCTGATGAAATAATTCAGTGTGACAATTGTGGCATTACAGTCCATGAAGGTTGTTATGGAGTTGATGGAGAGAGTGACTCTATTATGAGTTCAGCTTCTGAAAACTCCACTGAACCTTGGTTTTGTGATGCCTGTAAATGTGGTGTTTCTCCTAGCTGTGAACTGTGTCCTAATCAGGATGGAATTTTCAAGGAGACAGATGCTGGAAGATGGGTTCATATTGTTTGTGCCCTGTATGTTCCTGGAGTAGCCTTTGGAGATATTGACAAATTACGACCAGTAACACTAACGGAAATGAACTATTCCAAATATGGTGCCAAGGAGTGTAGCTTTTGTGAAGACCCTCGCTTTGCTAGAACTGGGGTTTGCATTAGCTGTGATGCAGGGATGTGCAGAGCCTATTTCCATGTGACCTGTGCTCAAAAGGAAGGTCTGCTTTCAGAGGCAGCGGCGGAAGAGGATATAGCAGATCCATTCTTTGCTTATTGTAAGCAACATGCAGATAGGTTAGACAGAAAGTGGAAGAGAAAAAACTACTTGGCTCTACAGTCCTATTGTAAAATGTCTTTGCAAGAGAGAGAGAAGCAACTATCACCAGAAGCACAGGCAAGGATCAATGCCCGGCTTCAGCAGTATCGTGCCAAAGCAGAACTAGCTCGATCTACCAGACCCCAGGCCTGGGTTCCAAGGGAAAAATTGCCCAGACCACTCACCAGCAGTGCTTCAGCTATTCGTAAACTTATGCGGAAAGCAGAACTCATGGGGATCAGTACAGATATCTTTCCAGTGGACAATTCAGATACTAGTTCTAGTGTGGATGGAAGGAGAAAACATAAGCAACCAGCTCTCACTGCAGATTTTGTGAATTATTATTTTGAGAGAAATATGCGCATGATTCAAATTCAGGAAAATATGGCTGAACAAAAGAATATAAAAGATAAATTAGAGAATGAACAAGAAAAGCTTCATGTAGAATATAATAAGCTATGTGAATCTTTAGAAGAACTACAAAACCTGAATGGAAAACTTCGAAGTGAAGGACAAGGAATATGGGCTTTACTAGGCAGAATCACAGGGCAGAAGTTGAATATACCGGCAATTTTGCGAGCACCCAAGGAGAGAAAACCAAGTAAAAAAGAAGGAGGCACACAAAAGACATCTACTCTTCCTGCAGTACTTTATAGTTGTGGGATTTGTAAGAAGAACCATGATCAGCATCTTCTTTTATTGTGTGATACCTGTAAACTACATTACCATCTTGGATGTCTGGATCCTCCTCTTACAAGGATGCCAAGAAAGACCAAAAACAGTTATTGGCAGTGCTCGGAATGTGACCAGGCAGGGAGCAGTGACATGGAAGCAGATATGGCCATGGAAACCCTACCAGATGGAACCAAACGATCAAGGAGGCAGATTAAGGAACCAGTGAAATTTGTTCCACAGGATGTGCCACCAGAACCCAAGAAGATTCCGATAAGAAACACGAGAACCAGAGGACGAAAACGAAGCTTCGTTCCTGAGGAAGAAAAACATGAGGAAAGAGTTCCTAGAGAGAGAAGACAAAGACAGTCTGTGTTGCAAAAGAAGCCCAAGGCTGAAGATTTAAGAACTGAATGTGCAACTTGCAAGGGAACTGGAGACAATGAAAATCTTGTCAGATACCCTTCA
