產品(pin)名(ming)稱:人源cDNA克隆
產品(pin)規格:500μL甘(gan)油菌(jun)液
產品運輸(shu)條(tiao)件:常(chang)溫
產品儲存條件和期(qi)限:-80℃長期(qi)保存(cun)
產品特點:
? 人(ren)源基因(yin)C-端融合(he) Flag & 6×His 標簽;
? 含有puromycin真(zhen)核抗性基因(yin),可(ke)篩選(xuan)穩定轉染細胞株;
? 含有卡(ka)那霉素原核抗性基因,可篩選(xuan)重組(zu)克(ke)隆,推薦使用濃度為30μg/ml。
產品使用:
將甘油菌在新鮮配制的固(gu)體(ti)培養基(相應抗性)中劃線(xian),37℃培(pei)養(yang)箱培(pei)養(yang)過夜(ye),挑(tiao)取3個(ge)單克隆,分別接種(zhong)于相(xiang)應抗性的液體培(pei)養(yang)基(ji)中,37℃搖床(chuang)培(pei)養(yang)過夜(ye),然后進(jin)行(xing)質粒的提取和(he)驗(yan)證。
溫馨提(ti)示(shi):考慮到實驗的嚴謹性(xing),建議(yi)您拿到產品的第一(yi)時間進行測(ce)序(xu)確認。
ATGGCTCGTCATTTGCGCGAATACCAGGACCTCCTCAACGTCAAGATGGCTCTGGATATAGAAATCGCTGCGTACAGAAAACTCCTGGAGGGTGAAGAGACTAGATTTAGCACATTTGCAGGAAGCATCACTGGGCCACTGTATACACACCGACCCCCAATCACAATATCCAGTAAGATTCAGAAACCCAAGGTGGAAGCTCCCAAGCTTAAGGTCCAACACAAATTTGTCGAGGAGATCATAGAGGAAACCAAAGTGGAGGATGAGAAGTCAGAAATGGAAGAGGCCCTGACAGCCATTACAGAGGAATTGGCCGTTTCCATGAAGGAAGAGAAGAAAGAAGCAGCAGAAGAAAAGGAAGAGGAACCCGAAGCTGAAGAAGAAGAAGTAGCTGCCAAAAAGTCTCCAGTGAAAGCAACTGCACCTGAAGTTAAAGAAGAGGAAGGGGAAAAGGAGGAAGAAGAAGGCCAGGAAGAAGAGGAGGAAGAAGATGAGGGAGCTAAGTCAGACCAAGCCGAAGAGGGAGGATCCGAGAAGGAAGGCTCTAGTGAAAAAGAGGAAGGTGAGCAGGAAGAAGGAGAAACAGAAGCTGAAGCTGAAGGAGAGGAAGCCGAAGCTAAAGAGGAAAAGAAAGTGGAGGAAAAGAGTGAGGAAGTGGCTACCAAGGAGGAGCTGGTGGCAGATGCCAAGGTGGAAAAGCCAGAAAAAGCCAAGTCTCCTGTGCCAAAATCACCAGTGGAAGAGAAAGGCAAGTCTCCTGTGCCCAAGTCACCAGTGGAAGAGAAAGGCAAGTCTCCTGTGCCCAAGTCACCAGTGGAAGAGAAAGGCAAGTCTCCTGTGCCGAAATCACCAGTGGAAGAGAAAGGCAAGTCTCCTGTGTCAAAATCACCAGTGGAAGAGAAAGCCAAATCTCCTGTGCCAAAATCACCAGTGGAAGAGGCAAAGTCAAAAGCAGAAGTGGGGAAAGGTGAACAGAAAGAGGAAGAAGAAAAGGAAGTCAAGGAAGCTCCCAAGGAAGAGAAGGTAGAGAAAAAGGAAGAGAAACCAAAGGATGTGCCAGAGAAGAAGAAAGCTGAGTCCCCTGTAAAGGAGGAAGCTGTGGCAGAGGTGGTCACCATCACCAAATCGGTAAAGGTGCACTTGGAGAAAGAGACCAAAGAAGAGGGGAAGCCACTGCAGCAGGAGAAAGAGAAGGAGAAAGCGGGAGGAGAGGGAGGAAGTGAGGAGGAAGGGAGTGATAAAGGTGCCAAGGGATCCAGGAAGGAAGACATAGCTGTCAATGGGGAGGTAGAAGGAAAAGAGGAGGTAGAGCAGGAGACCAAGGAAAAAGGCAGTGGGAGGGAAGAGGAGAAAGGCGTTGTCACCAATGGCCTAGACTTGAGCCCAGCAGATGAAAAGAAGGGGGGTGATAAAAGTGAGGAGAAAGTGGTGGTGACCAAAACGGTAGAAAAAATCACCAGTGAGGGGGGAGATGGTGCTACCAAATACATCACTAAATCTGTAACCGTCACTCAAAAGGTTGAAGAGCATGAAGAGACCTTTGAGGAGAAACTAGTGTCTACTAAAAAGGTAGAAAAAGTCACTTCACACGCCATAGTAAAGGAAGTCACCCAGAGTGAC
MARHLREYQDLLNVKMALDIEIAAYRKLLEGEETRFSTFAGSITGPLYTHRPPITISSKIQKPKVEAPKLKVQHKFVEEIIEETKVEDEKSEMEEALTAITEELAVSMKEEKKEAAEEKEEEPEAEEEEVAAKKSPVKATAPEVKEEEGEKEEEEGQEEEEEEDEGAKSDQAEEGGSEKEGSSEKEEGEQEEGETEAEAEGEEAEAKEEKKVEEKSEEVATKEELVADAKVEKPEKAKSPVPKSPVEEKGKSPVPKSPVEEKGKSPVPKSPVEEKGKSPVPKSPVEEKGKSPVSKSPVEEKAKSPVPKSPVEEAKSKAEVGKGEQKEEEEKEVKEAPKEEKVEKKEEKPKDVPEKKKAESPVKEEAVAEVVTITKSVKVHLEKETKEEGKPLQQEKEKEKAGGEGGSEEEGSDKGAKGSRKEDIAVNGEVEGKEEVEQETKEKGSGREEEKGVVTNGLDLSPADEKKGGDKSEEKVVVTKTVEKITSEGGDGATKYITKSVTVTQKVEEHEETFEEKLVSTKKVEKVTSHAIVKEVTQSD
維(wei)真生(sheng)物的人源(yuan)cDNA克(ke)隆中,雖然我們大(da)約(yue)70%的克隆(long)與RefSeq或GeneBank所發表(biao)的基因列序完全一(yi)致,但也(ye)有(you)些克隆(long)含有(you)天然(ran)的單核(he)苷酸(suan)多態(tai)性(xing)而包含一至多個SNPs, 因此與RefSeq或(huo)GeneBank所發表的列序會有些不(bu)同(tong)。我們強(qiang)烈(lie)建議您在購買(mai)之前仔細將我們克隆的(de)序(xu)列與您所要(yao)求的(de)序(xu)列進行對比,以決定所購買的克(ke)隆是否(fou)符(fu)合您(nin)的要求(qiu)。如果我(wo)們(men)預制的克(ke)隆不(bu)符(fu)合您(nin)的要求(qiu),我(wo)們(men)一般在1-2周(zhou)內可以提供一個與您所(suo)要求的(de)序(xu)列完全一致的(de)克隆(long)。我們的(de)每一個克隆(long)與RefSeq(2013)標準序(xu)列相比都含有一(yi)個完整的ORF(Opening Reading Frame)。因(yin)為RefSeq數據庫(ku)會不斷更新,更新后(hou)的標準(zhun)序(xu)列將來有(you)可能與我們(men)現有(you)的克隆序(xu)列產生差異。我們(men)所有(you)的克隆產品都(dou)經過二代(dai)(NextGen)測序驗證,并且保證網站上克(ke)隆的(de)序列(lie)與您收到的(de)克(ke)隆序列(lie)完(wan)全一致。在未經維真生物書(shu)面(mian)給予授權前,人源(yuan)ORF cDNA克隆(long)不可轉售或贈(zeng)予買方(fang)以外(wai)的第三方(fang)。同(tong)時, 在使用任(ren)何維真產品之前,買(mai)方有責任(ren)確認該基(ji)因的使用(yong)不(bu)會侵犯到(dao)第三方的知識產權。此產品專(zhuan)門為基(ji)礎研究所開發,不(bu)適用(yong)于任(ren)何治療或診斷(duan)應用(yong)。