基因編號: NM_182490
產品編號: CH805989
基因名稱: ZNF227;
基因大小: 2400 bp
分子量: 88 kDa
酶切位點: SgfI_MluI
價格: 799
供貨時間: next day
產品名稱：人源cDNA克(ke)隆
產品規格：500μL甘油菌液
產品運輸條件：常溫
產品儲存條件和期限：-80℃長期保存
產品特點：
? 人源基因C-端(duan)融合 Flag & 6×His 標簽；
? 含(han)有puromycin真核抗性基(ji)因，可篩選穩定轉(zhuan)染細胞株；
? 含有卡那霉(mei)素(su)原核抗性基因，可篩選重組(zu)克隆，推薦使用濃度為30μg/ml。
產品(pin)使用(yong)：
將(jiang)甘(gan)油(you)菌在新(xin)鮮(xian)配制的固體培養(yang)基（相(xiang)應抗性）中(zhong)劃線，37℃培(pei)養(yang)(yang)箱培(pei)養(yang)(yang)過夜(ye)(ye)，挑(tiao)取(qu)3個單克隆，分別接種于相應抗性(xing)的(de)液體培(pei)養(yang)(yang)基中，37℃搖床培(pei)養(yang)(yang)過夜(ye)(ye)，然后(hou)進(jin)行質粒(li)的(de)提取(qu)和驗證。
溫馨(xin)提示：考慮到實驗(yan)的嚴謹性，建議您拿到產品的(de)第一時間(jian)進行測序(xu)確(que)認(ren)。
CGCCATGCCATCTCAGAACTATGACCTTCCCCAGAAGAAGCAGGAGAAAATGACCAAGTTTCAGGAGGCTGTGACATTCAAGGATGTGGCTGTGGTCTTCTCCAGGGAGGAACTGCGACTGCTCGATCTTACCCAGAGGAAGCTGTACCGAGATGTCATGGTGGAGAACTTCAAGAACCTGGTTGCAGTGGGGCATCTTCCCTTCCAACCAGATATGGTATCCCAATTGGAAGCAGAAGAAAAGCTTTGGATGATGGAAACAGAAACCCAAAGAAGCAGCAAGCATCAAAATAAGATGGAAACACTCCAAAAATTTGCATTAAAATACCTTTCAAATCAAGAGCTGTCCTGCTGGCAAATCTGGAAACAGGTTGCAAGTGAATTAACCAGGTGTCTTCAGGGGAAGAGTTCCCAGTTATTACAAGGTGACTCTATTCAGGTTTCTGAAAATGAGAACAATATAATGAACCCTAAAGGAGATAGCTCTATTTATATTGAAAATCAAGAGTTTCCATTTTGGAGAACCCAGCATTCTTGCGGGAATACATATCTGAGTGAGTCACAGATTCAGAGTAGAGGTAAGCAAATTGATGTGAAAAATAACCTGCAAATACATGAAGACTTCATGAAGAAATCACCATTTCATGAGCATATTAAAACTGACACAGAACCAAAACCCTGCAAAGGTAATGAATATGGCAAAATCATTAGTGATGGCTCCAACCAGAAATTACCCTTAGGAGAGAAACCCCATCCATGTGGTGAGTGTGGAAGGGGCTTCAGTTATAGCCCAAGGCTTCCCCTTCATCCGAATGTTCACACAGGAGAAAAATGCTTCAGTCAAAGCTCACATCTGCGAACTCATCAGAGAATTCACCCAGGAGAGAAACTCAATAGATGTCATGAATCTGGTGATTGCTTCAATAAGAGCTCTTTTCATTCTTATCAATCTAATCATACAGGAGAGAAGTCTTATAGATGCGACAGTTGCGGCAAGGGATTCAGTAGCAGCACGGGTCTTATCATTCATTACAGAACTCATACTGGAGAGAAACCCTATAAATGCGAGGAATGTGGTAAATGCTTTAGTCAAAGTTCAAATTTTCAGTGCCATCAGAGAGTCCACACTGAAGAAAAACCATACAAATGCGAAGAGTGTGGTAAGGGCTTCGGTTGGAGTGTTAATCTCCGTGTTCACCAGAGGGTCCACAGGGGTGAGAAGCCCTATAAATGTGAGGAATGTGGTAAGGGCTTCACTCAGGCTGCACATTTTCACATCCATCAGAGAGTCCACACTGGAGAGAAACCCTACAAGTGTGATGTGTGTGGTAAGGGCTTCAGCCACAATTCACCATTAATATGCCATCGGAGAGTCCACACAGGAGAGAAGCCATACAAGTGTGAGGCGTGTGGGAAAGGCTTTACCCGTAATACAGATCTGCATATTCATTTCAGAGTTCACACGGGAGAGAAACCCTATAAATGTAAGGAGTGTGGTAAGGGCTTCAGTCAGGCTTCAAATCTTCAAGTCCATCAGAATGTCCACACTGGGGAGAAACGATTCAAGTGTGAAACGTGTGGGAAGGGCTTCAGTCAGTCCTCAAAGCTTCAAACCCATCAGCGAGTCCACACTGGAGAGAAACCATATAGATGTGATGTGTGTGGTAAGGACTTCAGTTATAGTTCAAATCTTAAACTACACCAAGTAATTCACACTGGAGAAAAACCATATAAATGTGAGGAATGTGGGAAGGGCTTCAGTTGGAGATCAAATCTTCATGCACATCAAAGAGTTCACTCAGGAGAAAAACCCTATAAATGTGAGCAGTGTGATAAGAGCTTCAGTCAGGCCATAGATTTTCGGGTACATCAGAGAGTCCATACTGGAGAGAAGCCATACAAATGTGGTGTCTGTGGTAAGGGCTTCAGTCAGTCCTCTGGTCTTCAATCCCATCAGAGAGTCCACACGGGGGAAAAGCCATACAAATGTGATGTGTGTGGAAAGGGCTTTAGATACAGTTCGCAGTTTATATACCATCAGAGAGGCCACACTGGAGAAAAACCTTACAAATGTGAAGAGTGTGGGAAAGGCTTTGGTAGGAGCTTGAATCTTCGCCATCATCAGAGGGTCCACACGGGAGAGAAACCCCATATATGTGAGGAGTGTGGTAAGGCCTTCagTCTCCCCTCAAATCTTCGAGTCCACCTGGGTGTTCACACCAGGGAAAAACTCTTTAAATGTGAAGAGTGTGGTAAAGGCTTCAGTCAGAGTGCACGTCTTGAAGCCCATCAGAGAGTCCACACTGGAGAAAAACCATACAAATGTGACATATGTGATAAGGACTTCCGTCACCGTTCACGTCTTACATATCATCAGAAAGTCCATACTGGTAAAAAGctta
MPSQNYDLPQKKQEKMTKFQEAVTFKDVAVVFSREELRLLDLTQRKLYRDVMVENFKNLVAVGHLPFQPDMVSQLEAEEKLWMMETETQRSSKHQNKMETLQKFALKYLSNQELSCWQIWKQVASELTRCLQGKSSQLLQGDSIQVSENENNIMNPKGDSSIYIENQEFPFWRTQHSCGNTYLSESQIQSRGKQIDVKNNLQIHEDFMKKSPFHEHIKTDTEPKPCKGNEYGKIISDGSNQKLPLGEKPHPCGECGRGFSYSPRLPLHPNVHTGEKCFSQSSHLRTHQRIHPGEKLNRCHESGDCFNKSSFHSYQSNHTGEKSYRCDSCGKGFSSSTGLIIHYRTHTGEKPYKCEECGKCFSQSSNFQCHQRVHTEEKPYKCEECGKGFGWSVNLRVHQRVHRGEKPYKCEECGKGFTQAAHFHIHQRVHTGEKPYKCDVCGKGFSHNSPLICHRRVHTGEKPYKCEACGKGFTRNTDLHIHFRVHTGEKPYKCKECGKGFSQASNLQVHQNVHTGEKRFKCETCGKGFSQSSKLQTHQRVHTGEKPYRCDVCGKDFSYSSNLKLHQVIHTGEKPYKCEECGKGFSWRSNLHAHQRVHSGEKPYKCEQCDKSFSQAIDFRVHQRVHTGEKPYKCGVCGKGFSQSSGLQSHQRVHTGEKPYKCDVCGKGFRYSSQFIYHQRGHTGEKPYKCEECGKGFGRSLNLRHHQRVHTGEKPHICEECGKAFSLPSNLRVHLGVHTREKLFKCEECGKGFSQSARLEAHQRVHTGEKPYKCDICDKDFRHRSRLTYHQKVHTGKKL
維真生物的(de)人(ren)源cDNA克隆中，雖然我們大約70%的克隆(long)與RefSeq或GeneBank所(suo)發表的基因列序(xu)完全一致,但也有些克隆含(han)有天然的單核(he)苷酸多(duo)態性而包含(han)一至多(duo)個SNPs, 因此與RefSeq或GeneBank所發表的列序會(hui)有些不(bu)同。我們強烈建議您在(zai)購買之前仔(zi)細(xi)將我們克隆的(de)序列與(yu)您所要求的(de)序列進行(xing)對比，以決定所購買的克隆(long)是否符(fu)合您的要(yao)求。如(ru)果我們預制的克隆(long)不符(fu)合您的要(yao)求，我們一般在1-2周內可以提供一個與您(nin)所要求的序(xu)列完(wan)全一致(zhi)的克(ke)隆(long)。我(wo)們(men)的每一個克(ke)隆(long)與RefSeq（2013）標準序列(lie)相比(bi)都含有一(yi)個完(wan)整(zheng)的ORF(Opening Reading Frame)。因為RefSeq數據庫會不斷更(geng)(geng)新，更(geng)(geng)新后的(de)標(biao)準序(xu)列將(jiang)來有(you)可能與我(wo)們現(xian)有(you)的(de)克隆序(xu)列產生(sheng)差(cha)異。我(wo)們所有(you)的(de)克隆產品都經(jing)過(guo)二代(dai)（NextGen）測(ce)序驗證，并(bing)且(qie)保證網站上克隆的序列與您收(shou)到的克隆序列完(wan)全一致。在未(wei)經維真生物書面(mian)給予授(shou)權前(qian)，人(ren)源ORF cDNA克隆不可轉售或(huo)贈予(yu)買方以外的第三方。同(tong)時, 在使用任何(he)維真(zhen)產品(pin)之前，買(mai)方(fang)(fang)有(you)責任(ren)(ren)確(que)認該基因的使用(yong)不(bu)會侵(qin)犯到第三(san)方(fang)(fang)的知識產(chan)權。此產(chan)品專門為(wei)基礎研究(jiu)所開(kai)發，不(bu)適(shi)用(yong)于任(ren)(ren)何治療(liao)或(huo)診斷應用(yong)。
400-077-2566
service@wzbio.cn
