產品名稱:人源cDNA克隆
產(chan)品規格:500μL甘油菌液(ye)
產(chan)品運輸條件:常(chang)溫(wen)
產品儲存條件和期限(xian):-80℃長期保存
產品特(te)點(dian):
? 人源基因C-端(duan)融合(he) Flag & 6×His 標簽;
? 含有(you)puromycin真核抗(kang)性基因,可篩選穩定轉染細胞株;
? 含有(you)卡那霉素原(yuan)核抗性基因,可篩選重組克隆(long),推(tui)薦(jian)使用濃度為30μg/ml。
產品使用:
將甘油菌(jun)在新鮮配制的(de)固體培養基(相應抗(kang)性)中(zhong)劃線,37℃培(pei)養箱培(pei)養過夜(ye),挑(tiao)取(qu)3個單克(ke)隆,分別(bie)接種(zhong)于相應抗性的液體培(pei)養基中,37℃搖床(chuang)培(pei)養過夜(ye),然(ran)后進(jin)行質粒的提取(qu)和驗(yan)證。
溫馨提(ti)示:考慮到實驗的嚴謹性,建議您(nin)拿到產品的第一時間進(jin)行測序確認。
ATGACGACCGAATCTGGATCAGACTCGGAATCCAAGCCGGACCAGGAGGCCGAGCCCCAGGAGGCGGCGGGGGCGCAGGGGCGCGCGGGGGCGCCCGTGCCGGAGCCGCCCAAGGAGGAGCAGCAGCAGGCCCTGGAGCAGTTCGCCGCCGCTGCAGCGCACAGCACCCCGGTGCGGAGGGAGGTCACTGACAAGGAACAGGAGTTTGCTGCCAGGGCTGCAAAACAGCTCGAATATCAGCAATTAGAAGACGATAAACTTTCTCAGAAATCATCTAGCAGTAAACTCTCTCGGTCTCCATTAAAGATTGTCAAAAAGCCTAAAAGCATGCAGTGCAAAGTGATACTTCTCGATGGATCAGAATATACCTGTGATGTAGAGAAACGCTCCAGAGGACAAGTGCTGTTTGATAAAGTGTGTGAACACTTGAACTTGCTAGAGAAAGACTACTTTGGGCTTACGTATCGAGATGCTGAAAACCAGAAGAATTGGTTGGACCCTGCTAAGGAAATAAAAAAACAGGTTCGAAGTGGTGCTTGGCACTTTTCATTTAATGTGAAATTTTATCCACCAGACCCTGCCCAACTATCTGAAGATATCACCAGGTACTACCTCTGCTTGCAGTTGCGAGATGACATCGTGTCCGGAAGGCTGCCCTGCTCCTTTGTTACCCTGGCCTTGCTGGGCTCCTACACTGTCCAGTCAGAGCTCGGAGACTATGACCCAGATGAATGTGGGAGCGATTACATTAGTGAGTTCCGCTTTGCACCAAACCACACTAAAGAACTGGAAGACAAAGTGATCGAGCTGCACAAGAGCCACAGAGGAATGACGCCAGCAGAAGCAGAGATGCATTTCTTGGAAAATGCCAAAAAATTATCAATGTATGGGGTAGATTTACATCATGCTAAGGACTCAGAAGGGGTAGAAATTATGTTAGGAGTTTGTGCAAGTGGTCTGTTGATATATCGCGACCGGCTGCGAATAAACAGATTTGCCTGGCCCAAGGTTCTAAAGATTTCATACAAACGGAACAACTTTTACATTAAGATCCGGCCGGGAGAGTTTGAACAATTTGAAAGCACCATTGGGTTTAAGCTGCCAAACCATCGAGCTGCCAAGCGTTTATGGAAAGTATGTGTTGAGCATCATACATTTTTCAGACTACTGTTACCAGAAGCACCTCCCAAGAAATTCCTAACCTTGGGTTCCAAGTTTCGTTATAGTGGCAGGACACAAGCGCAAACGAGAAGAGCCAGTGCGTTGATAGATCGCCCAGCACCTTACTTTGAACGCTCATCCAGCAAACGTTATACCATGTCTCGCAGCTTGGATGGAGAGGTTGGTACTGGCCAGTACGCCACAACAAAAGGCATCTCTCAGACCAACTTGATCACCACTGTGACTCCGGAGAAGAAGGCTGAGGAGGAGCGGGACGAGGAAGAGGACAAACGGAGGAAGGGGGAAGAAGTCACGCCCATCTCGGCCATCCGGCACGAGGGAAAGTCACCTGGGCTTGGCACTGACTCATGTCCCTTGTCACCCCCATCCACCCATTGTGCCCCCACATCTCCCACAGAGCTCCGTAGGAGGTGTAAGGAGAATGACTGCAAACTGCCAGGTTATGAGCCGTCCAGAGCTGAGCACCTGCCTGGAGAGCCCGCCTTGGACTCTGATGGCCCAGGGAGGCCTTACCTAGGGGATCAAGATGTGGCTTTTAGCTACAGACAGCAAACTGGCAAGGGGACCACCCTGTTCTCCTTCTCCTTGCAGCTCCCTGAGTCATTCCCCTCCCTCCTAGATGATGATGGATACCTCTCTTTCCCCAACCTTTCTGAAACCAACCTCCTGCCCCAGAGCTTGCAGCATTACCTCCCGATCCGCTCACCGTCCCTTGTGCCCTGTTTCCTCTTCATCTTTTTCTTTCTGCTGTCTGCCTCCTTCTCAGTGCCATACGCTCTCACTCTCTCCTTCCCTCTGGCTCTGTGCCTCTGCTACCTGGAGCCCAAGGCGGCCTCCTTGAGCGCCTCCCTAGACAATGACCCGAGTGACAGTTCAGAGGAAGAGACTGACAGTGAGCGCACGGACACCGCAGCCGACGGGGAGACCACCGCCACTGAGTCGGACCAGGAGGAAGATGCAGAGCTCAAGGCACAGGAGCTAGAAAAAACTCAAGATGACCTGATGAAACATCAAACCAACATTAGCGAGCTGAAAAGAACCTTCTTAGAAACCTCAACAGACACTGCCGTAACGAATGAATGGGAGAAGAGGCTTTCCACCTCCCCCGTGCGACTGGCCGCCAGGCAGGAGGATGCCCCCATGATCGAACCACTTGTCCCTGAAGAGACTAAGCAGTCTTCTGGGGAAAAGCTCATGGATGGCTCTGAAATCTTCAGTTTATTAGAGTCTGCGCGAAAACCAACAGAATTCATAGGAGGGGTTACTTCTACTTCTCAAAGCTGGGTTCAGAAAATGGAAACCAAGACGGAGTCCAGTGGAATAGAGACGGAACCCACCGTGCACCACCTGCCGCTTAGCACTGAGAAGGTGGTGCAGGAGACCGTGTTGGTGGAGGAGCGGCGTGTGGTGCACGCGAGTGGGGATGCTTCTTACTCGGCGGGAGACAGCGGGGATGCTGCAGCACAGCCCGCATTCACAGGCATTAAAGGGAAAGAGGGCTCTGCCTTGACGGAGGGGGCTAAAGAGGAAGGAGGGGAGGAGGTCGCTAAAGCTGTCCTGGAACAGGAAGAGACAGCCGCTGCTTCCCGTGAGCGACAAGAGGAGCAGAGTGCAGCCATCCACATTTCAGAAACTTTGGAACAAAAACCTCATTTTGAGTCCTCAACGGTGAAGACGGAAACCATCAGTTTTGGCAGTGTTTCACCGGGAGGAGTAAAGCTAGAAATTTCCACGAAGGAAGTGCCAGTAGTTCACACCGAAACCAAAACCATCACATATGAATCATCACAGGTCGATCCAGGCACAGATCTGGAGCCAGGCGTGCTGATGAGTGCACAGACGATCACATCTGAAACCACCAGTACCACCACCACTACGCACATCACCAAAACTGTGAAAGGGGGCATTTCAGAGACAAGAATTGAGAAGCGAATAGTCATCACGGGGGATGCAGACATTGACCATGACCAGGCGCTGGCTCAGGCAATTAAAGAGGCCAAAGAGCAGCACCCTGACATGTCAGTGACCAAAGTAGTGGTCCATAAAGAGACAGAGATCACACCAGAAGATGGAGAGGAT
