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Ready-to-package AAV shRNA clones，4 predesigned shRNA in pAV-U6-GFP vector + 1 negative control with scrambled sequences

Ready-to-package AAV shRNA clones，4 predesigned shRNA in pAV-U6-GFP vector + 1 negative control with scrambled sequences

基因編號: NM_001042784
產品編號: SH825094
基因名稱: CCDC158; FLJ25770; MGC35086
基因大小: 3342 bp
價格: 3500
促銷價: 3000
供貨時間: 1-2 weeks

  • 產品信息
  • DNA序列
  • siRNAs
  • 免責聲明
產品名稱： 人源shRNA克隆
產品規格： 各500μl甘油菌液+5ug質粒
產品運輸條件：常溫

產品儲存條件和期限：

菌液：-80℃，長(chang)期；

質粒：-20℃，長期。

產品特點：

? 帶有GFP，可直觀轉染效率；
? 含有(you)氨芐青霉素（Ampicillin ）原(yuan)核抗性基因，可篩選重組克隆(long)，推薦(jian)使用濃度為(wei)50μg/ml。

產品使用：

將甘油菌在新鮮配制的固體培養基（相應抗性）中劃線，37℃培養箱培養過夜，挑取3個單克隆，分別接種于相應抗性的液體培養基中，37℃搖床培養過夜，然后進行質粒的提取和驗證。或者(zhe)，直接用(yong)質粒(li)進行轉化。PS：產(chan)品(pin)質粒濃(nong)度(du)在100-200ng/ul之間，若(ruo)需要更精準(zhun)的(de)質粒濃度，請務必驗證(zheng)。

