產品名(ming)稱(cheng):人源cDNA克隆
產品(pin)規格:500μL甘油菌液
產品運(yun)輸條件:常溫
產(chan)品儲(chu)存條(tiao)件和(he)期(qi)限(xian):-80℃長(chang)期(qi)保(bao)存
產(chan)品特點(dian):
? 人源基因C-端融合 Flag & 6×His 標簽(qian);
? 含有puromycin真核抗(kang)性基因,可篩選穩(wen)定(ding)轉染細胞株(zhu);
? 含有(you)卡那霉素原核抗性(xing)基因,可(ke)篩選重組(zu)克隆,推薦使(shi)用濃(nong)度為(wei)30μg/ml。
產品使用:
將甘(gan)油菌在新(xin)鮮配制的固體(ti)培養基(相應抗性(xing))中劃線,37℃培(pei)(pei)養(yang)箱培(pei)(pei)養(yang)過(guo)夜,挑取3個單克隆,分別接種于相應抗性的液體培(pei)(pei)養(yang)基中,37℃搖床培(pei)(pei)養(yang)過(guo)夜,然后進行質粒的提取和(he)驗(yan)證。
溫馨提示(shi):考慮到實驗(yan)的嚴謹性,建議您拿到產品(pin)的第(di)一時間進行測序確認。
ATGGCCCGGGGCGCCCGGCCCTCGGCGGCCGGTGGCGGCGGCGGCGGCGCGGAGCCCCCTGAGCGCGCGGGCCCCGGGCGGCCGCGGGGATCCCCGCCCGGCCGCGCCCGCCCCTCGCTGGCGCCGCGCCCCGGCCCGGAGCCCTCGCGACCCCGGGCGGCGCCCGAGACCTCCGGCGGGGACACGGCGGGCGCGGGGCGGTGCGGCGGGCGGCGGGCGGCAAAGTTGGGGCCGGGCCGCCGCGGCTGGTGGGCGCTGCTGGCGCTGCAGCTGCACTTGCTCCGGGCGCTGGCGCAAGATGATGTTGCTCCATATTTTAAAACGGAGCCAGGCCTACCACAGATCCACCTGGAAGGGAACCGCCTTGTTCTCACCTGCCTTGCCGAAGGGAGCTGGCCTTTGGAGTTCAAGTGGATGCGCGATGACAGTGAGCTCACCACCTACAGCAGCGAATATAAGTACATTATTCCATCTTTGCAGAAGCTCGATGCTGGGTTTTACCGCTGCGTGGTGCGAAACAGAATGGGAGCACTCCTGCAAAGAAAATCAGAAGTTCAAGTCGCATATATGGGAAGTTTCATGGATACGGACCAGAGGAAAACAGTTTCTCAAGGACGTGCAGCGATTCTAAACCTGCTGCCCATCACCAGCTACCCCAGACCTCAAGTGACTTGGTTTAGAGAAGGGCACAAGATTATTCCAAGCAACAGAATAGCCATCACATTGGAGAATCAGCTGGTGATCCTCGCCACCACAACCAGTGATGCCGGGGCATACTACGTGCAGGCCGTGAATGAGAAAAATGGAGAAAACAAGACAAGCCCATTCATTCATTTGAGCATAGCAAGAGATGTTGGCACACCTGAAACCATGGCCCCAACCATTGTGGTTCCCCCGGGCAACAGAAGTGTGGTGGCTGGATCCAGTGAGACCACCTTGGAATGTATAGCCAGTGCCAGGCCTGTGGAGGACCTGAGTGTGACCTGGAAGAGGAATGGAGTGAGAATCACCAGTGGCCTCCACAGCTTTGGAAGACGCCTCACCATCAGCAACCCGACGTCCGCGGACACCGGGCCATACGTCTGCGAGGCGGCGCTGCCGGGGAGCGCTTTTGAACCGGCCAGGGCGACGGCCTTTCTTTTCATCATAGAGCCACCATATTTTACTGCTGAGCCCGAGAGTCGGATTTCAGCTGAAGTAGAAGAAACTGTGGACATCGGATGTCAAGCCATGGGGGTCCCCCTTCCCACCCTCCAGTGGTACAAGGATGCCATCTCCATCAGCAGGCTCCAGAATCCTCGATACAAAGTGCTCGCCAGCGGAGGCCTGCGCATCCAGAAGCTGCGTCCAGAGGACTCCGGAATCTTCCAGTGCTTCGCCAGCAATGAAGGAGGGGAGATCCAGACCCACACCTACCTGGATGTAACCAATATCGCTCCAGTGTTCACCCAGCGGCCAGTGGACACCACAGTTACTGACGGGATGACAGCCATTCTAAGGTGTGAGGTGTCCGGGGCTCCCAAACCCGCCATCACCTGGAAAAGAGAAAACCACATTCTGGCCAGTGGCTCTGTCCGGATTCCTAGGTTCATGCTTCTTGAATCGGGGGGTCTACAGATCGCGCCCGTCTTCATCCAGGATGCCGGCAACTACACCTGCTATGCGGCCAACACAGAGGGCTCCCTGAATGCATCGGCCACGCTCACTGTGTGGAATCGGACGTCCATCGTCCACCCTCCTGAGGACCACGTGGTGATTAAGGGGACCACGGCCACGCTGCACTGTGGTGCCACACATGACCCCCGGGTTTCACTCCGCTACGTTTGGAAGAAGGACAACGTGGCCCTGACTCCATCGAGCACGTCTAGGATCGTGGTGGAGAAGGACGGGTCCCTTCTCATCAGCCAGACGTGGTCAGGCGACATCGGTGACTACAGCTGCGAGATTGTTTCTGAAGGAGGGAATGACTCCAGGATGGCCCGGCTGGAAGTGATTGAACTGCCTCATTCACCTCAGAACCTCCTGGTCAGCCCTAATTCTTCCCACAGCCACGCCGTGGTGCTCTCTTGGGTCCGGCCCTTTGATGGAAACAGTCCTATTCTTTATTACATCGTGGAGCTCTCTGAAAACAACTCTCCATGGAAGGTGCATCTGTCAAACGTTGGCCCTGAGATGACAGGCGTCACCGTGAGTGGCCTGACTCCGGCTCGTACCTATCAATTCCGGGTGTGCGCGGTGAATGAAGTGGGCAGGGGCCAGTACAGCGCCGAGACAAGCAGGTTGATGCTACCTGAAGAACCACCCAGTGCTCCCCCGAAAAATATAGTGGCCAGTGGGCGGACTAATCAGTCCATTATGGTCCAGTGGCAGCCACCCCCAGAAACAGAGCACAACGGGGTGTTGCGTGGATACATCCTCAGGTACCGCCTGGCTGGCCTTCCCGGAGAGTACCAGCAGCGGAACATCACCAGCCCGGAGGTGAACTACTGCCTGGTGACAGACCTGATCATCTGGACACAGTATGAGATACAGGTGGCGGCGTACAACGGGGCCGGTCTGGGCGTCTTCAGCAGGGCAGTGACCGAGTACACCTTGCAGGGAGTGCCCACCGCGCCCCCGCAGAACGTGCAGACGGAAGCCGTGAACTCCACCACCATTCAGTTCCTGTGGAACCCTCCGCCTCAGCAGTTTATCAATGGCATCAACCAGGGATACAAGCTTCTGGCATGGCCGGCAGATGCCCCCGAGGCTGTCACTGTGGTCACTATTGCCCCAGATTTCCACGGAGTCCACCATGGACACATAACGAACCTGAAGAAGTTTACCGCCTACTTCACTTCCGTTCTGTGCTTCACCACCCCTGGGGACGGGCCTCCCAGCACACCTCAGCTGGTCTGGACTCAGGAAGACAAACCAGGAGCTGTGGGACATCTGAGTTTCACAGAGATCTTGGACACATCTCTCAAGGTCAGCTGGCAGGAGCCCCTGGAGAAAAATGGCATCATTACTGGCTATCAGATCTCTTGGGAAGTGTACGGCAGGAACGACTCTCGTCTCACGCACACCCTGAACAGCACGACGCACGAGTACAAGATCCAAGGCCTCTCATCTCTCACCACCTACACCATCGACGTGGCCGCTGTGACTGCCGTGGGCACTGGCCTGGTGACTTCATCCACCATTTCTTCTGGAGTGCCCCCAGACCTTCCTGGTGCCCCATCCAACCTGGTCATTTCCAACATCAGCCCTCGCTCCGCCACCCTTCAGTTCCGGCCAGGCTATGACGGGAAAACGTCCATCTCCAGGTGGATTGTTGAGGGGCAGGTGGGAGCTATCGGCGACGAGGAGGAGTGGGTCACCCTCTATGAAGAGGAGAATGAGCCTGATGCCCAGATGCTGGAGATCCCAAACCTCACACCCTACACTCACTACAGATTTCGAATGAAGCAAGTGAACATTGTTGGGCCGAGCCCCTACAGTCCGTCTTCCCGGGTCATCCAGACCCTGCAGGCCCCACCCGACGTGGCTCCAACCAGCGTCACGGTCCGTACTGCCAGTGAGACCAGCCTGCGGCTTCGCTGGGTGCCCCTGCCGGATTCTCAGTACAACGGGAACCCCGAGTCCGTGGGCTACAGGATTAAGTACTGGCGCTCAGACCTCCAGTCCTCAGCAGTGGCCCAAGTCGTCAGTGACCGGCTGGAGAGAGAATTCACCATCGAGGAGCTGGAGGAGTGGATGGAATACGAGCTGCAGATGCAGGCCTTCAACGCCGTCGGGGCTGGGCCGTGGAGCGAGGTGGTGCGGGGCCGGACGCGGGAGTCAGTTCCTTCAGCCGCCCCTGAGAACGTGTCAGCCGAGGCTGTCAGCTCGACCCAGATTTTACTGACATGGACATCCGTGCCGGAACAGGACCAGAATGGGCTCATACTGGGCTACAAGATCCTGTTCCGGGCCAAAGACCTGGATCCCGAGCCCAGGAGCCACATCGTGCGAGGGAACCACACGCAGTCGGCCCTGCTGGCAGGCCTGCGCAAGTTCGTGCTCTACGAGCTCCAGGTGCTGGCGTTCACCCGCATCGGGAACGGGGTCCCCAGCACGCCCCTCATCCTGGAGCGCACCAAAGACGATGCCCCAGGCCCACCAGTGAGGCTCGTGTTCCCCGAAGTGAGACTCACCTCCGTGCGGATAGTGTGGCAACCTCCGGAGGAGCCCAACGGCATCATCCTGGGGTACCAGATTGCCTACCGCCTGGCCAGCAGCAGCCCCCACACCTTCACCACCGTGGAGGTCGGCGCCACAGTGAGGCAGTTCACAGCCACCGACCTGGCCCCGGAGTCCGCATACATCTTCAGGCTGTCCGCCAAGACGAGGCAGGGCTGGGGGGAGCCACTGGAGGCCACCGTCATCACCACCGAGAAGAGAGAGCGGCCGGCACCCCCCAGAGAGCTCCTGGTGCCCCAGGCAGAAGTGACCGCACGCAGCCTCCGGCTCCAGTGGGTCCCGGGCAGCGACGGGGCCTCCCCCATCCGGTACTTCACCATGCAGGTGCGAGAGCTGCCTCGGGGTGAGTGGCAGACCTACTCCTCGTCCATCAGCCATGAGGCGACAGCATGCGTCGTTGACAGACTGAGGCCCTTCACCTCCTACAAGCTGCGCCTGAAAGCCACCAACGACATTGGGGACAGTGACTTCAGTTCAGAGACAGAGGCGGTGACCACGCTGCAGGATGTTCCAGGAGAGCCCCCGGGATCTGTCTCAGCGACGCCACACACCACGTCCTCTGTCCTGATACAGTGGCAGCCTCCGAGGGACGAAAGCCTGAATGGCCTTCTTCAGGGATACAGGATCTACTACAGGGAGCTGGAGTATGAAGCCGGGTCAGGCACTGAGGCCAAGACGCTCAAAAACCCTATAGCTTTACATGCTGAGCTCACAGCCCAAAGCAGCTTCAAGACGGTGAACAGCAGCTCCACATCGACGATGTGTGAACTAACACATTTAAAGAAGTACCGGCGCTATGAAGTAATAATGACCGCCTATAACATCATCGGCGAGAGCCCAGCCAGCGCGCCCGTGGAGGTCTTTGTCGGCGAGGCTGCCCCGGCCATGGCCCCGCAGAACGTGCAGGTGACCCCACTCACGGCCAGCCAGCTGGAGGTCACGTGGGACCCACCACCCCCGGAGAGCCAGAATGGGAACATCCAAGGCTACAAGATTTACTACTGGGAGGCAGACAGCCAGAACGAAACGGAGAAAATGAAGGTCCTCTTCCTCCCCGAGCCCGTGGTGAGGCTGAAGAACCTGACCAGCCATACCAAGTACCTGGTCAGCATATCAGCCTTCAACGCCGCCGGAGATGGACCTAAGAGTGACCCCCAGCAGGGGCGCACCCACCAGGCCGCCCCTGGGGCCCCCAGCTTTCTGGCGTTCTCAGAAATAACCTCCACCACGCTCAACGTGTCCTGGGGCGAGCCTGCGGCGGCCAACGGCATCCTGCAGGGCTATCGGGTGGTGTACGAGCCCTTGGCCCCTGTACAAGGGGTGAGCAAGGTGGTGACCGTGGAAGTGAGAGGGAACTGGCAGCGCTGGCTGAAGGTGCGGGACCTCACCAAGGGAGTGACCTATTTCTTCCGTGTCCAAGCGCGGACCATCACCTACGGGCCCGAGCTCCAAGCCAATATCACAGCCGGGCCAGCCGAGGGATCCCCGGGCTCGCCTAGAGATGTCCTGGTCACCAAGTCCGCCTCTGAACTGACGCTGCAGTGGACTGAGGGACACTCTGGCGACACACCTACCACGGGCTATGTGATCGAGGCCCGGCCCTCAGATGAAGGCTTATGGGACATGTTTGTGAAGGACATCCCGCGGAGCGCCACATCCTACACCCTCAGCCTGGATAAGCTCCGGCAAGGAGTGACTTACGAGTTCCGGGTGGTGGCTGTGAATGAGGCGGGCTACGGGGAGCCCAGCAACCCCTCCACGGCTGTGTCAGCTCAAGTGGAAGCCCCATTCTACGAGGAGTGGTGGTTCCTCCTGGTGATGGCTCTGTCCAGCCTGATCGTCATCCTGCTGGTGGTGTTCGCCCTCGTCCTGCACGGGCAGAATAAGAAGTATAAGAACTGCAGCACAGGAAAGGGGATCTCCACCATGGAGGAGTCTGTGACCCTGGACAACGGAGGATTTGCTGCCCTGGAGCTCAGCAGCCGCCACCTCAATGTCAAGAGCACCTTCTCCAAGAAGAACGGGACCAGGTCCCCACCCCGGCCTAGCCCCGGCGGCCTGCACTACTCAGACGAGGACATCTGCAACAAGTACAACGGCGCCGTGCTGACCGAGAGCGTGAGCCTCAAGGAGAAGTCGGCAGATGCATCAGAATCTGAGGCCACGGACTCTGACTACGAGGACGCGCTGCCCAAGCACTCCTTCGTGAACCACTACATGAGCGACCCCACCTACTACAACTCATGGAAGCGCAGGGCCCAGGGCCGCGCACCTGCGCCGCACAGGTACGAGGCGGTGGCGGGCTCCGAGGCGGGCGCGCAGCTGCACCCGGTCATCACCACGCAGAGCGCGGGCGGCGTCTACACCCCCGCTGGCCCCGGCGCGCGAACTCCGCTCACCGGCTTCTCCTCCTTCGTG
MARGARPSAAGGGGGGAEPPERAGPGRPRGSPPGRARPSLAPRPGPEPSRPRAAPETSGGDTAGAGRCGGRRAAKLGPGRRGWWALLALQLHLLRALAQDDVAPYFKTEPGLPQIHLEGNRLVLTCLAEGSWPLEFKWMRDDSELTTYSSEYKYIIPSLQKLDAGFYRCVVRNRMGALLQRKSEVQVAYMGSFMDTDQRKTVSQGRAAILNLLPITSYPRPQVTWFREGHKIIPSNRIAITLENQLVILATTTSDAGAYYVQAVNEKNGENKTSPFIHLSIARDVGTPETMAPTIVVPPGNRSVVAGSSETTLECIASARPVEDLSVTWKRNGVRITSGLHSFGRRLTISNPTSADTGPYVCEAALPGSAFEPARATAFLFIIEPPYFTAEPESRISAEVEETVDIGCQAMGVPLPTLQWYKDAISISRLQNPRYKVLASGGLRIQKLRPEDSGIFQCFASNEGGEIQTHTYLDVTNIAPVFTQRPVDTTVTDGMTAILRCEVSGAPKPAITWKRENHILASGSVRIPRFMLLESGGLQIAPVFIQDAGNYTCYAANTEGSLNASATLTVWNRTSIVHPPEDHVVIKGTTATLHCGATHDPRVSLRYVWKKDNVALTPSSTSRIVVEKDGSLLISQTWSGDIGDYSCEIVSEGGNDSRMARLEVIELPHSPQNLLVSPNSSHSHAVVLSWVRPFDGNSPILYYIVELSENNSPWKVHLSNVGPEMTGVTVSGLTPARTYQFRVCAVNEVGRGQYSAETSRLMLPEEPPSAPPKNIVASGRTNQSIMVQWQPPPETEHNGVLRGYILRYRLAGLPGEYQQRNITSPEVNYCLVTDLIIWTQYEIQVAAYNGAGLGVFSRAVTEYTLQGVPTAPPQNVQTEAVNSTTIQFLWNPPPQQFINGINQGYKLLAWPADAPEAVTVVTIAPDFHGVHHGHITNLKKFTAYFTSVLCFTTPGDGPPSTPQLVWTQEDKPGAVGHLSFTEILDTSLKVSWQEPLEKNGIITGYQISWEVYGRNDSRLTHTLNSTTHEYKIQGLSSLTTYTIDVAAVTAVGTGLVTSSTISSGVPPDLPGAPSNLVISNISPRSATLQFRPGYDGKTSISRWIVEGQVGAIGDEEEWVTLYEEENEPDAQMLEIPNLTPYTHYRFRMKQVNIVGPSPYSPSSRVIQTLQAPPDVAPTSVTVRTASETSLRLRWVPLPDSQYNGNPESVGYRIKYWRSDLQSSAVAQVVSDRLEREFTIEELEEWMEYELQMQAFNAVGAGPWSEVVRGRTRESVPSAAPENVSAEAVSSTQILLTWTSVPEQDQNGLILGYKILFRAKDLDPEPRSHIVRGNHTQSALLAGLRKFVLYELQVLAFTRIGNGVPSTPLILERTKDDAPGPPVRLVFPEVRLTSVRIVWQPPEEPNGIILGYQIAYRLASSSPHTFTTVEVGATVRQFTATDLAPESAYIFRLSAKTRQGWGEPLEATVITTEKRERPAPPRELLVPQAEVTARSLRLQWVPGSDGASPIRYFTMQVRELPRGEWQTYSSSISHEATACVVDRLRPFTSYKLRLKATNDIGDSDFSSETEAVTTLQDVPGEPPGSVSATPHTTSSVLIQWQPPRDESLNGLLQGYRIYYRELEYEAGSGTEAKTLKNPIALHAELTAQSSFKTVNSSSTSTMCELTHLKKYRRYEVIMTAYNIIGESPASAPVEVFVGEAAPAMAPQNVQVTPLTASQLEVTWDPPPPESQNGNIQGYKIYYWEADSQNETEKMKVLFLPEPVVRLKNLTSHTKYLVSISAFNAAGDGPKSDPQQGRTHQAAPGAPSFLAFSEITSTTLNVSWGEPAAANGILQGYRVVYEPLAPVQGVSKVVTVEVRGNWQRWLKVRDLTKGVTYFFRVQARTITYGPELQANITAGPAEGSPGSPRDVLVTKSASELTLQWTEGHSGDTPTTGYVIEARPSDEGLWDMFVKDIPRSATSYTLSLDKLRQGVTYEFRVVAVNEAGYGEPSNPSTAVSAQVEAPFYEEWWFLLVMALSSLIVILLVVFALVLHGQNKKYKNCSTGKGISTMEESVTLDNGGFAALELSSRHLNVKSTFSKKNGTRSPPRPSPGGLHYSDEDICNKYNGAVLTESVSLKEKSADASESEATDSDYEDALPKHSFVNHYMSDPTYYNSWKRRAQGRAPAPHRYEAVAGSEAGAQLHPVITTQSAGGVYTPAGPGARTPLTGFSSFV
維真生物的人源cDNA克隆中,雖然我們大約70%的克(ke)隆與(yu)RefSeq或GeneBank所發表(biao)的基因列序完全(quan)一致(zhi),但也有些克隆含(han)有天然的單(dan)核苷(gan)酸(suan)多態性而包含(han)一至多個SNPs, 因此(ci)與RefSeq或GeneBank所發表(biao)的列(lie)序會有(you)些(xie)不同(tong)。我們強烈建議您在(zai)購買之前仔細將我們克隆的(de)序列與您所要求的(de)序列進行對(dui)比,以(yi)決定所購買的(de)(de)克隆是否符合您(nin)(nin)的(de)(de)要求。如果我們(men)預制的(de)(de)克隆不符合您(nin)(nin)的(de)(de)要求,我們(men)一般在1-2周內可以提(ti)供一個(ge)與您(nin)所要求的序(xu)列完全一致的克隆。我(wo)們的每一個(ge)克隆與RefSeq(2013)標準序列相比都含有一個完整的(de)ORF(Opening Reading Frame)。因為RefSeq數據庫會不斷(duan)更新(xin),更新(xin)后(hou)的標(biao)準序列(lie)將來有可(ke)能與我們(men)現有的克(ke)(ke)隆(long)序列(lie)產生(sheng)差異。我們(men)所有的克(ke)(ke)隆(long)產品都經(jing)過二代(NextGen)測(ce)序驗證(zheng),并且保證(zheng)網(wang)站上克隆的序列(lie)與您收到的克隆序列(lie)完全一致(zhi)。在(zai)未經維真生物(wu)書面給(gei)予授權前,人(ren)源ORF cDNA克隆不可轉售或贈予買方以外的第三方。同時, 在使用任(ren)何維真(zhen)產品之前,買方(fang)有責任確(que)認(ren)該基(ji)因的使用不(bu)會侵(qin)犯到(dao)第三方(fang)的知識產(chan)權。此產(chan)品專門為基(ji)礎研究所開發,不(bu)適用于任何(he)治療或診斷(duan)應用。