基因編號: NM_000356
產品編號: CH899928
基因名稱: TCOF1
基因大小: 4236 bp
分子量: 155.32 kDa
酶切位點: SgfI_MluI
價格: 詢價
供貨時間: 電詢 400-077-2566
產(chan)品名稱：人源cDNA克隆(long)
產品規格：500μL甘油菌液
產品運(yun)輸條件：常(chang)溫
產(chan)品儲存條(tiao)件和期限：-80℃長期保存(cun)
產品特點(dian)：
? 人源基因C-端融合 Flag & 6×His 標(biao)簽；
? 含有puromycin真核抗性基(ji)因，可(ke)篩(shai)選(xuan)穩定轉染細胞株；
? 含有卡那霉(mei)素原(yuan)核抗性基因，可篩(shai)選重(zhong)組克隆，推薦(jian)使用濃度為30μg/ml。
產品(pin)使用：
將甘油(you)菌(jun)在新鮮配(pei)制的固體培(pei)養基（相應抗性(xing)）中劃線，37℃培養(yang)(yang)(yang)箱培養(yang)(yang)(yang)過夜，挑取(qu)3個單克(ke)隆，分別接種于相應抗性的(de)液體培養(yang)(yang)(yang)基中，37℃搖床培養(yang)(yang)(yang)過夜，然后(hou)進行質粒的(de)提取(qu)和驗證。
溫馨提(ti)示：考(kao)慮到實驗的嚴謹性，建議您拿(na)到產品的第(di)一(yi)時間進行測序確認。
ATGGCCGAGGCCAGGAAGCGGCGGGAGCTACTTCCCCTGATCTACCACCATCTGCTGCGGGCTGGCTATGTGCGTGCGGCGCGGGAAGTGAAGGAGCAGAGCGGCCAGAAGTGTTTCCTGGCTCAGCCCGTAACCCTTCTGGACATCTATACACACTGGCAACAAACCTCAGAGCTTGGTCGGAAGCGGAAGGCAGAGGAAGATGCGGCACTGCAAGCTAAGAAAACCCGTGTGTCAGACCCCATCAGCACCTCGGAGAGCTCGGAAGAGGAGGAAGAAGCAGAAGCCGAAACCGCCAAAGCCACCCCAAGACTAGCATCTACCAACTCCTCAGTCCTGGGGGCGGACTTGCCATCAAGCATGAAAGAAAAAGCCAAGGCAGAGACAGAGAAAGCTGGCAAGACTGGGAATTCCATGCCACACCCTGCCACTGGGAAGACGGTGGCCAACCTTCTTTCTGGGAAGTCTCCCAGGAAGTCAGCAGAGCCCTCAGCAAATACTACGTTGGTCTCAGAAACTGAGGAGGAGGGCAGCGTCCCGGCCTTTGGAGCTGCTGCCAAGCCTGGGATGGTGTCAGCGGGCCAGGCCGACAGCTCCAGCGAGGACACCTCCAGCTCCAGTGATGAGACAGACGTGGAGGTAAAGGCCTCTGAAAAAATTCTCCAGGTCAGAGCTGCCTCAGCCCCTGCCAAGGGGACCCCTGGGAAAGGGGCTACCCCAGCACCCCCTGGGAAGGCAGGGGCTGTAGCCTCCCAGACCAAGGCAGGGAAGCCAGAGGAGGACTCAGAGAGCAGCAGCGAGGAGTCATCTGACAGTGAGGAGGAGACGCCAGCTGCCAAGGCCCTGCTTCAGGCGAAGGCCTCAGGAAAAACCTCTCAGGTCGGAGCTGCCTCAGCCCCTGCCAAGGAGTCCCCCAGGAAAGGAGCTGCCCCAGCGCCCCCTGGGAAGACAGGGCCTGCAGTTGCCAAGGCCCAGGCGGGGAAGCGGGAGGAGGACTCGCAGAGCAGCAGCGAGGAATCGGACAGTGAGGAGGAGGCGCCTGCTCAGGCGAAGCCTTCAGGGAAGGCCCCCCAGGTCAGAGCCGCCTCGGCCCCTGCCAAGGAGTCCCCCAGGAAAGGGGCTGCCCCAGCACCTCCTAGGAAAACAGGGCCTGCAGCCGCCCAGGTCCAGGTGGGGAAGCAGGAGGAGGACTCAAGAAGCAGCAGCGAGGAGTCAGACAGTGACAGAGAGGCACTGGCAGCCATGAATGCAGCTCAGGTGAAGCCCTTGGGGAAAAGCCCCCAGGTGAAACCTGCCTCTACCATGGGCATGGGGCCCTTGGGGAAAGGCGCCGGCCCAGTGCCACCCGGGAAGGTGGGGCCTGCAACCCCCTCAGCCCAGGTGGGGAAGTGGGAGGAGGACTCAGAGAGCAGTAGTGAGGAGTCATCAGACAGCAGTGATGGAGAGGTGCCCACAGCTGTGGCCCCGGCTCAGGAAAAGTCCTTGGGGAACATCCTCCAGGCCAAACCCACCTCCAGTCCTGCCAAGGGGCCCCCTCAGAAGGCAGGGCCTGTAGCCGTCCAGGTCAAGGCTGAAAAGCCCATGGACAACTCGGAGAGCAGCGAGGAGTCATCGGACAGTGCGGACAGTGAGGAGGCACCAGCAGCCATGACTGCAGCTCAGGCAAAACCAGCTCTGAAAATTCCTCAGACCAAGGCCTGCCCAAAGAAAACCAATACCACTGCATCTGCCAAGGTCGCCCCTGTGCGAGTGGGCACCCAAGCCCCCCGGAAAGCAGGAACTGCGACTTCTCCAGCAGGCTCATCCCCAGCTGTGGCTGGGGGCACCCAGAGACCAGCAGAGGATTCTTCAAGCAGTGAGGAATCAGATAGTGAGGAAGAGAAGACAGGTCTTGCAGTAACCGTGGGACAGGCAAAGTCTGTGGGGAAAGGCCTCCAGGTGAAAGCAGCCTCAGTGCCTGTCAAGGGGTCCTTGGGGCAAGGGACTGCTCCAGTACTCCCTGGGAAGACGGGGCCTACAGTCACCCAGGTGAAAGCTGAAAAGCAGGAAGACTCTGAGAGCAGTGAGGAGGAATCAGACAGTGAGGAAGCAGCTGCATCTCCAGCACAGGTGAAAACCTCAGTAAAGAAAACCCAGGCCAAAGCCAACCCAGCTGCCGCCAGAGCACCTTCAGCAAAAGGGACAATTTCAGCCCCTGGAAAAGTTGTCACTGCAGCTGCTCAAGCCAAGCAGAGGTCTCCATCCAAGGTGAAGCCACCAGTGAGAAACCCCCAGAACAGTACCGTCTTGGCGAGGGGCCCAGCATCTGTGCCATCTGTGGGGAAGGCCGTGGCTACAGCAGCTCAGGCCCAGACAGGGCCAGAGGAGGACTCAGGGAGCAGTGAGGAGGAGTCAGACAGTGAGGAGGAGGCGGAGACGCTGGCTCAGGTGAAGCCTTCAGGGAAGACCCACCAGATCAGAGCTGCCTTGGCTCCTGCCAAGGAGTCCCCCAGGAAAGGGGCTGCCCCAACACCTCCTGGGAAGACAGGGCCTTCGGCTGCCCAGGCAGGGAAGCAGGATGACTCAGGGAGCAGCAGCGAGGAATCAGACAGTGATGGGGAGGCACCGGCAGCTGTGACCTCTGCCCAGGTGATTAAACCCCCTCTGATTTTTGTCGACCCTAATCGTAGTCCAGCTGGCCCAGCTGCTACACCCGCACAAGCCCAGGCTGCAAGCACCCCGAGGAAGGCCCGAGCCTCGGAGAGCACAGCCAGGAGCTCCTCCTCCGAGAGCGAGGATGAGGACGTGATCCCCGCTACACAGTGCTTGACTCCTGGCATCAGAACCAATGTGGTGACCATGCCCACTGCCCACCCAAGAATAGCCCCCAAAGCCAGCATGGCTGGGGCCAGCAGCAGCAAGGAGTCCAGTCGGATATCAGATGGCAAGAAACAGGAGGGACCAGCCACTCAGGTGTCAAAGAAGAACCCAGCTTCCCTCCCACTGACCCAGGCTGCCCTGAAGGTCCTCGCCCAGAAAGCCAGTGAGGCTCAGCCTCCTGTTGCCAGGACCCAGCCTTCAAGTGGGGTTGACAGTGCTGTGGGAACACTCCCTGCAACAAGTCCCCAGAGCACCTCCGTCCAGGCCAAAGGGACCAACAAGCTCAGAAAACCTAAGCTTCCTGAGGTCCAGCAGGCCACCAAAGCCCCTGAGAGCTCAGATGACAGTGAGGACAGCAGCGACAGTTCTTCAGGGAGTGAGGAAGATGGTGAAGGGCCCCAGGGGGCCAAGTCAGCCCACACGCTGGGTCCCACCCCCTCCAGGACAGAGACCCTGGTGGAGGAGACCGCAGCAGAGTCCAGCGAGGATGATGTGGTGGCGCCATCCCAGTCTCTCCTCTCAGGTTATATGACCCCTGGACTAACCCCAGCCAATTCCCAGGCCTCAAAAGCCACTCCCAAGCTAGACTCCAGCCCCTCAGTTTCCTCTACTCTGGCCGCCAAAGATGACCCAGATGGCAAGCAGGAGGCAAAGCCCCAACAGGCAGCAGGCATGTTGTCCCCTAAAACAGGTGGAAAAGAGGCTGCTTCAGGCACCACACCTCAGAAGTCCCGGAAGCCCAAGAAAGGGGCTGGGAACCCCCAAGCCTCAACCCTGGCGCTGCAAAGCAACATCACCCAGTGCCTCCTGGGCCAACCCTGGCCCCTGAATGAGGCCCAGGTGCAGGCCTCAGTGGTGAAGGTCCTGACTGAGCTGCTGGAACAGGAAAGAAAGAAGGTGGTGGACACCACCAAGGAGAGCAGCAGGAAGGGCTGGGAGAGCCGCAAGCGGAAGCTATCGGGAGACCAGCCAGCTGCCAGGACCCCCAGGAGCAAGAAGAAGAAGAAGCTGGGGGCCGGGGAAGGTGGGGAGGCCTCTGTTTCCCCAGAAAAGACCTCCACGACTTCCAAGGGGAAAGCAAAGAGAGACAAAGCAAGTGGTGATGTCAAGGAGAAGAAAGGGAAGGGGTCTCTTGGCTCCCAAGGGGCCAAGGACGAGCCAGAAGAGGAGCTTCAGAAGGGGATGGGGACGGTTGAAGGTGGAGATCAAAGCAACCCAAAGAGCAAGAAGGAGAAGAAGAAATCCGACAAGAGAAAAAAAGACAAAGAAAAAAAAGAAAAGAAGAAGAAAGCAAAAAAGGCCTCAACCAAAGATTCTGAGTCACCGTCCCAGAAGAAAAAGAAGAAAAAGAAGAAGACAGCAGAGCAGACTGTA
MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVSKKNPASLPLTQAALKVLAQKASEAQPPVARTQPSSGVDSAVGTLPATSPQSTSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDGEGPQGAKSAHTLGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNITQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQPAARTPRSKKKKKLGAGEGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGTVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV
維真生物的人源cDNA克(ke)隆(long)中，雖然(ran)我們大約70%的克隆與RefSeq或(huo)GeneBank所發(fa)表的(de)基因列序完全一致,但(dan)也有些(xie)克(ke)隆含(han)(han)有天(tian)然的單核苷(gan)酸多態(tai)性而包含(han)(han)一至多個SNPs, 因此與RefSeq或GeneBank所發表的列序會有些(xie)不(bu)同。我們強烈建議您在購買之前仔(zi)細將我(wo)們克(ke)隆的序列(lie)與(yu)您所要(yao)求的序列(lie)進(jin)行(xing)對比，以決定所購買(mai)的克隆(long)(long)是否符(fu)合您(nin)的要求(qiu)。如果我們預制的克隆(long)(long)不符(fu)合您(nin)的要求(qiu)，我們一(yi)般在1-2周內可(ke)以提供(gong)一(yi)個(ge)與(yu)您(nin)所要求的序列完全(quan)一(yi)致的克隆(long)(long)。我們的每一(yi)個(ge)克隆(long)(long)與(yu)RefSeq（2013）標準序列相比(bi)都含有一個完整(zheng)的(de)ORF(Opening Reading Frame)。因為RefSeq數(shu)據庫會不斷更新(xin)(xin)，更新(xin)(xin)后的(de)(de)標準序列(lie)將來有可能(neng)與我們現(xian)有的(de)(de)克隆序列(lie)產生差異。我們所有的(de)(de)克隆產品都經過二代（NextGen）測序驗證(zheng)，并且保證(zheng)網(wang)站上克(ke)隆的序列與您收(shou)到的克(ke)隆序列完全一致。在未(wei)經維真生物書面(mian)給予授權(quan)前，人源ORF cDNA克(ke)隆不可轉售或(huo)贈予買方(fang)以外(wai)的第三方(fang)。同時, 在使用任何維真產品之前，買方有責任(ren)確認該基因的使用(yong)不(bu)(bu)會侵犯(fan)到(dao)第三方的知識(shi)產(chan)權。此(ci)產(chan)品(pin)專(zhuan)門為(wei)基礎研究所開發，不(bu)(bu)適用(yong)于任(ren)何治療或診(zhen)斷應用(yong)。
