產品名稱:人源cDNA克隆
產品規格:500μL甘油菌液
產品(pin)運(yun)輸條件(jian):常(chang)溫
產品儲存條件和(he)期限:-80℃長(chang)期保存
產品特點:
? 人源基因C-端融(rong)合 Flag & 6×His 標簽;
? 含有puromycin真核抗性(xing)基因,可篩選(xuan)穩定轉染細(xi)胞株;
? 含(han)有卡(ka)那霉素原核(he)抗性基因(yin),可篩選(xuan)重組克隆(long),推薦使用濃度為30μg/ml。
產品使(shi)用:
將甘油菌在(zai)新(xin)鮮配制的固體(ti)培養基(相應抗性)中劃(hua)線,37℃培養(yang)(yang)箱培養(yang)(yang)過夜,挑取(qu)3個單克隆,分別接(jie)種于(yu)相應(ying)抗性的(de)液體培養(yang)(yang)基(ji)中(zhong),37℃搖床(chuang)培養(yang)(yang)過夜,然后進行質粒的(de)提取(qu)和驗證。
溫馨提示:考慮(lv)到(dao)實驗的嚴謹性,建(jian)議您拿(na)到產品的第(di)一(yi)時間進行測序(xu)確(que)認。
CGCCATGTCCAAGAAAGGGCGAAATAAGGGCGAGAAGCCCGAGGCACTCATTGTTGCCCTTCAAGCTGCCAATGAAGACCTCAGGACCAAGCTCACAGACATTCAGATAGAGCTGCATCAAGAGAAGTCCAAGGTATCAAAGCTTGAAAGAGAGAAGACTCAAGAAGCGAAGAGGATTCGTGAGCTGGAGCAGCGCAAGCACACGGTGCTGGTGACAGAACTCAAAGCCAAGCTCCATGAGGAGAAGATGAAGGAGCTGCGGGCTGTGAGGGAGAACCTTATCAAGCAGCACGAGCAGGAAATGTCAAGGACGGTGAAGGTACGTGATGGAGAGATCCAGAGGCTCAAGTCTGCTCTCTGTGCTCTCCGCGACGGCAGCAGTGACAAAGTAAGGACAGCGCTCACCATTGAGGCCCGGGAGGAGGCCCGGAAACTGTTTGACACAGAGCGCCTTAAGCTCTTACAGGAAATTGCGGACCTGAAAACGGCCAAGAAGCAGGTGGACGAGGCTCTGAGCAATATGATCCAAGCAGATAAAATCAAGGCTGGGGACCTTCGGAGTGAGCATCAGTCCCACCAAGAAGCCATCTCGAAGATCAAGTGGGAGTCGGAGCGGGATATTCGGAGGCTGATGGATGAAATCAAAGCCAAGGACAGGATCATCTTTTCCCTGGAAAAGGAACTGGAGACCCAGACAGGCTATGTACAGAAACTCCAACTTCAGAAGGAGGCTTTGGACGAACAACTCTTTCTGGTCAAGGAGGCTGAGTGCAACATGAGCAGCCCAAAACGAGAAATTCCAGGAAGGGCAGGTGATGGTTCCGAACACTGCAGCAGTCCTGATTTGCGAAGAAATCAAAAGAGAATAGCTGAATTGAATGCCACTATAAGAAAATTAGAAGACAGGAATACCTTGCTTGGAGATGAACGAAATGAACTGTTAAAACGTGTGCGGGAAACCGAAAAGCAATGTAAACCTCTCCTGGAAAGGAACAAGTGCCTCGCCAAGAGAAACGATGAACTGATGGTGTCCTTGCAGCGCATGGAAGAAAAACTAAAAGCCGTTACCAAGGAAAATTCAGAAATGAGAGAAAAAATAACATCCCATCCACCCCTGAAGAAATTAAAATCTCTGAATGACCTCGACCAAGCTAATGAAGAACAAGAAACAGAGTTTCTAAAACTTCAGGTCATTGAGCAACAGAACATTATTGATGAGCTCACAAGGGACCGAGAAAAGCTCATCCGTAGAAGAAAGCATAGAAGAGGTTCCAAGCCAATTAAGAGGCCTGTTTTGGACCCGTTTATTGGCTATGATGAGGACTCTATGGATTCAGAGACATCATCCATGGCCTCATTTAGAACAGACAGAACACCAGCTACTCCTGATGATGACTTGGATGAAAGTTTAGCAGCTGAAGAATCTGAACTAAGATTTCGACAATTAACAAAAGAATATCAGGCCCTCCAAAGAGCATATGCCCTCCTACAGGAGCAGACGGGAGGCATCATCGACGCTGAACGAGAAGCCAAGGCTCAAGAACAGCTCCAAGCAGAGGTGCTAAGGTATAAAGCCAAAATTGAAGACCTGGAAGCGACTCTGGCTCAGAAAGGGCAGGATTCACACTGGGTAGAAGATAAACAACTTTTCATTAAGAGAAACCAGGAGCTTTTAGAAAAGATAGAAAAACAGGAGGCAGAAAATCACCGGTTACAACAAGAACTACAGGACGCCAGAGACCAGAATGAGCTGCTGGAGTTTCGAAACCTAGAGCTAGAAGAGAGAGAGAGACGATCCCCTCCATTTAATCTCCAAATTCACCCATTCTCAGATGGTGTGAGTGCTCTACAGATCTACTGTATGAAAGAAGGTGTTAAGGATGTGAACATCCCTGATCTCATAAAGCAGCTTGATATCTTGGGTGATAATGGGAATTTAAGAAATGAAGAACAAGTGGCCATAATTCAGGCCAGCACTGTGCTGTCCCTGGCAGAGAAGTGGATCCAGCAGATTGAAGGAGCTGAGGCTGCCCTACACCAGAAAATGATGGAATTGGAAAGTGACATGGAACAGTTCTGCAAAATAAAAGGCTATCTGGAGGAAGAACTAGACTACAGAAAACAAGCTCTTGACCAAGCATATATGAGAATCCAGGAACTAGAAGCTACTTTGTACAATGCTCTACAGCAAGAAACTGTTATCAAGTTTGGTGAATTATTAAGTGAAAAACAGCAAGAGGAGCTGAGGACGGCAGTAGAAAAGTTACGGCGGCAAATGCTGAGGAAGAGCAGAGAGTATGACTGTCAGATTCTTCAGGAGAGAATGGAGCTCTTACAGCAAGCCCATCAGAGAATTCGTGACTTAGAAGATAAAACAGACATCCAGAAAAGACAAATAAAAGACTTAGAAGAAAAGAGTAACCGAAAACATGGaa
MSKKGRNKGEKPEALIVALQAANEDLRTKLTDIQIELHQEKSKVSKLEREKTQEAKRIRELEQRKHTVLVTELKAKLHEEKMKELRAVRENLIKQHEQEMSRTVKVRDGEIQRLKSALCALRDGSSDKVRTALTIEAREEARKLFDTERLKLLQEIADLKTAKKQVDEALSNMIQADKIKAGDLRSEHQSHQEAISKIKWESERDIRRLMDEIKAKDRIIFSLEKELETQTGYVQKLQLQKEALDEQLFLVKEAECNMSSPKREIPGRAGDGSEHCSSPDLRRNQKRIAELNATIRKLEDRNTLLGDERNELLKRVRETEKQCKPLLERNKCLAKRNDELMVSLQRMEEKLKAVTKENSEMREKITSHPPLKKLKSLNDLDQANEEQETEFLKLQVIEQQNIIDELTRDREKLIRRRKHRRGSKPIKRPVLDPFIGYDEDSMDSETSSMASFRTDRTPATPDDDLDESLAAEESELRFRQLTKEYQALQRAYALLQEQTGGIIDAEREAKAQEQLQAEVLRYKAKIEDLEATLAQKGQDSHWVEDKQLFIKRNQELLEKIEKQEAENHRLQQELQDARDQNELLEFRNLELEERERRSPPFNLQIHPFSDGVSALQIYCMKEGVKDVNIPDLIKQLDILGDNGNLRNEEQVAIIQASTVLSLAEKWIQQIEGAEAALHQKMMELESDMEQFCKIKGYLEEELDYRKQALDQAYMRIQELEATLYNALQQETVIKFGELLSEKQQEELRTAVEKLRRQMLRKSREYDCQILQERMELLQQAHQRIRDLEDKTDIQKRQIKDLEEKSNRKHG
維(wei)真生物的人(ren)源cDNA克隆中,雖然我們大(da)約(yue)70%的克隆與RefSeq或GeneBank所發(fa)表的基因(yin)列序完全一致,但也有些克隆含有天然的單核苷酸多態性(xing)而包含一至多個SNPs, 因此(ci)與RefSeq或GeneBank所發表的列序會有(you)些不(bu)同。我(wo)們強(qiang)烈建議您在購買之前仔細(xi)將我們克隆的(de)(de)序(xu)(xu)列(lie)與您所要求的(de)(de)序(xu)(xu)列(lie)進(jin)行對比,以決定所購買的(de)克隆(long)是否符(fu)合您的(de)要求(qiu)(qiu)。如果(guo)我們預制的(de)克隆(long)不符(fu)合您的(de)要求(qiu)(qiu),我們一般在1-2周內可以提供(gong)一(yi)個(ge)(ge)與您(nin)所(suo)要求的(de)(de)序列完全一(yi)致的(de)(de)克隆(long)。我們的(de)(de)每一(yi)個(ge)(ge)克隆(long)與RefSeq(2013)標準序列相(xiang)比(bi)都含有一個完整的ORF(Opening Reading Frame)。因為RefSeq數據庫會不(bu)斷更新,更新后(hou)的(de)(de)標準序列將來有可能與我(wo)們(men)現有的(de)(de)克隆序列產生差(cha)異。我(wo)們(men)所有的(de)(de)克隆產品都(dou)經過二代(NextGen)測(ce)序驗證(zheng),并且保證(zheng)網站上克隆的序列與(yu)您收到的克隆序列完全一致。在未(wei)經維真生物書(shu)面給予授權前(qian),人源(yuan)ORF cDNA克隆(long)不可轉(zhuan)售(shou)或贈(zeng)予買方以外的第三方。同時(shi), 在使(shi)用任何(he)維真(zhen)產品(pin)之前,買方有責任(ren)確認該基因的使用(yong)(yong)不會侵犯到第三方的知識產權。此產品專門(men)為基礎研究所開發,不適(shi)用(yong)(yong)于(yu)任(ren)何治(zhi)療或(huo)診斷應用(yong)(yong)。