MTTESGSDSESKPDQEAEPQEAAGAQGRAGAPVPEPPKEEQQQALEQFAAAAAHSTPVRREVTDKEQEFAARAAKQLEYQQLEDDKLSQKSSSSKLSRSPLKIVKKPKSMQCKVILLDGSEYTCDVEKRSRGQVLFDKVCEHLNLLEKDYFGLTYRDAENQKNWLDPAKEIKKQVRSGAWHFSFNVKFYPPDPAQLSEDITRYYLCLQLRDDIVSGRLPCSFVTLALLGSYTVQSELGDYDPDECGSDYISEFRFAPNHTKELEDKVIELHKSHRGMTPAEAEMHFLENAKKLSMYGVDLHHAKDSEGVEIMLGVCASGLLIYRDRLRINRFAWPKVLKISYKRNNFYIKIRPGEFEQFESTIGFKLPNHRAAKRLWKVCVEHHTFFRLLLPEAPPKKFLTLGSKFRYSGRTQAQTRRASALIDRPAPYFERSSSKRYTMSRSLDGEVGTGQYATTKGISQTNLITTVTPEKKAEEERDEEEDKRRKGEEVTPISAIRHEGKSPGLGTDSCPLSPPSTHCAPTSPTELRRRCKENDCKLPGYEPSRAEHLPGEPALDSDGPGRPYLGDQDVAFSYRQQTGKGTTLFSFSLQLPESFPSLLDDDGYLSFPNLSETNLLPQSLQHYLPIRSPSLVPCFLFIFFFLLSASFSVPYALTLSFPLALCLCYLEPKAASLSASLDNDPSDSSEEETDSERTDTAADGETTATESDQEEDAELKAQELEKTQDDLMKHQTNISELKRTFLETSTDTAVTNEWEKRLSTSPVRLAARQEDAPMIEPLVPEETKQSSGEKLMDGSEIFSLLESARKPTEFIGGVTSTSQSWVQKMETKTESSGIETEPTVHHLPLSTEKVVQETVLVEERRVVHASGDASYSAGDSGDAAAQPAFTGIKGKEGSALTEGAKEEGGEEVAKAVLEQEETAAASRERQEEQSAAIHISETLEQKPHFESSTVKTETISFGSVSPGGVKLEISTKEVPVVHTETKTITYESSQVDPGTDLEPGVLMSAQTITSETTSTTTTTHITKTVKGGISETRIEKRIVITGDADIDHDQALAQAIKEAKEQHPDMSVTKVVVHKETEITPEDGED
維真生物的人源(yuan)cDNA克(ke)隆(long)中,雖然我們(men)大(da)約70%的(de)克隆與RefSeq或GeneBank所發表的(de)基因列序完(wan)全一(yi)致,但也有些(xie)克隆含有天(tian)然的單(dan)核苷酸多(duo)(duo)態性而包(bao)含一至多(duo)(duo)個SNPs, 因此與RefSeq或GeneBank所發表的(de)列序會有些不同(tong)。我(wo)們強烈建議您在購買之前仔細將我(wo)們克(ke)隆(long)的(de)序(xu)列與您所要求(qiu)的(de)序(xu)列進行對比,以決(jue)定所購買的(de)克隆(long)是否符(fu)合(he)(he)您的(de)要求。如果我(wo)們預制(zhi)的(de)克隆(long)不符(fu)合(he)(he)您的(de)要求,我(wo)們一般在1-2周內可以提供一個與您(nin)所要求的(de)(de)序列(lie)完全一致的(de)(de)克(ke)隆。我們的(de)(de)每(mei)一個克(ke)隆與RefSeq(2013)標準序列相比(bi)都含有一(yi)個完整的ORF(Opening Reading Frame)。因為RefSeq數(shu)據庫會(hui)不斷更(geng)新(xin),更(geng)新(xin)后的標準序(xu)列(lie)將(jiang)來有(you)可(ke)能與我(wo)們(men)現有(you)的克隆(long)(long)序(xu)列(lie)產生差異。我(wo)們(men)所有(you)的克隆(long)(long)產品都經過二代(NextGen)測序(xu)驗(yan)證(zheng),并且保證(zheng)網站上克(ke)隆的序(xu)列與您收到的克(ke)隆序(xu)列完(wan)全(quan)一致。在(zai)未經維(wei)真(zhen)生物(wu)書面給予授權前,人源ORF cDNA克隆不可轉(zhuan)售或贈予買方以外的第三方。同時, 在使用(yong)任何維真(zhen)產品之前,買方有責任(ren)確(que)認該基(ji)因的(de)(de)使用(yong)不會侵犯到第三方的(de)(de)知識產權。此產品專門(men)為基(ji)礎研(yan)究所開發,不適用(yong)于任(ren)何治療(liao)或診(zhen)斷應(ying)用(yong)。