基因編號: NM_144973
產品編號: CH899925
基因名稱: DENND5B; DKFZp686P1174; FLJ41648; FLJ43333; MGC24039
基因大小: 3825 bp
分子量: 140.25 kDa
酶切位點: SgfI_MluI
價格: 詢(xun)價
供貨時間: 電詢 400-077-2566
產(chan)品名稱(cheng)：人(ren)源cDNA克隆(long)
產品規(gui)格(ge)：500μL甘油菌液
產品(pin)運輸條件：常(chang)溫
產品(pin)儲存條(tiao)件和期(qi)限：-80℃長期(qi)保存
產品特點：
? 人源基因(yin)C-端融(rong)合 Flag & 6×His 標簽；
? 含有(you)puromycin真(zhen)核抗性基因，可篩選穩定轉染(ran)細(xi)胞(bao)株；
? 含有卡(ka)那霉素原核抗(kang)性基因，可篩選重組克(ke)隆，推(tui)薦使用濃度(du)為(wei)30μg/ml。
產品使用：
將甘油(you)菌(jun)在新(xin)鮮(xian)配制的固體培養基(ji)（相(xiang)應抗(kang)性）中(zhong)劃線，37℃培(pei)(pei)養(yang)(yang)(yang)箱培(pei)(pei)養(yang)(yang)(yang)過夜，挑(tiao)取3個單克隆，分別接種于(yu)相應抗性的(de)液體培(pei)(pei)養(yang)(yang)(yang)基中(zhong)，37℃搖床培(pei)(pei)養(yang)(yang)(yang)過夜，然后進(jin)行質(zhi)粒(li)的(de)提取和驗證。
溫馨提示：考(kao)慮到實驗的嚴(yan)謹(jin)性，建(jian)議您拿到(dao)產品的第一時間進(jin)行測序確認。
ATGAGCGGGAGCTGCGCGGCGCCCGGCCCGGGCTCGGGCTCCTCCCCGGCCGCCTGCCGCTTCGCGCACTACTTCGTGCTGTGCGGGATCGACGCGGACAGCGGGCTGGAGCCTGACGAGCTGGCGGGCGAAAATTTTGACCAGAGTCCTTTGAGAAGAACATTCAAATCCAAAGTTCTCGCCCACTATCCTCAGAATATAGAATGGAACCCTTTTGATCAAGATGCGGTGAACATGTTGTGCATGCCTAAAGGGCTATCTTTCAGGACACAAACGGACAATAAAGACCCCCAGTTTCACTCATTTATAATTACCAGGGAAGATGGTTCTCGCACCTATGGTTTTGTTCTCACTTTTTATGAAGAAGTTACAAGTAAGCAAATCTGCACAGCAATGCAGACACTTTACCAGATGCACAACGCTGAGCATTACAGCAGTGTGTATGCTTCATCTTCCTGCAGTATGGACTCATTGGCAAGTAGTCTTGATGAAGGAGATACAACTTCCCTTTTGAAACTCCAGCGATACAACTCCTATGATATTAGCAGAGACACCCTGTATGTTTCAAAAAGTATATGCTTGATCACACCGTTACCATTCATGCAGGCCTGCAAGAAATTCCTTATCCAGCTTTACAAGGCTGTTACCTCACAGCAGCCACCACCCTTGCCACTTGAAAGCTATATCCACAATATTCTTTATGAAGTACCCCTTCCACCTCCAGGGAGGTCACTGAAATTTTATGGTGTTTATGAACCTGTCATCTGCCAGAGGCCTGGGCCCAGTGAACTCCCCCTCTCTGATTACCCCCTTCGGGAGGCATTTGAGCTCCTGGGATTAGAGAACCTGGTGCAGGTGTTTACCTGTGTTCTTTTAGAGATGCAAATCCTTCTCTACTCACAAGATTATCAACGCCTGATGACTGTGGCAGAAGGCATCACCACACTTTTGTTCCCATTTCAATGGCAACATGTTTATGTGCCCATTCTACCTGCTTCTCTGCTACATTTTCTTGATGCTCCTGTCCCTTATCTGATGGGCCTTCAGTCAAAAGAAGGAACTGACCGTTCTAAACTAGAACTTCCTCAAGAGGCTAATTTGTGTTTTGTGGACATTGACAACCATTTTATTGAGTTGCCTGAAGAATTTCCACAGTTCCCCAATAAAGTGGATTTTATCCAAGAACTCTCTGAGGTTCTTGTTCAATTTGGGATCCCTCCTGAGGGCAGCCTACATTGCAGTGAGAGTACCAGCAAACTGAAGAATATGGTTCTGAAAGACTTGGTCAATGACAAAAAGAACGGCAATGTCTGTACTAATAACATCAGCATGTATGAGTTACTGAAGGGCAATGAAACCATAGCCCGCTTGCAGGCTCTGGCCAAGCGTACTGGTGTGGCTGTGGAAAAAATGGACCTCTCTGCTTCTCTGGGTGAAAAAGACAAGGATTTAAAACTGCATTGTGAAGAGGCAGAACTAAGGGACTACCAGCTCAATGTACAGCTCCGAGAGGTCTTTGCTAACCGTTTTACACAGATGTTTGCAGATTACGAAGCATTTGTCATTCAGACTGCCCAGGACATGGAATCCTGGCTGACCAACCGGGAACAGATGCAGAACTTTGACAAAGCTTCCTTTCTGTCTGACCAGCCTGAGCCTTACCTGCCATTTCTTTCACGCTTCATTGAAACACAGATGTTTGCCACCTTTATTGATAATAAAATTATGTCTCAGTGGGAAGAGAAAGATCCTTTGCTTCGGGTCTTTGACACTCGGATTGATAAGATAAGGCTGTATAATGTAAGGGCACCCACCTTGCGGACATCTATATATCAGAAATGCAGCACTTTAAAAGAAGCAGCCCAATCAATTGAGCAGAGACTGATGAAAATGGATCACACTGCAATCCACCCACATCTACTTGATATGAAAATTGGTCAAGGCAAATATGAGCAGGGGTTCTTTCCAAAGTTACAGTCCGATGTCTTGGCAACAGGACCAACCAGTAACAATCGCTGGGTAAGTCGGAGTGCCACTGCACAGCGCAGGAAAGAACGCCTTCGCCAGCATTCTGAGCATGTTGGGCTGGACAACGACTTGAGGGAGAAATATATGCAAGAGGCACGAAGTTTAGGAAAAAACCTGAGGCAACCCAAACTGTCAGACCTCTCTCCTGCAGTTATTGCACAGACCAACTGTAAATTCGTAGAAGGCTTATTAAAAGAATGTAGAATGAAGACAAAGCGCATGTTGGTGGAGAAGATGGGACATGAAGCGGTGGAACTTGGCCATGGAGAAGCAAACATCACCGGCCTGGAGGAGAACACCTTGATCGCCAGCCTTTGTGACCTGCTGGAGAGGATATGGAGCCATGGCTTGCAGGTCAAGCAGGGGAAGTCGGCTTTGTGGTCACATTTAATTCAATTTCAGGACAGAGAAGAGAAACAAGAGCACCTTGCAGAATCACCAGTTGCCCTCGGACCAGAAAGAAGAAAATCTGACTCAGGAGTTATGTTGCCAACGCTCAGGGTCTCTCTTATTCAGGACATGAGGCATATTCAAAACATGAGTGAGATCAAGACTGATGTTGGACGAGCTCGGGCGTGGATAAGACTGTCTCTAGAAAAGAAGCTCTTGTCCCAGCATCTTAAGCAGTTGCTTTCTAACCAACCACTCACCAAGAAGCTTTATAAGCGATATGCTTTTCTACGTTGCGAAGAAGAAAGAGAGCAGTTTCTTTACCACCTTCTTTCTCTCAATGCTGTGGACTATTTCTGCTTCACCAGTGTGTTCACCACTATCATGATTCCGTATAGGTCAGTGATCATCCCAATCAAAAAGCTGAGCAATGCAATAATCACATCAAACCCTTGGATCTGTGTATCAGGAGAGCTGGGAGACACAGGAGTAATGCAGATTCCCAAAAACCTCCTCGAAATGACCTTTGAGTGCCAGAACTTGGGGAAGCTGACCACTGTTCAGATTGGTCACGATAACTCAGGACTGTTAGCCAAATGGCTAGTGGATTGTGTCATGGTCAGAAATGAAATCACAGGACATACATACAGATTCCCATGTGGGCGGTGGCTGGGGAAAGGCATTGATGATGGGAGCCTGGAGAGAATTCTTATTGGAGAGTTGATGACATCAGCATCAGATGAAGATCTAGTAAAGCAGTGTCGGACTCCACCCCAGCAGAAGTCACCCACCACGGCTAGGAGATTGAGCATCACTTCACTGACAGGAAAAAACAACAAACCCAATGCTGGGCAGATACAAGAAGGAATTGGAGAAGCTGTGAACAATATTGTGAAACATTTTCATAAACCTGAAAAAGAGAGAGGAAGCCTCACCGTGTTGCTGTGTGGAGAAAATGGCCTGGTTGCAGCCCTTGAGCAAGTTTTCCACCATGGGTTCAAATCTGCCCGCATCTTTCACAAGAATGTCTTCATCTGGGACTTCATAGAGAAAGTGGTTGCTTATTTTGAAACAACTGACCAGATTCTAGATAATGAAGATGATGTCCTTATTCAGAAATCATCCTGCAAAACCTTCTGCCACTACGTAAATGCTATTAATACTGCACCCAGGAACATTGGGAAGGATGGCAAATTCCAGATTTTAGTTTGCCTTGGAACAAGGGATCGCCTGCTCCCACAGTGGATTCCATTGTTAGCTGAGTGTCCTGCCATCACTCGAATGTATGAAGAGAGCGCTCTCCTGCGAGACCGCATGACTGTCAACTCCCTTATCCGAATTCTGCAGACCATTCAGGACTTCACCATAGTCCTAGAAGGATCACTCATCAAAGGAGTGGATGTG