ATGGAATCAAAAGCTTGGGAATCAAATAATGAAGATCTTTTATCAAGTAGTGGTGTCACATCTAATGGAGGTTCTTCAAGTTCATTTTTTGTGTCATCTATTCGTGGTACAATAATTGAAAACACATCTTCAGCTGGGACTTTGACACAGGTTCCTTTTTTCCCTAAATATGAAGTGGAACTTGATTCTCCTAGAAAAATCATCCCATCTCCTGGAAAGGAACACTTTGAACGTGTTTTGGAAGAATATTCACATCAAGTCAAAGATTTACAGAGAAGACTAAATGAAAGCAATGAATTGCATGAGAAACAAAAGTTTTATTTGAGGCAGTCAGTCATTGATTTGCAAACAAAACTTCAGGAGATGCAAATGGAGAGAGATGCTATGGCTGACATCAGACGAAGGGAGAGTCAGTCCCAGGAGGATTTAAGAAATCAGCTTCAAAATACAGTTCATGAACTTGAAGCTGCCAAATGCCTTAAAGAGGACATGCTGAAAGACAGCAACACACAGATAGAGCAACTACGAAAAATGATGCTTAGTCATGAGGGAGTGCTTCAAGAAATCCGGTCAATCCTAGTTGACTTTGAAGAAGCCTCAGGCAAAAAAATATGTGAACATGACAGCATGTCTACTCTGCACTTCCGCAGCTTGGGCTCAGCTATTAGTAAAATACTAAGAGAATTAGACACAGAGATTTCTTATCTTAAAGGGAGGATATTTCCAGTAGAGGATCAACTTGAAGCACTGAAATCTGAATCACAGAACAAAATAGAACTACTTCTGCAACAACACCAAGATAGGATTGAGCAGTTAATAAGTGAACATGAAGTTGAAATAACAGGACTTACTGAGAAAGCTAGCAGTGCTCGAAGCCAAGCCAATAGTATCCAGAGTCAAATGGAAATCATTCAAGAGCAAGCAAGAAACCAAAACTCTATGTATATGCGTCAGCTCAGCGATCTGGAATCTACTGTTTCTCAGCTACGTTCTGAATTAAGGGAAGCCAAAAGGATGTATGAAGACAAGACAGAAGAGCTGGAAAAGCAGTTAGTCCTTGCCAACTCAGAGCTAACTGAAGCCCGGACAGAGCGTGATCAATTCAGTCAGGAATCTGGAAATTTAGATGATCAACTTCAAAAGCTGTTGGCTGATCTACACAAAAGGGAGAAGGAGCTGAGTCTGGAGAAGGAGCAGAATAAGCGTCTGTGGGACCGAGACACAGGCAACAGCATCACCATTGACCACCTGCGGCGGGAACTGGACAACCGGAACATGGAGGTGCAGCGCCTGGAAGCCCTGCTCAAGGCCTTGAAGAGCGAGTGTCAGGGCCAGATGGAGCGGCAGATGGCAGCAATTCAAGGAAAGAATGAAAGTCTAGAAAAAGTATCCTCCTTGACTGCTCAGCTTGAATCCACCAAAGAGATGCTGCGCAAAGTAGTAGAAGAGTTGACAGCCAAGAAAATGACTCTGGAGAGCTCAGAGAGGACAATATCGGACCTGACAACTTCTCTCCAGGAAAAAGAGAGAGCCATCGAGGCTACCAATGCAGAGATCACAAAGCTCCGCTCCCGGGTGGACTTGAAATTGCAGGAGCTGCAACACTTGAAAAATGAAGGGGATCATCTCAGAAATGTGCAGACAGAATGTGAGGCCCTCAAACTGCAGATGACAGAGAAGGACAAGGTGATCGAGATTCTGCGACAGCAGATTGAGAACATGACACAGCTGGTGGGCCAGCATGGACGAACTGCTGGAGCTATGCAAGTAGAAAAAGCTCAACTGGAGAAAGAAATTAATGATAGGCGGATGGAACTAAAGGAACTTAAGATATTAAAAGATAAAAAAGATGCAAAGATCCGGGAGCTTGAGGCCAGAGTGAGTGACTTGGAGCTGGAAAAGGTGAAGCTGGTGAATGCAGGCTCTGAGCGGCTCCGTGCAGTGAAGGACATCAAACAAGAGAGAGATCAATTATTAAATGAGGTGAAAACAAGTAGGAGTGAATTAAACAATCTTTCAGAGGAGTATGAAGTCTTAAAAAGGAATTTCCGAAACAAAAGTGAAGAAATGGAAATGACTACAAATAAGTTGAAAATGCAATTAAAATCTGCACAGTCTGAACTAGAACAGACAAGAAATACATTAAAGTCAATGGAAGGATCTGATGGTCATGCTATGAAAGTGGCAATGGGGATGCAAAAGCAAATCACAGCCAAAAGAGGTCAGATAGATGCCCTTCAGAGCAAGATACAGTTTTTGGAAGAGGCAATGACAAATGCAAATAAGGAGAAACATTTTCTGAAAGAAGAGAAAAGTAAACTCAGTCAGGAATTGAGTACTGTTGCCACAGAAAAAAACAAGATGGCTGGGGAGTTGGAAGTTCTGCGATCTCAGGAACGCCGTTTGAAAGAAAAGGTTACTAATATGGAAGTGGCTCTGGATAAGGCATCTTTGCAGTTTGCAGAATGTCAAGATATAATACAGCGTCAGGAGCAAGAATCAGTGCGCTTAAAACTTCAACACACTTTGGATATAAAAGAACTTCAGGGCCCTGGATACACCTCAAATTCTTCATTGAAACCACGCCTTCTCCAGCCAGCATCTGTTACTCGTTCTCATTCTAATGTACCATCTTCGCAGTCTACAGCCAGCTTCCTGTCTCATCACTCTACAAAAGCTAACACACTGAAGGAAGATCCAACAAGGGACCTGAAACAGCTTCTCCAAGAGTTGAGAAGCGTGATCAATGAGGAGCCAGCTGTGTCTCTGAGCAAGACAGAGGAAGATGGAAGAACATCTCTGGGAGCCTTAGAGGATAGGGTAAGAGATTGCATTACTGAATCCAGCCTGAGATCAGACATGTGCCATAGAAGCAACAACTCGTTGAGGGACTCCACTGAAGGAAGCAAATCAAGTGAAACTCTTAGTAGAGAGCCAGTCACATTACACGCAGGAGACAGGGAAGATCCCTCTGGTTGCTTCACATTCACATCTGCAGCAAGTCCATCTGTGAAAAACTCAGCTTCTCGTTCATTCAATTCTTCTCCTAAGAAGTCTCCAGTGCACTCACTCCTAACTAGTTCAGTTGAAGGTTCAATAGGTTCCACATCACAGTATAGATCTGCCAAACCTATTCATTCATCTGATTCTGTTAAAGATTCACAGTCTCCGCCAATAGAAACAACAGGAAAAACATGCAGGAAGCTTCAGAACAGACTAGAAAGCTTGCAAACTCTGGTAGAAGATTTACAGCTGAAGAACCAAGCAATGTCTTCAATGATCAGAAATCAAGAAAAGAGGATACAGAAAGTAAAAGACCAGGAAAAAATGTTACTAAAA

siRNA1： AATGGAGGTTCTTCAAGTTCATT
siRNA2： AAAGGGAGGATATTTCCAGTAGA
siRNA3： AATTCAAGGAAAGAATGAAAGTC
siRNA4： AAGGACATCAAACAAGAGAGAGA

維真生(sheng)物的(de)產品僅(jin)限用于(yu)研究，不可用于(yu)包括(kuo)人類體外診斷和治療在內的(de)其(qi)他用途。

維真生物的產品，不得修改和轉售給任何第三方，未經維真生物的書面批準，不得將產品用于向其他第三方提供服務或用于制造商品化產品。

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