基因編號: NM_020717
產品編號: CH806090
基因名稱: SHROOM4; KIAA1202; SHAP
基因大小: 4482 bp
分子量: 164.34 kDa
酶切位點: SgfI_MluI
價格: 詢價
供貨時間: 電詢 400-077-2566
產品(pin)名稱：人源cDNA克隆
產品(pin)規格：500μL甘油菌(jun)液(ye)
產品運輸條件(jian)：常溫
產品儲存(cun)條件和期限：-80℃長期保存
產品(pin)特點：
? 人(ren)源基因C-端(duan)融(rong)合(he) Flag & 6×His 標簽；
? 含(han)有puromycin真核抗性(xing)基因，可篩選穩定(ding)轉(zhuan)染細胞株(zhu)；
? 含有卡(ka)那(nei)霉素原核(he)抗性基因，可篩選重組克隆，推薦(jian)使用濃(nong)度(du)為30μg/ml。
產(chan)品使(shi)用：
將甘(gan)油菌(jun)在新鮮配(pei)制(zhi)的固體(ti)培養基（相應抗(kang)性）中劃線，37℃培養箱培養過(guo)夜(ye)，挑取(qu)3個(ge)單克隆(long)，分(fen)別接(jie)種于相應抗(kang)性(xing)的液體培養基(ji)中，37℃搖床培養過(guo)夜(ye)，然后進行質(zhi)粒(li)的提(ti)取(qu)和驗證。
溫馨提示：考慮到(dao)實驗的嚴謹性，建(jian)議您拿(na)到(dao)產品的第(di)一時間進行測序確認。
ATGGAGAACCGGCCTGGGTCCTTCCAGTACGTCCCTGTGCAGCTGCAAGGGGGGGCACCCTGGGGCTTCACCCTTAAGGGGGGTCTGGAACACTGTGAGCCGCTCACAGTGTCTAAGATTGAAGATGGAGGCAAGGCAGCTTTGTCCCAGAAGATGAGGACTGGTGATGAGCTGGTGAATATCAATGGCACTCCATTATATGGCTCCCGCCAAGAGGCCCTCATTCTCATCAAAGGCTCCTTCCGGATTCTCAAGCTGATTGTCAGGAGGAGGAACGCCCCTGTCAGTAGGCCGCACTCATGGCATGTGGCCAAGCTGCTGGAGGGATGCCCTGAAGCAGCCACCACCATGCATTTCCCTTCTGAAGCCTTCAGCTTGTCCTGGCATTCTGGCTGCAACACAAGTGACGTGTGTGTGCAGTGGTGTCCACTCTCCCGGCATTGCAGCACCGAGAAAAGCAGCTCCATTGGCAGCATGGAGAGCCTGGAGCAACCAGGCCAAGCCACCTATGAGAGCCATCTGTTGCCTATTGACCAGAACATGTACCCTAACCAGCGTGACTCAGCCTACAGCTCCTTCTCGGCCAGCTCAAATGCTTCTGACTGTGCCCTTTCCCTCAGGCCAGAGGAGCCAGCCTCTACAGACTGCATCATGCAAGGCCCAGGGCCAACTAAGGCCCCCAGTGGCCGGCCTAATGTGGCTGAGACCTCAGGAGGTAGTCGGCGCACCAATGGGGGCCACCTGACCCCCAGCTCTCAGATGTCATCCCGTCCACAGGAGGGATACCAGTCAGGGCCCGCCAAAGCAGTCAGGGGCCCACCACAACCTCCAGTGAGGCGGGACAGCCTTCAGGCCTCCAGAGCCCAACTCCTCAATGGAGAGCAGCGCAGGGCATCTGAGCCTGTGGTCCCCTTGCCACAGAAGGAGAAACTGAGCTTAGAGCCTGTGCTACCCGCAAGGAACCCTAATAGGTTCTGTTGCCTCAGTGGGCATGACCAAGTGACAAGTGAGGGCCATCAGAACTGTGAGTTCAGTCAGCCTCCTGAATCCAGCCAACAGGGCTCTGAGCATCTACTGATGCAGGCCTCAACCAAAGCTGTTGGATCCCCAAAAGCCTGTGACAGAGCTTCCAGCGTGGATTCCAACCCACTCAATGAGGCTTCTGCAGAGCTAGCTAAGGCTTCTTTTGGCAGACCTCCACATCTCATAGGACCCACAGGGCATCGCCATAGTGCCCCTGAACAGCTGCTGGCATCCCACCTGCAGCATGTGCACCTTGATACCAGGGGCAGCAAAGGGATGGAGCTCCCACCCGTACAGGATGGGCACCAGTGGACTCTGTCCCCTTTGCACAGCAGCCACAAAGGGAAGAAAAGTCCATGCCCCCCTACAGGAGGAACCCATGACCAGTCCAGCAAAGAAAGAAAGACCAGACAAGTGGATGACAGGTCTTTAGTTTTGGGACACCAGAGCCAAAGCAGTCCCCCACATGGAGAGGCTGATGGACACCCCTCAGAAAAAGGTTTCCTGGACCCAAACAGAACAAGCAGAGCAGCCAGTGAATTGGCCAACCAGCAACCCTCTGCCTCTGGCTCCCTTGTTCAACAAGCCACGGACTGTTCTTCAACCACTAAAGCAGCTAGTGGCACAGAGGCAGGTGAAGAAGGGGACAGCGAGCCCAAGGAGTGCAGCCGGATGGGTGGTAGGCGAAGTGGAGGGACCCGGGGCCGCTCGATCCAAAACCGGCGGAAGAGTGAGCGTTTTGCTACCAATCTGCGTAATGAAATTCAGAGGAGGAAGGCCCAGCTCCAGAAAAGCAAGGGTCCCTTGTCACAGCTGTGTGACACTAAGGAGCCAGTGGAAGAGACCCAGGAGCCCCCAGAAAGTCCTCCACTCACTGCCTCTAACACATCTCTTCTATCTTCATGTAAAAAACCTCCCAGCCCCAGAGACAAGCTCTTCAACAAAAGCATGATGCTCAGAGCTAGGTCTTCCGAGTGCCTCAGCCAAGCCCCTGAGAGCCATGAATCTAGGACAGGCTTAGAGGGACGAATAAGCCCTGGCCAGAGGCCTGGCCAGTCCTCTTTGGGCCTGAACACCTGGTGGAAAGCACCTGACCCATCCTCCTCAGACCCTGAGAAAGCACATGCTCACTGTGGAGTCCGTGGAGGTCATTGGAGATGGTCTCCAGAGCATAATTCACAGCCACTTGTGGCAGCAGCCATGGAAGGCCCTTCCAACCCAGGTGACAACAAGGAATTGAAGGCTTCTACTGCTCAAGCTGGGGAGGATGCCATCCTCTTGCCTTTTGCAGACAGAAGAAAGTTCTTTGAAGAGAGTAGCAAATCCTTATCTACATCTCATTTGCCAGGTTTAACCACTCATAGCAACAAGACTTTTACCCAGAGACCAAAACCTATAGACCAAAACTTCCAGCCAATGAGCTCCAGCTGTAGGGAATTGAGGCGCCATCCCATGGACCAATCATATCATTCCGCAGACCAACCATATCATGCCACAGACCAATCATATCATTCCATGTCACCCCTTCAGTCAGAAACTCCCACTTACTCAGAATGTTTTGCAAGCAAAGGTCTAGAAAATTCCATGTGTTGTAAGCCACTACACTGTGGTGATTTTGATTACCACAGGACCTGCTCTTACTCCTGCAGTGTTCAAGGAGCTCTAGTCCATGATCCTTGCATTTATTGTTCTGGGGAAATCTGCCCTGCCTTGCTAAAGAGAAATATGATGCCAAATTGCTACAACTGCCGGTGCCACCACCACCAATGCATTCGGTGTTCAGTTTGCTATCATAATCCTCAGCACAGTGCCCTCGAGGACAGCAGCTTGGCACCTGGCAACACTTGGAAACCCAGGAAGCTGACAGTGCAGGAATTTCCTGGGGACAAATGGAATCCAATAACAGGAAACAGGAAGACCAGCCAGTCAGGGAGGGAAATGGCTCATTCCAAGACTAGCTTTTCATGGGCAACCCCTTTCCATCCTTGCCTTGAGAACCCAGCACTGGACTTGTCAAGCTACCGAGCAATTTCTTCTCTTGACCTCCTTGGAGACTTCAAACATGCTTTGAAAAAATCAGAGGAAACTTCAGTTTATGAGGAGGGGAGCTCCCTTGCCTCCATGCCCCACCCACTGCGCAGCCGTGCCTTCTCAGAGAGTCACATCAGCTTGGCGCCCCAAAGCACCCGGGCCTGGGGGCAGCATAGGAGGGAGCTCTTTAGCAAAGGTGATGAGACCCAGTCGGATCTTCTCGGAGCCAGGAAGAAGGCCTTTCCTCCTCCTCGCCCTCCTCCTCCCAACTGGGAGAAGTACAGGCTCTTTCGTGCAGCCCAGCAGCAGAAGCAGCAACAGCAGCAGCAGAAGCAACAGGAGGAGGAGGAGGAGGAGGAAGAAGAAGAAGAAGAGGAAGAGGAAGAGGAGGAGGAGGAGGCAGAGGAGGAGGAAGAGGAGCTGCCACCCCAGTATTTCAGTTCAGAAACCTCTGGTTCCTGTGCTCTCAATCCTGAGGAGGTCCTAGAGCAGCCACAACCCCTCAGCTTTGGCCACCTGGAGGGCTCGAGACAGGGTTCACAAAGTGTCCCAGCAGAGCAAGAATCCTTTGCACTCCATTCCAGTGATTTCTTGCCTCCAATAAGGGGTCACTTGGGATCTCAACCTGAGCAGGCTCAGCCCCCTTGCTACTATGGCATTGGTGGGCTTTGGAGGACATCGGGACAGGAAGCCACTGAATCCGCCAAACAAGAGTTTCAGCACTTTTCGCCTCCTTCAGGGGCCCCAGGAATCCCTACCTCTTACTCAGCTTATTACAATATTTCTGTGGCCAAGGCAGAGCTGCTGAACAAACTGAAAGACCAACCTGAGATGGCAGAGATTGGCCTAGGAGAGGAGGAAGTTGACCATGAACTGGCTCAAAAAAAGATACAGCTTATCGAAAGCATCAGCAGAAAACTTTCTGTCTTGCGGGAGGCCCAGCGAGGGCTGCTAGAGGACATCAATGCCAATTCTGCCCTTGGGGAGGAGGTGGAGGCCAACTTAAAAGCCGTCTGCAAATCCAATGAATTTGAAAAGTACCACTTGTTTGTTGGGGACCTGGACAAAGTGGTCAACCTGTTGCTGTCACTCTCTGGACGACTGGCCCGGGTGGAGAATGCTCTGAACAGCATCGATTCAGAGGCCAACCAGGAGAAGTTGGTACTGATAGAGAAGAAGCAGCAGCTGACGGGGCAGTTGGCAGATGCCAAGGAGCTGAAGGAGCACGTGGACCGCCGGGAGAAGTTGGTGTTTGGCATGGTCTCCCGCTACCTGCCTCAGGACCAGCTCCAAGATTACCAGCACTTTGTCAAGATGAAATCTGCTCTCATCATTGAACAGCGAGAGCTGGAGGAGAAGATCAAGCTCGGGGAAGAGCAACTCAAATGTCTCAGGGAGAGTCTACTCCTGGGGCCCAGCAATTTC
MENRPGSFQYVPVQLQGGAPWGFTLKGGLEHCEPLTVSKIEDGGKAALSQKMRTGDELVNINGTPLYGSRQEALILIKGSFRILKLIVRRRNAPVSRPHSWHVAKLLEGCPEAATTMHFPSEAFSLSWHSGCNTSDVCVQWCPLSRHCSTEKSSSIGSMESLEQPGQATYESHLLPIDQNMYPNQRDSAYSSFSASSNASDCALSLRPEEPASTDCIMQGPGPTKAPSGRPNVAETSGGSRRTNGGHLTPSSQMSSRPQEGYQSGPAKAVRGPPQPPVRRDSLQASRAQLLNGEQRRASEPVVPLPQKEKLSLEPVLPARNPNRFCCLSGHDQVTSEGHQNCEFSQPPESSQQGSEHLLMQASTKAVGSPKACDRASSVDSNPLNEASAELAKASFGRPPHLIGPTGHRHSAPEQLLASHLQHVHLDTRGSKGMELPPVQDGHQWTLSPLHSSHKGKKSPCPPTGGTHDQSSKERKTRQVDDRSLVLGHQSQSSPPHGEADGHPSEKGFLDPNRTSRAASELANQQPSASGSLVQQATDCSSTTKAASGTEAGEEGDSEPKECSRMGGRRSGGTRGRSIQNRRKSERFATNLRNEIQRRKAQLQKSKGPLSQLCDTKEPVEETQEPPESPPLTASNTSLLSSCKKPPSPRDKLFNKSMMLRARSSECLSQAPESHESRTGLEGRISPGQRPGQSSLGLNTWWKAPDPSSSDPEKAHAHCGVRGGHWRWSPEHNSQPLVAAAMEGPSNPGDNKELKASTAQAGEDAILLPFADRRKFFEESSKSLSTSHLPGLTTHSNKTFTQRPKPIDQNFQPMSSSCRELRRHPMDQSYHSADQPYHATDQSYHSMSPLQSETPTYSECFASKGLENSMCCKPLHCGDFDYHRTCSYSCSVQGALVHDPCIYCSGEICPALLKRNMMPNCYNCRCHHHQCIRCSVCYHNPQHSALEDSSLAPGNTWKPRKLTVQEFPGDKWNPITGNRKTSQSGREMAHSKTSFSWATPFHPCLENPALDLSSYRAISSLDLLGDFKHALKKSEETSVYEEGSSLASMPHPLRSRAFSESHISLAPQSTRAWGQHRRELFSKGDETQSDLLGARKKAFPPPRPPPPNWEKYRLFRAAQQQKQQQQQQKQQEEEEEEEEEEEEEEEEEEEEAEEEEEELPPQYFSSETSGSCALNPEEVLEQPQPLSFGHLEGSRQGSQSVPAEQESFALHSSDFLPPIRGHLGSQPEQAQPPCYYGIGGLWRTSGQEATESAKQEFQHFSPPSGAPGIPTSYSAYYNISVAKAELLNKLKDQPEMAEIGLGEEEVDHELAQKKIQLIESISRKLSVLREAQRGLLEDINANSALGEEVEANLKAVCKSNEFEKYHLFVGDLDKVVNLLLSLSGRLARVENALNSIDSEANQEKLVLIEKKQQLTGQLADAKELKEHVDRREKLVFGMVSRYLPQDQLQDYQHFVKMKSALIIEQRELEEKIKLGEEQLKCLRESLLLGPSNF
維真生(sheng)物(wu)的人源cDNA克隆中，雖然我們大(da)約70%的(de)克隆與RefSeq或GeneBank所發(fa)表的(de)基因列序完(wan)全(quan)一(yi)致,但也有些克隆含有天然(ran)的單核苷酸多態性而包含一至多個SNPs, 因此與RefSeq或GeneBank所發表的列序會有些不同。我們強烈建議您(nin)在購買之(zhi)前仔細將我們克隆的序列與(yu)您所要求(qiu)的序列進(jin)行對比，以(yi)決定所購買的克隆是(shi)否(fou)符(fu)(fu)合(he)您的要求。如果我(wo)們(men)預制的克隆不符(fu)(fu)合(he)您的要求，我(wo)們(men)一般在1-2周內可以提供一(yi)個與(yu)您所要求的(de)(de)序列完全一(yi)致的(de)(de)克(ke)隆(long)。我們的(de)(de)每一(yi)個克(ke)隆(long)與(yu)RefSeq（2013）標(biao)準序(xu)列相比都含有一個完整的ORF(Opening Reading Frame)。因為RefSeq數據庫會不斷更新，更新后的(de)標(biao)準序(xu)列(lie)將來有(you)可能與我們現有(you)的(de)克隆序(xu)列(lie)產生差(cha)異(yi)。我們所有(you)的(de)克隆產品都經過二代（NextGen）測序驗證，并(bing)且保(bao)證網站上克隆的序列(lie)與您收(shou)到的克隆序列(lie)完(wan)全(quan)一致。在未(wei)經維真生物書(shu)面給(gei)予授權前，人源(yuan)ORF cDNA克隆不可(ke)轉售或(huo)贈予買方以(yi)外的第三方。同時, 在使(shi)用(yong)任何維真產品之前，買方(fang)有(you)責任(ren)確(que)認(ren)該基因的使(shi)用(yong)(yong)不(bu)會(hui)侵犯到第三方(fang)的知識產權(quan)。此產品(pin)專門為基礎(chu)研究所開(kai)發(fa)，不(bu)適(shi)用(yong)(yong)于任(ren)何治療(liao)或診(zhen)斷應用(yong)(yong)。
400-077-2566
service@wzbio.cn