MSGSCAAPGPGSGSSPAACRFAHYFVLCGIDADSGLEPDELAGENFDQSPLRRTFKSKVLAHYPQNIEWNPFDQDAVNMLCMPKGLSFRTQTDNKDPQFHSFIITREDGSRTYGFVLTFYEEVTSKQICTAMQTLYQMHNAEHYSSVYASSSCSMDSLASSLDEGDTTSLLKLQRYNSYDISRDTLYVSKSICLITPLPFMQACKKFLIQLYKAVTSQQPPPLPLESYIHNILYEVPLPPPGRSLKFYGVYEPVICQRPGPSELPLSDYPLREAFELLGLENLVQVFTCVLLEMQILLYSQDYQRLMTVAEGITTLLFPFQWQHVYVPILPASLLHFLDAPVPYLMGLQSKEGTDRSKLELPQEANLCFVDIDNHFIELPEEFPQFPNKVDFIQELSEVLVQFGIPPEGSLHCSESTSKLKNMVLKDLVNDKKNGNVCTNNISMYELLKGNETIARLQALAKRTGVAVEKMDLSASLGEKDKDLKLHCEEAELRDYQLNVQLREVFANRFTQMFADYEAFVIQTAQDMESWLTNREQMQNFDKASFLSDQPEPYLPFLSRFIETQMFATFIDNKIMSQWEEKDPLLRVFDTRIDKIRLYNVRAPTLRTSIYQKCSTLKEAAQSIEQRLMKMDHTAIHPHLLDMKIGQGKYEQGFFPKLQSDVLATGPTSNNRWVSRSATAQRRKERLRQHSEHVGLDNDLREKYMQEARSLGKNLRQPKLSDLSPAVIAQTNCKFVEGLLKECRMKTKRMLVEKMGHEAVELGHGEANITGLEENTLIASLCDLLERIWSHGLQVKQGKSALWSHLIQFQDREEKQEHLAESPVALGPERRKSDSGVMLPTLRVSLIQDMRHIQNMSEIKTDVGRARAWIRLSLEKKLLSQHLKQLLSNQPLTKKLYKRYAFLRCEEEREQFLYHLLSLNAVDYFCFTSVFTTIMIPYRSVIIPIKKLSNAIITSNPWICVSGELGDTGVMQIPKNLLEMTFECQNLGKLTTVQIGHDNSGLLAKWLVDCVMVRNEITGHTYRFPCGRWLGKGIDDGSLERILIGELMTSASDEDLVKQCRTPPQQKSPTTARRLSITSLTGKNNKPNAGQIQEGIGEAVNNIVKHFHKPEKERGSLTVLLCGENGLVAALEQVFHHGFKSARIFHKNVFIWDFIEKVVAYFETTDQILDNEDDVLIQKSSCKTFCHYVNAINTAPRNIGKDGKFQILVCLGTRDRLLPQWIPLLAECPAITRMYEESALLRDRMTVNSLIRILQTIQDFTIVLEGSLIKGVDV
維真(zhen)生(sheng)物的人源cDNA克隆(long)中，雖然(ran)我們大約70%的克隆(long)與RefSeq或GeneBank所(suo)發表的基(ji)因(yin)列序(xu)完全一致,但也有(you)些克(ke)隆含有(you)天然的(de)單(dan)核(he)苷酸多態(tai)性而包含一至多個SNPs, 因此與RefSeq或(huo)GeneBank所發表的列(lie)序會有(you)些不同。我們強烈建議(yi)您在購買之前仔細將我們克隆的序(xu)列與您所要求(qiu)的序(xu)列進行(xing)對(dui)比，以決定(ding)所(suo)購買的(de)克隆是否符合(he)您(nin)的(de)要(yao)求。如(ru)果我(wo)們預制的(de)克隆不符合(he)您(nin)的(de)要(yao)求，我(wo)們一般在1-2周內可以提供一(yi)個與(yu)您(nin)所要求的序列(lie)完全(quan)一(yi)致的克隆(long)。我們的每一(yi)個克隆(long)與(yu)RefSeq（2013）標準序列相比都含(han)有一個完整的ORF(Opening Reading Frame)。因(yin)為RefSeq數(shu)據庫會不斷更新，更新后(hou)的標準序(xu)列將來有可能與我們現有的克隆序(xu)列產生差異。我們所有的克隆產品都經(jing)過二代（NextGen）測序(xu)(xu)驗證，并且保證網站(zhan)上克隆(long)(long)的序(xu)(xu)列與(yu)您收到的克隆(long)(long)序(xu)(xu)列完全(quan)一致。在未(wei)經(jing)維(wei)真生物書面給(gei)予授權前，人源ORF cDNA克隆不可轉售或贈予(yu)買方以外的(de)第三方。同時, 在(zai)使用任何維真產品之前，買方(fang)有責任(ren)確認(ren)該基因的使(shi)用(yong)(yong)不(bu)會侵犯到第三方(fang)的知識產權(quan)。此產品專(zhuan)門為基礎研究所開發，不(bu)適用(yong)(yong)于任(ren)何治療或診(zhen)斷應用(yong)(yong)。