MDRSSKRRQVKPLAASLLEALDYDSSDDSDFKVGDASDSEGSGNGSEDASKDSGEGSCSDSEENILEEELNEDIKVKEEQLKNSAEEEVLSSEKQLIKMEKKEEEENGERPRKKKEKEKEKEKEKEKEKEREKEKEKATVSENVAASAAATTPATSPPAVNTSPSVPTTTTATEEQVSEPKKWNLRRNRPLLDFVSMEELNDMDDYDSEDDNDWRPTVVKRKGRSASQKEGSDGDNEDDEDEGSGSDEDENDEGNDEDHSSPASEGGCKKKKSKVLSRNSADDEELTNDSLTLSQSKSNEDSLILEKSQNWSSQKMDHILICCVCLGDNSEDADEIIQCDNCGITVHEGCYGVDGESDSIMSSASENSTEPWFCDACKCGVSPSCELCPNQDGIFKETDAGRWVHIVCALYVPGVAFGDIDKLRPVTLTEMNYSKYGAKECSFCEDPRFARTGVCISCDAGMCRAYFHVTCAQKEGLLSEAAAEEDIADPFFAYCKQHADRLDRKWKRKNYLALQSYCKMSLQEREKQLSPEAQARINARLQQYRAKAELARSTRPQAWVPREKLPRPLTSSASAIRKLMRKAELMGISTDIFPVDNSDTSSSVDGRRKHKQPALTADFVNYYFERNMRMIQIQENMAEQKNIKDKLENEQEKLHVEYNKLCESLEELQNLNGKLRSEGQGIWALLGRITGQKLNIPAILRAPKERKPSKKEGGTQKTSTLPAVLYSCGICKKNHDQHLLLLCDTCKLHYHLGCLDPPLTRMPRKTKNSYWQCSECDQAGSSDMEADMAMETLPDGTKRSRRQIKEPVKFVPQDVPPEPKKIPIRNTRTRGRKRSFVPEEEKHEERVPRERRQRQSVLQKKPKAEDLRTECATCKGTGDNENLVRYPS
維真生物的人源cDNA克(ke)隆中,雖然(ran)我們大約70%的(de)克隆與RefSeq或(huo)GeneBank所發表(biao)的基因(yin)列(lie)序完全一致,但也(ye)有些克隆含有天然的單核苷酸多態性(xing)而(er)包含一(yi)至(zhi)多個SNPs, 因此與RefSeq或GeneBank所(suo)發表的列序會有些(xie)不(bu)同。我們強烈建議您在(zai)購買之前仔細將我們(men)克隆的(de)序列(lie)(lie)與您所(suo)要求的(de)序列(lie)(lie)進行(xing)對(dui)比,以決(jue)定所購買(mai)的克隆是否(fou)符合(he)您的要(yao)求。如果我們(men)預(yu)制的克隆不符合(he)您的要(yao)求,我們(men)一般在1-2周(zhou)內(nei)可以提供一(yi)個與(yu)您(nin)所要求的(de)序列完全一(yi)致(zhi)的(de)克(ke)隆。我們的(de)每一(yi)個克(ke)隆與(yu)RefSeq(2013)標(biao)準序列相比都含有一個完整的(de)ORF(Opening Reading Frame)。因(yin)為RefSeq數據(ju)庫會不斷(duan)更(geng)新,更(geng)新后的標準序列(lie)將來有(you)可能與(yu)我們(men)(men)現(xian)有(you)的克隆序列(lie)產生差(cha)異。我們(men)(men)所有(you)的克隆產品都經過(guo)二代(NextGen)測序(xu)驗證,并且保證網站上克隆的序(xu)列與(yu)您收到的克隆序(xu)列完全一致。在(zai)未經維真(zhen)生物(wu)書面給予授(shou)權前,人源ORF cDNA克(ke)隆不可轉售(shou)或贈予買方(fang)以外的(de)第三方(fang)。同時, 在使(shi)用任何維真(zhen)產品之前,買方有責任(ren)確(que)認該基因的使用(yong)不(bu)會侵犯到(dao)第三方的知識(shi)產(chan)權。此產(chan)品專(zhuan)門為基礎研究所開發,不(bu)適(shi)用(yong)于任(ren)何治療(liao)或診斷應用(yong)。